分析与检测

基于多元变量统计分析法分析1-甲基环丙烯处理对蓝莓果实冷藏品质的影响

  • 张瑜瑜 ,
  • 洪永忠 ,
  • 用成健 ,
  • 陈泽斌 ,
  • 刘佳妮 ,
  • 苏源
展开
  • (昆明学院 农学与生命科学学院,云南 昆明,650214)
第一作者:硕士,副教授(陈泽斌副教授为通信作者,E-mail:445648238@qq.com)

收稿日期: 2021-10-26

  修回日期: 2021-12-09

  网络出版日期: 2023-02-15

基金资助

国家自然科学基金(42067009);云南省高校联合基金面上项目(2017FH001-040;2017FH001-025)

The effect of 1-methylcyclopropene treatment on refrigerated quality of blueberry fruit was analyzed based on multivariate statistical analysis

  • ZHANG Yuyu ,
  • HONG Yongzhong ,
  • YONG Chengjian ,
  • CHEN Zebin ,
  • LIU Jiani ,
  • SU Yuan
Expand
  • (College of Agronomy and Life Sciences, Kunming University, Kunming 650214, China)

Received date: 2021-10-26

  Revised date: 2021-12-09

  Online published: 2023-02-15

摘要

以“夏普蓝”蓝莓果实为实验材料,用添加量分别为0.5、1.0、1.5 μL/L的1-甲基环丙烯(1-methylcyclopropene,1-MCP)熏蒸处理,探讨冷藏期间蓝莓果实的品质变化,结合多元变量统计分析法进行综合分析,研究1-MCP对蓝莓果实冷藏品质的影响。结果表明,与对照组相比,1-MCP处理能明显减缓蓝莓果实可滴定酸和维生素C含量的下降,延缓相对电导率和呼吸速率上升,推迟呼吸高峰和乙烯释放高峰出现,延缓多酚氧化酶(polyphenol oxidase,PPO)和过氧化物酶(peroxidase,POD)活性下降,抑制丙二醛(malondialdehyde,MDA)含量上升,能有效延缓蓝莓果实衰老。主成分分析提取出特征值大于1的2个因子,累积方差贡献率达到93.262%,其可以充分反映原始数据的重要信息。相关性分析可知,相对电导率与呼吸强度、乙烯释放量、PPO、POD和MDA含量两两之间呈显著甚至极显著的正相关。根据蓝莓果实冷藏的品质指标,将对照组和处理组进行聚类分析,可区分为3组。综上可知,1.0 μL/L 1-MCP熏蒸处理对蓝莓果实采后冷藏品质的效果最佳。

本文引用格式

张瑜瑜 , 洪永忠 , 用成健 , 陈泽斌 , 刘佳妮 , 苏源 . 基于多元变量统计分析法分析1-甲基环丙烯处理对蓝莓果实冷藏品质的影响[J]. 食品与发酵工业, 2023 , 49(2) : 257 -263 . DOI: 10.13995/j.cnki.11-1802/ts.029827

Abstract

To explore the quality changes of blueberry fruits during cold storage, “Sharp Blue” blueberry fruits were fumigated with 1-methylcyclopropene(1-MCP) at concentrations of 0.5, 1.0, and 1.5 μL/L, respectively. Combined with multivariate statistical analysis, the effect of 1-MCP on the quality of blueberry fruits during cold storage was studied. Results showed that compared with the control group (CK), 1-MCP treatment could significantly maintain the decrease of titratable acid and vitamin C contents, delay the increase of relative conductivity and respiration rate, delay the peak of respiration and ethylene release, and delay the decrease of polyphenol oxidase (PPO) and peroxidase (POD) activities. Inhibiting the increase of malondialdehyde (MDA) content can effectively delay the senescence of blueberry fruit. Principal component analysis extracted two factors with characteristic values greater than 1, and the cumulative variance contribution rate reached 93.262%, which could fully reflect the important information of the original data. Correlation analysis showed that the relative conductivity was significantly positively correlated with respiratory intensity, ethylene release, PPO, POD, and MDA. According to the quality indexes of frozen blueberry fruit, the control group and the treatment group were analyzed by cluster analysis, which could be divided into 3 groups. In conclusion, 1.0 μL/L 1-MCP fumigationhad the best effect on the postharvest refrigerated quality of blueberry fruits.

