食品与发酵工业 >

2023 , Vol. 49 >Issue 6: 79 - 85

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.032048

研究报告

水芹多糖的提取及其对巨噬细胞RAW264.7免疫活性的初步研究

  • 程雷 ,
  • 崔明晓 ,
  • 刘可玉 ,
  • 刘克海
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  • 1(上海海洋大学 食品学院,上海,201306)
    2(上海水产品加工及贮藏工程技术研究中心,上海,201306)
硕士研究生(刘克海教授为通信作者,E-mail:khliu@shou.edu.cn)

收稿日期: 2022-04-19

  修回日期: 2022-06-04

  网络出版日期: 2023-04-14

基金资助

国家自然科学基金(81572989)

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Extraction of Oenanthe javanica polysaccharide and its immune activity of macrophage RAW264.7

  • CHENG Lei ,
  • CUI Mingxiao ,
  • LIU Keyu ,
  • LIU Kehai
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  • 1(College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China)
    2(Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, China)

Received date: 2022-04-19

  Revised date: 2022-06-04

  Online published: 2023-04-14

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摘要

多糖是水芹中重要功能性成分,其分离和活性的相关研究尚未报道。该研究基于超声波辅助法从水芹中成功分离获得功能性多糖成分——水芹多糖(Oenanthe javanica polysaccharide,OJP),利用响应面法优化了OJP的分离条件,并考察了OJP对巨噬细胞RAW264.7的激活作用。响应面优化结果显示,OJP最佳分离条件为超声温度83.6 ℃、超声时间51 min、料液比1∶29.7(g∶mL),提取率为13.81%。单糖组成结果显示OJP由阿拉伯糖(29.94%)、半乳糖(52.48%)、葡萄糖(11.32%)以及微量木糖(2.22%)、半乳糖醛酸(3.5%)和葡萄糖醛酸(0.53%)组成。细胞实验结果表明,OJP可促进RAW264.7巨噬细胞增殖及NO产生并增强RAW264.7细胞的吞噬能力。该研究为OJP的开发与应用提供了实验基础。

关键词： 水芹; 多糖; 响应面法; 单糖组成; 巨噬细胞RAW264.7

本文引用格式

程雷 , 崔明晓 , 刘可玉 , 刘克海 . 水芹多糖的提取及其对巨噬细胞RAW264.7免疫活性的初步研究[J]. 食品与发酵工业, 2023 , 49(6) : 79 -85 . DOI: 10.13995/j.cnki.11-1802/ts.032048

Abstract

Polysaccharide is an important functional component in Oenanthe javanica, and the research on the isolation and activity of this polysaccharide has not been reported. In this study, functional polysaccharide from O. javanica (OJP) were successfully isolated based on ultrasonic-assisted method. Isolation conditions of OJP were optimized using response surface methodology and the activation effects of OJP on macrophage RAW264.7 were investigated. Response surface optimization results showed that the optimal separation conditions of OJP were ultrasonic temperature 83.6 ℃, ultrasonic time 51 min, solid-liquid ratio 1∶29.7(g∶mL), and the extraction yield was 13.81%. Cell experiments showed that OJP could promote the proliferation and NO production of RAW264.7 macrophages and enhance the phagocytic ability of RAW264.7 cells. This study provided a research basis for the development and application of O. javanica polysaccharide.

Key words： Oenanthe javanica; polysaccharide; response surface methodology; monosaccharide composition; macrophage RAW264.7

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