To improve the fishy flavor of Carassius auratus gibelio, Carassius auratus gibelio was deodorized by Lactobacillus plantarum, yeast, and their combination, sensory evaluation, electronic nose combined with monolithic material sorptive extraction-gas chromatography-mass spectrometry were used to analyze the deodorization effect of different microbial fermentation on Carassius auratus gibelio and the changes of volatile flavor components. The effects of different biological deodorization treatments on the microstructure of Carassius auratus gibelio were analyzed by scanning electron microscope. Results showed that compared with the control without deodorization, the fishy substances such as hexanal, (E)-2-octenal, (E, E) -2,4-heptadienal, 1-octen-3-alcohol, and 2-ethylfuran were not detected in each treatment group, and the contents of octanal, decanal, and 1-penten-3-alcohol decreased significantly (P<0.05). At the same time, esters such as ethylacetate were produced in each treatment group, which gave fish a unique fat flavor and promoted the formation of good flavor. Different biological deodorization treatments had different effects on the microstructure of Carassius auratus gibelio. The effect of Lactobacillus plantarum treatment on the muscle fiber structure of crucian carp was relatively small. This treatment could keep the muscle fiber bundle structure intact and the texture clear. Comprehensive analysis showed that the Lactobacillus plantarum fermentation method was more suitable for Carassius auratus to deodorize, effectively improved the flavor, retained nutritional value, and had a certain commercial prospect.
[1] 王汉玲. 鲫鱼汤的加工工艺及风味物质研究 [D].南昌:江西科技师范大学, 2020.
WANG H L.Study on the processing technology and flavor substances of crucian carp soup[D].Nanchang:Jiangxi Science and Technology Normal University, 2020.
[2] 王方. 酸碱处理对鲫鱼糜腥味脱除效果及凝胶特性影响的研究 [D].武汉:华中农业大学, 2014.
WANG F.Effects of acid and alkali-aided processing on the removal of off-flavors and gel properties of surimi from crucian carp(Carastius auratus)[D].Wuhan:Huazhong Agricultural University, 2014.
[3] 付湘晋. 白鲢鱼脱腥及其低盐鱼糜制备的研究 [D].无锡:江南大学, 2009.
FU X J.Removing on the off-flavor of silver carp and the preparation of low salt surimi [D].Wuxi:Jiangnan University, 2009.
[4] 廖鄂. 淡水鱼发酵过程中N-亚硝胺的形成及控制机制研究 [D].无锡:江南大学, 2019.
LIAO E.Study on mechanism of N-nitrosamines formation and control during freshwater fish fermentation[D].Wuxi:Jiangnan University, 2019.
[5] 张晶晶, 王锡昌, 施文正.白姑鱼和小黄鱼肉中挥发性风味物质的鉴定 [J].食品科学, 2019, 40(14):206-213.
ZHANG J J, WANG X C, SHI W Z.Identification of volatile compounds in white croaker and small yellow croaker[J].Food Science, 2019, 40(14):206-213.
[6] ZHANG J J, WANG X C, SHI W Z.Odor characteristics of white croaker and small yellow croaker fish during refrigerated storage [J].Journal of Food Biochemistry, 2019, 43(10):e12852.
[7] 刘登勇, 周光宏, 徐幸莲.确定食品关键风味化合物的一种新方法:“ROAV”法[J].食品科学, 2008, 29(7):370-374.
LIU D Y, ZHOU G H, XU X L.“ROAV”method:A new method for determining key odor compounds of Rugao Ham[J].Food Science, 2008, 29(7):370-374.
[8] JIANG Q Q, NAKAZAWA N, HU Y Q, et al.Changes in quality properties and tissue histology of lightly salted tuna meat subjected to multiple freeze-thaw cycles [J].Food Chemistry, 2019, 293:178-186.
[9] UDOMSIL N, RODTONG S, CHOI Y J, et al. Use of Tetragenococcus halophilus as a starter culture for flavor improvement in fish sauce fermentation [J].Journal of Agricultural and Food Chemistry, 2011, 59(15):8 401-8 408.
[10] ZHENG B, LIU Y, HE X X, et al.Quality improvement on half-fin anchovy (Setipinnataty) fish sauce by Psychrobacter sp.SP-1 fermentation [J].Journal of the Science of Food and Agriculture, 2017, 97(13):4 484-4 493.
[11] 吴建中, 王倩倩, 廖顺, 等.安琪酵母发酵对秋刀鱼汤的脱腥作用原因分析[J].食品与发酵工业, 2019, 45(7):213-220.
WU J Z, WANG Q Q, LIAO S,et al.Causes analysis of deodorization of saury soup by angel yeast fermentation[J].Food and Fermentation Industries, 2019, 45(7):213-220.
[12] 袁晓阳,陆胜民,郁志芳.自然发酵腌制冬瓜主要发酵菌种及风味物质鉴定[J].中国食品学报,2009,9(1):219-225.
YUAN X Y, LU S M, YU Z F.Identification of main fermented strains and flavor substances in naturally fermented wax gourd with strong odour[J].Journal of Chinese Institute of Food Science and Technology, 2009,9(1):219-225.