参考文献

[1] 姜爱丽. 蓝莓果实采后生理生化代谢及调控研究[D].沈阳:沈阳农业大学, 2011.
JIANG A L.Study on physio-biochemical metabolism and its regulation of postharvest blueberry fruits[D].Shenyang: Shenyang Agricultural University, 2011.
[2] MAHAJAN B V C, SINGH K, DHILLON W S.Effect of 1-methylcyclopropene(1-MCP) on storage life and quality of pear fruits[J].Journal of Food Science & Technology, 2010, 47(3):351-354.
[3] SISLER E C, SEREK M.Inhibitors of ethylene responses in plants at the receptor level:Recent developments[J].Physiologia Plantarum, 1997, 100(3):577-582.
[4] 陈莲, 王璐璐, 林河通, 等.1-MCP延缓采后台湾青枣果实衰老及其与能量代谢的关系[J].热带作物学报, 2017, 38(1):175-182.
CHEN L, WANG L L, LIN H T, et al.Delaying senescence of harvested Ziziphus mauritiana lamk fruit by postharvest 1-methylcyclopropene (1-MCP) treatment and its relation to energy metabolism[J].Chinese Journal of Tropical Crops, 2017, 38(1):175-182.
[5] 千春录, 殷建东, 王利斌, 等.1-甲基环丙烯和自发气调对猕猴桃品质及活性氧代谢的影响[J].食品科学, 2018, 39(11):233-240.
QIAN C L, YIN J D, WANG L B, et al.Effects of 1-methylcyclopropene treatment and self-developed modified atmosphere on quality and reactive oxygen species metabolism of kiwifruits during storage[J].Food Science, 2018, 39(11):233-240.
[6] SEREK M, PRABUCKI A, SISLER E, et al.Inhibitors of ethylene action final quality and rooting of cuttings before and after storage[J].Hortscience, 1998, 33(1):153-155.
[7] 李志文, 张平, 张昆明, 等.1-MCP结合冰温贮藏对葡萄果实质地的影响[J].农业机械学报, 2011, 42(7):176-181.
LI Z W, ZHANG P, ZHANG K M, et al.Effect of 1-methylcyclopropene combined with controlled freezing-point storage on texture of grape fruit[J].Transactions of the Chinese Society for Agricultural Machinery, 2011, 42(7):176-181.
[8] 唐欣影. ClO2缓释剂结合1-MCP 处理对西兰花常温货架期保鲜作用的研究[D].沈阳:沈阳农业大学, 2017.
TANG X Y.Study on the effects of sustained-release ClO2 and 1-MCP treatments on the quality of broccoli during shelf life at room temperature[D].Shenyang:Shenyang Agricultural University, 2017.
[9] 张礼良, 王馨悦, 姜爱丽, 等.氯化钙处理对采后蓝莓果实品质的影响[J].保鲜与加工, 2020, 20(4):1-8.
ZHANG L L, WANG X Y, JIANG A L, et al.Effect of calcium chloride treatment on quality of postharvest blueberries[J].Storage and Process, 2020, 20(4):1-8.
[10] 李艳娇, 朱璇, 敬媛媛, 等.NO与乙烯利处理对番茄果实采后软化相关酶活的影响[J].食品科技, 2016, 41(7):32-37.
LI Y J, ZHU X, JING Y Y, et al.Effects of the combination treatment of nitric oxide and ethephon on softening related enzyme activity of harvested tomato fruits[J].Food Science and Technology, 2016, 41(7):32-37.
[11] KHAN A S, SINGH Z.1-MCP regulates ethylene biosynthesis and fruit softening during ripening of ‘Tegan Blue’ plum[J].Postharvest Biogy and Technology, 2007, 43(3):298-306.
[12] 李江阔, 何宇光, 刘玲, 等.不同温度下1-MCP处理对枸杞鲜果贮藏品质的影响[J].包装工程, 2021, 42(15):10-18.
LI J K, HE Y G, LIU L, et al.Effects of 1-MCP treatment at different temperatures on storage quality of Lycium barbarum fruits[J].Packaging Engineering, 2021, 42(15):10-18.
[13] 何靖柳, 刘继, 黄彭, 等.几种保鲜处理对贮藏期间“红阳”猕猴桃抗氧化生理特性变化的影响[J].食品工业科技, 2014, 35(21):341-345;350.
HE J L, LIU J, HUANG P, et al.Effect on antioxidant activity of ‘Red Sun’ kiwifruit by several preservation treatments during postharvest period[J].Science and Technology of Food Industry, 2014, 35(21):341-345;350.
