食品与发酵工业 >

2023 , Vol. 49 >Issue 6: 50 - 56

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.032036

研究报告

辐照裂褶菌多糖理化性质、体外消化模拟及益生菌发酵特性分析

  • 程雅清 ,
  • 殷朝敏 ,
  • 李雨鸿 ,
  • 范秀芝 ,
  • 姚芬 ,
  • 史德芳 ,
  • 高虹 ,
  • 胡国元
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  • 1(武汉工程大学 环境生态与生物工程学院, 绿色化工过程教育部重点实验室,湖北 武汉,430205)
    2(湖北省农业科学院农产品加工与核农技术研究所,湖北 武汉,430064)
    3(湖北省林下经济工程研究中心,湖北 武汉,430064)
程雅清(硕士研究生)和殷朝敏(博士,副研究员)为共同第一作者(高虹研究员和胡国元教授为共同通信作者,E-mail:highong@163.com;hgy701@163.com)

收稿日期: 2022-04-19

  修回日期: 2022-06-01

  网络出版日期: 2023-04-14

基金资助

国家自然科学基金项目(32072229; 31801921);湖北省农业科学院青年拔尖人才培养计划项目(2022);武汉市知识创新专项基础研究项目(2022020801010343);湖北省农业农村领域重点研发计划项目(2022BBA024)

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Physicochemical properties, in vitro digestion and probiotic fermentation characteristics of polysaccharides from γ-irradiated Schizophyllum commune

  • CHENG Yaqing ,
  • YIN Chaomin ,
  • LI Yuhong ,
  • FAN Xiuzhi ,
  • YAO Fen ,
  • SHI Defang ,
  • GAO Hong ,
  • HU Guoyuan
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  • 1(Key Laboratory for Green Chemical Process of Ministry of Education, School of Environmental Ecology and Biological Engineering, Wuhan Institute of Technology, Wuhan 430205, China)
    2(Institute of Agro-Products Processing and Nuclear-Agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan 430064, China)
    3(Research Center of Under-forest Economy in Hubei Province, Wuhan 430064, China)

Received date: 2022-04-19

  Revised date: 2022-06-01

  Online published: 2023-04-14

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摘要

为探究裂褶菌多糖的益生潜能,该实验以辐照裂褶菌多糖(γ-irradiated Schizophyllum commune, ISPG)为原料,探究了ISPG理化性质、体外肠道消化特性及其对鼠李糖乳杆菌和植物乳杆菌的增殖效果。结果显示ISPG总糖含量为81.84%质量分数,分子质量为17.68 kDa,单糖组成中葡萄糖含量最高。刚果红实验结果表明ISPG具有三螺旋结构,可能属于β型葡聚糖。抗氧化活性实验表明ISPG具有一定的抗氧化能力。ISPG在人工口腔液和胃肠消化液中的水解度较低,均小于6.5%;ISPG对鼠李糖乳杆菌和植物乳杆菌有一定的增殖效果,但不及低聚果糖增值效果。实验结果表明ISPG可作为一种潜在的新型益生元,为裂褶菌多糖新产品的开发提供参考。

关键词： 裂褶菌多糖; 水解度; 植物乳杆菌; 鼠李糖乳杆菌

本文引用格式

程雅清 , 殷朝敏 , 李雨鸿 , 范秀芝 , 姚芬 , 史德芳 , 高虹 , 胡国元 . 辐照裂褶菌多糖理化性质、体外消化模拟及益生菌发酵特性分析[J]. 食品与发酵工业, 2023 , 49(6) : 50 -56 . DOI: 10.13995/j.cnki.11-1802/ts.032036

Abstract

In order to explore the prebiotic potential of polysaccharides from γ-irradiated Schizophyllum commune (ISPG), the physical and chemical properties, in vitro digestion characteristics and proliferation effect on Lactobacillus rhamnosus and Lactobacillus plantarum of ISPG were studied in this work. The results showed that the total sugar content of ISPG was 81.84%. the molecular weight was 17.68 kDa, and glucose was the most abundant monosaccharide. The result of Congo red experiment showed that ISPG had triple helix structure, thus, ISPG might belong to β-glucan. The antioxidant activity experiment in vitro showed that ISPG had a certain antioxidant effect. Both the hydrolysis degree of ISPG in artificial oral fluid and gastrointestinal digestive fluid was less than 6.5%. Moreover, ISPG exerted a certain proliferation effect on L. rhamnosus and L. plantarum, but the proliferation effect was not as effective as FOS. The results showed that ISPG could be a potential new prebiotic and our research would serve as a reference for the product development of S. commune polysaccharides.

Key words： Schizophyllum commune polysaccharides; degree of hydrolysis; Lactobacillus plantarum; Lactobacillus rhamnosus

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