[13] WU N, WANG X C, TAO N P, et al.Odor profiles of hepatopancreas and gonad of Eriocheir sinensis by sensory analysis, electronic nose, and GC-MS analysis [J].Fisheries Science, 2016, 82(3):537-547.
[14] 虎虓真. 基于多指纹图谱技术的中国赤霞珠葡萄酒产地溯源及年份识别 [D].上海:上海海洋大学, 2019.
HU X Z.Geographical origin, vintage discrimination of Chinese cabernet sauvignon wines based on multiple fingerprinting[D].Shanghai:Shanghai Ocean University, 2019.
[15] VARLET V, KNOCKAERT C, PROST C, et al.Comparison of odor-active volatile compounds of fresh and smoked salmon [J].Journal of Agricultural and Food Chemistry, 2006, 54 (9):3 391-3 401.
[16] 杨玉平, 熊光权, 程薇, 等.水产品异味物质形成机理、检测及去除技术研究进展 [J].食品科学, 2009, 30(23):533-538.
YANG Y P, XIONG G Q, CHENG W, et al.Review on formation mechanism, analysis and removal technologies of fishy off-odor[J].Food Science, 2009, 30(23):533-538.
[17] ROBIN J, CRAVEDI J P, HILLENWECK A, et al.Off flavor characterization and origin in French trout farming [J].Aquaculture, 2006, 260(1-4):128-138.
[18] JENSEN M T, HANSEN L L, ANDERSEN H J.Transfer of the meat aroma precursors (dimethyl sulfide, dimethyl disulfide and dimethyl trisulfide) from feed to cooked pork[J].LWT-Food Science and Technology, 2002, 35(6):485-489.
[19] 刘利平, 李慷, 闫莉.水产动物体内土腥味物质的来源、检测及其防控与去除的研究进展 [J].水产学报, 2021,45(5):813-829.
LIU L P, LI K, YAN L.Sources, determination, prevention and elimination of off-flavor compounds in aquatic animals [J].Journal of Fisheries of China, 2021,45(5):813-829.
[20] ROBERTSON R F, JAUNCEY K, BEVERIDGE M C M, et al.Depuration rates and the sensory threshold concentration of geosmin responsible for earthy-musty taint in rainbow trout, Onchorhynchus mykiss [J].Aquaculture, 2005, 245(1-4):89-99.
[21] MAHMOUD A A,BUETTNER A.Characterisation of aroma-active and off-odour compounds in German rainbow trout (Oncorhynchus mykiss).Part II:Case of fish meat and skin from earthen-ponds farming [J].Food Chemistry, 2017, 232:841-849.
[22] JOSEPHSON D B, LINDSAY R C, STUIBER D A.Biogenesis of lipid-derived volatile aroma compounds in the emerald shiner (Notropis atherinoides)[J].Journal of Agricultural and Food Chemistry, 1984, 32(6):1 347-1 352.
[23] THIANSILAKUL Y, BENJAKUL S, RICHARDS M P.Changes in heme proteins and lipids associated with off-odour of seabass (Latescalcarifer) and red tilapia (Oreochromis mossambicus×O.niloticus) during iced storage [J].Food Chemistry, 2010, 121(4):1 109-1 119.
[24] 王蔚新. 酸鱼发酵过程中蛋白质降解及其风味形成机制研究 [D].无锡:江南大学, 2017.
WANG W X.Research on proteolysis and flavor formation mechanism during suanyu fermentation [D].Wuxi:Jiangnan University, 2017.
[25] GARAVAGLIA J, HABEKOST A, BJERK T R, et al.A new method for rapid screening of ester-producing yeasts using in situ HS-SPME [J].Journal of Microbiological Methods, 2014, 103:1-2.
[26] GIRI A, OSAKO K, OHSHIMA T.Identification and characterisation of headspace volatiles of fish miso, a Japanese fish meat based fermented paste, with special emphasis on effect of fish species and meat washing [J].Food Chemistry, 2009, 120(2):621-631.
[27] CARDINAL M, CHAUSSY M, DONNAY-MORENO C, et al.Use of random forest methodology to link aroma profiles to volatile compounds:Application to enzymatic hydrolysis of Atlantic salmon (Salmo salar) by-products combined with Maillard reactions [J].Food Research International, 2020, 134:109254.
[28] GU S Q, WANG X C, TAO N P, et al.Characterization of volatile compounds in different edible parts of steamed Chinese mitten crab (Eriocheir sinensis)[J].Food Research International, 2013, 54(1):81-92.
[29] 孙颖瑛, 许艳顺, 夏文水, 等.发酵酸鱼贮藏过程中理化与感官品质变化研究 [J].食品工业科技, 2020, 41(17):286-291.
SUN Y Y, XU Y S, XIA W S, et al.Changes in physicochemical and sensory quality of fermented fish during storage [J].Science and Technology of Food Industry, 2020, 41(17):286-291.
[30] 韦诚. 发酵过程中酸肉蛋白质的变化及其对食用品质的影响 [D].重庆:西南大学, 2018.
WEI C.Changes of sour meat protein and its effect on edible quality during fermentation[D].Chongqing:Southwest University, 2018.