食品与发酵工业 >

2023 , Vol. 49 >Issue 6: 143 - 148

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.032471

研究报告

二肽AQ、SQ和IQ增强GES-1细胞对酒精损伤的抵抗能力

  • 李明亮 ,
  • 许锦珍 ,
  • 冯志远 ,
  • 李诒光 ,
  • 张卓然 ,
  • 刘丹 ,
  • 方磊 ,
  • 廖群 ,
  • 刘文颖 ,
  • 刘文君
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  • 1(中国食品发酵工业研究院有限公司, 北京市蛋白功能肽工程技术研究中心,北京,100015)
    2(江中药业股份有限公司,江西 南昌,330096)
硕士(刘文颖高级工程师和刘文君高级工程师为共同通信作者,E-mail:wenyingliu888@126.com;liuwenjun@crjz.com)

收稿日期: 2022-05-27

  修回日期: 2022-06-22

  网络出版日期: 2023-04-14

基金资助

宁夏回族自治区重点研发计划项目(2021BEG02027)

收起

Enhancement of dipeptides AQ, SQ, and IQ on the resistance of GES-1 cells to alcohol damage

  • LI Mingliang ,
  • XU Jinzhen ,
  • FENG Zhiyuan ,
  • LI Yiguang ,
  • ZHANG Zhuoran ,
  • LIU Dan ,
  • FANG Lei ,
  • LIAO Qun ,
  • LIU Wenying ,
  • LIU Wenjun
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  • 1(China National Research Institute of Food and Fermentation Industries Co.Ltd., Beijing Engineering Research Center of Protein and Functional Peptides, Beijing 100015,China)
    2(Jiangzhong Pharmaceutical Co.Ltd., Nanchang 330096, China)

Received date: 2022-05-27

  Revised date: 2022-06-22

  Online published: 2023-04-14

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摘要

部分食源性二肽具有良好的吸收特性以及生理活性,在功能食品的开发上具有强大的应用前景。该研究旨在阐明二肽AQ、SQ和IQ具有增强GES-1细胞对酒精损伤抵抗能力的作用。首先,该研究对GES-1细胞进行二肽预处理,之后,利用酒精建立细胞损伤模型;通过检测细胞存活率和细胞的乳酸脱氢酶释放量判断模型是否成立,通过检测细胞内ROS含量、抗氧化酶活力和氧化损伤标志物含量,评价二肽对细胞氧化应激的改善作用;通过检测细胞内紧密连接蛋白以及相关损伤标志物的蛋白表达水平,评价二肽对细胞屏障功能的增强作用。结果表明,上述3种二肽可以减轻细胞氧化应激,减少损伤标志物的表达水平并增加紧密连接蛋白的表达水平。该研究证明了上述3种二肽可以增强GES-1细胞抵抗酒精损伤的能力,在修复酒精性胃黏膜损伤,保护胃黏膜屏障功能方面具有一定的应用价值和潜力。

关键词： 二肽; GES-1细胞; 酒精损伤; 紧密连接蛋白; 胃黏膜屏障

本文引用格式

李明亮 , 许锦珍 , 冯志远 , 李诒光 , 张卓然 , 刘丹 , 方磊 , 廖群 , 刘文颖 , 刘文君 . 二肽AQ、SQ和IQ增强GES-1细胞对酒精损伤的抵抗能力[J]. 食品与发酵工业, 2023 , 49(6) : 143 -148 . DOI: 10.13995/j.cnki.11-1802/ts.032471

Abstract

Some food-derived dipeptides have good absorption properties as well as physiological activities, which have powerful application prospects in the development of functional foods. To elucidate the role of dipeptides AQ, SQ, and IQ in enhancing the resistance of GES-1 cells to alcohol damage. Firstly, GES-1 cells were pretreated with dipeptide for 24 h, after which a cell injury model was established using alcohol; whether the model was established by detecting cell survival rate and lactate dehydrogenase release from cells, and the ameliorative effect of dipeptide on cellular oxidative stress was evaluated by detecting intracellular ROS content, antioxidant enzyme activity and oxidative damage marker content. The repair effect of dipeptides on cellular tight junctions was evaluated by detecting the protein expression levels of intracellular tight junction proteins as well as related damage markers. The results showed that the dipeptide could reduce the oxidative stress condition of cells and the expression of damage markers, thus increase the expression level of tight junction proteins. This study demonstrated that the above-mentioned dipeptides can enhance the ability of GES-1 cells to resist alcohol damage, and have certain application value and potential in repairing alcoholic gastric mucosal damage and protecting gastric mucosal barrier function.