[14] 叶文斌, 贠汉泊, 樊亮, 等.复合涂膜对杨梅贮藏过程中POD、PPO、PAL酶活性的影响[J].包装与食品机械, 2012, 30(2):10-16.
YE W B, YUN H B, FAN L, et al.Effects of composite coating antistaling agent of polysaccharide treatment in storage on POD,PPO and PAL enzymatic activity of Chinese bayberry[J].Packaging and Food Machinery, 2012, 30(2):10-16.
[15] 秦晓杰, 肖红梅, 罗凯, 等.水杨酸结合拮抗酵母菌处理对冷藏草莓果实的抗性影响[J].食品科学, 2013, 34(18):290-294.
QIN X J, XIAO H M, LUO K, et al.Effect of antagonistic yeast in combination with salicylic acid on chilling resistance of strawberry fruits during cold storage[J].Food Science, 2013, 34(18):290-294.
[16] 陈嘉, 张立新, 冯志宏, 等.贮藏温度和 1-甲基环丙烯对四川青脆李褐变的影响[J].食品工业科技, 2014, 35(2):312-316;323.
CHEN J, ZHANG L X, FENG Z H, et al.Effects of storage temperature and 1-methylcyclopropene on browning of Sichuan Prunus americana[J].Science and Technology of Food Industry, 2014, 35(2):312-316;323.
[17] 韦波, 王爱勤.活性氧与植物系统获得抗性建立的信号导论的研究进展[C].广西植物生理学年会论文汇编.南宁:2003, 87-94.
WEI B, WANG A Q.Research progress of reactive oxygen species and signal establishment of plant system acquired resistance[C].Proceedings of Guangxi Annual Meeting of Plant Physiology.Nanning:2003, 87-94.
[18] JAMBUNATHAN N.Determination and detection of reactive oxygen species(ROS), lipid peroxidation,and electrolyte leakage in plants[J].Methods in Molecular Biology(Clifton,N.J.), 2010, 639:292-298.
[19] WANG Q, LAI T F, QIN G Z, et al.Response of jujube fruits to exogenous oxalic acid treatment based on Proteomic analysis[J].Plant and Cell Physiology, 2008, 50(2):230-242.
[20] 穆茜, 张丹丹, 杨祎凡, 等.1-MCP处理对“丽格”海棠果实采后生理及品质的影响[J].保鲜与加工, 2020, 20(1):18-23.
MU Q, ZHANG D D, YANG Y F, et al.Effects of 1-MCP treatment on postharvest physiology and quality of Rieger Begonia fruits[J].Storage and Process, 2020, 20(1):18-23.
[21] MINAS I S, CRISOSTO G M, HOLCROFT D, et al.Postharvest handling of plums(Prunus salicina Lindl.)at 10 ℃ to save energy and preserve fruit quality using an innovative application system of 1-MCP[J].Postharvest Biology and Technology, 2013, 76:1-9.
[22] SUPAPVANICH S, TUCKER G A.The effect of 1-MCP on quality and cell wall hydrolases activities of fresh-cut muskmelon (Cucumis melo var reticulatus L.) during storage[J].Food and Bioprocess Technology, 2013, 6(8):2 196-2 201.
[23] 杨玉荣, 赵晓敏, 李芸,等.1-MCP处理对采后库尔勒香梨货架期品质及生理的影响[J].食品科技, 2015, 40(5):37-41.
YANG Y R, ZHAO X M, LI Y,et al.Effect of 1-MCP treatment on qualities of Korla fragrant pear fruit during shelf-life[J].Food Science and Technology, 2015, 40(5):37-41.
[24] 张艳宜, 马婷, 宋小青, 等.1-MCP处理对猕猴桃货架期生理品质的影响[J].中国食品学报, 2014, 14(8):204-212.
ZHANG Y Y, MA T, SONG X Q, et al.Effects of 1-MCP treatment on physiological quality of kiwifruit during shelf-life[J].Journal of Chinese Institute of Food Science and Technology, 2014, 14(8):204-212.
[25] KHALIL M N A, FEKRY M I,FARAG M A.Farag.Metabolome based volatiles profiling in 13 date palm fruit varieties from Egypt via SPME GC-MS and chemometrics[J].Food Chemistry, 2017, 217:171-181.
文章导航

/