Key words： dipeptide; GES-1 cell; alcohol damage; tight junction protein; gastric mucosal barrier

参考文献

[1] ZHAO Z Y, GONG S L, WANG S M, et al.Effect and mechanism of evodiamine against ethanol-induced gastric ulcer in mice by suppressing Rho/NF-κB pathway [J].International Immunopharmacology, 2015, 28(1):588-595.
[2] 张雅博, 阮研硕, 赵晓红, 等.乙醇性胃粘膜损伤及其保护作用的研究进展 [J].食品安全质量检测学报, 2013, 4(4):1 239-1 245.
ZHANG Y B, RUAN Y S, ZHAO X H, et al.The research progress of gastric mucosal damage induced by ethanol and its protective effects [J].Journal of Food Safety and Quality, 2013, 4(4):1 239-1 245.
[3] 赵兰平, 张三明, 郑志新.多肽对小鼠酒精性胃粘膜损伤影响的观察 [J].张家口医学院学报, 2004(3):12-13.
ZHAO L P, ZHANG S M, ZHENG Z X.The effects of peptides on alchole gastric membrane hurt in mice [J].Journal of Zhangjiakou Medical College, 2004(3):12-13.
[4] ZHANG C, GAO F, GAN S, et al.Chemical characterization and gastroprotective effect of an isolated polysaccharide fraction from Bletilla striata against ethanol-induced acute gastric ulcer [J].Food and Chemical Toxicology: an International Journal Published for the British Industrial Biological Research Association, 2019, 131:110539.
[5] LIN K W, WANG Y, GONG J W, et al.Protective effects of total flavonoids from Alpinia officinarum rhizoma against ethanol-induced gastric ulcer in vivo and in vitro [J].Pharmaceutical Biology, 2020, 58(1):854-862.
[6] SANTOS S, TORCATO I, CASTANHO M A R B.Biomedical applications of dipeptides and tripeptides [J].Biopolymers, 2012, 98(4):288-293.
[7] ANO Y, YOSHINO Y, UCHIDA K, et al.Preventive effects of tryptophan-methionine dipeptide on neural inflammation and alzheimer's pathology[J].International Journal of Molecular Sciences, 2019, 20(13):3206.
[8] FREITAS A K L, SILVA M T B, SILVA C M S, et al.Alanyl-glutamine protects the intestinal barrier function in trained rats against the impact of acute exhaustive exercise [J].Brazilian Journal of Medical and Biological Research, 2020, 53(5):e9211.
[9] GUDASHEVA T A, BOYKO S S, AKPAROV V K, et al.Identification of a novel endogenous memory facilitating cyclic dipeptide cyclo-prolylglycine in rat brain [J].FEBS Letters, 1996, 391(1-2):149-152.
[10] HAN C H, LIN Y S, LIN S Y, et al.Antioxidant and antiglycation activities of the synthesised dipeptide, Asn-Trp, derived from computer-aided simulation of yam dioscorin hydrolysis and its analogue, Gln-Trp [J].Food Chemistry, 2014, 147:195-202.
[11] JIA Y Y, KIM J H, NAM B, et al.The dipeptide H-Trp-Arg-OH (WR) Is a PPAR α agonist and reduces hepatic lipid accumulation in lipid-loaded H4IIE cells [J].Applied Biochemistry and Biotechnology, 2015, 175(2):1 211-1 220.
[12] KANEGAWA N, SUZUKI C, OHINATA K.Dipeptide Tyr-Leu (YL) exhibits anxiolytic-like activity after oral administration via activating serotonin 5-HT1A, dopamine D1 and GABAA receptors in mice [J].FEBS Letters, 2010, 584(3):599-604.
[13] MIZUSHIGE T, UCHIDA T, OHINATA K.Dipeptide tyrosyl-leucine exhibits antidepressant-like activity in mice [J].Scientific Reports, 2020, 10(1):1-10.
[14] NAM S Y, KIM H M, JEONG H J.Anti-fatigue effect by active dipeptides of fermented porcine placenta through inhibiting the inflammatory and oxidative reactions [J].Biomedicine & Pharmacotherapy, 2016, 84:51-59.
[15] NITTA A, NISHIOKA H, FUKUMITSU H, et al.Hydrophobic dipeptide Leu-Ile protects against neuronal death by inducing brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor synthesis [J].Journal of Neuroscience Research, 2004, 78(2):250-258.
[16] SALE C, ARTIOLI G G, GUALANO B, et al.Carnosine:From exercise performance to health [J].Amino Acids, 2013, 44(6):1 477-1 491.
[17] SASAI M, SUN X H, OKUDA C, et al.Orally active anti-hypertensive peptides found based on enteroendocrine cell responses to a dipeptide library [J].Biochemical and Biophysical Research Communications, 2018, 503(2):1 070-1 074.
[18] XIE L, GUO Y L, CHEN Y R, et al.A potential drug combination of omeprazole and patchouli alcohol significantly normalizes oxidative stress and inflammatory responses against gastric ulcer in ethanol-induced rat model [J].International Immunopharmacology, 2020, 85:106660.
[19] 陈敏, 杜军, 李迎春, 等.慢性饮酒对大鼠胃粘膜氧自由基水平的影响 [J].右江医学, 2003, 31(5):417-419.
CHEN M, DU J, LI Y C, et al.The effect of chronic alcohol drinking to the gastric mucosal oxygen free radical levels in rats [J].Chinese Youjiang Medical Journal, 2003, 31(5):417-419.
[20] 冯琳, 常明, 唐年初.不同菌种发酵枸杞酵素对酒精性肝损伤的保护作用 [J].食品与发酵工业, 2021, 47(17):98-104.
FENG L, CHANG M, TANG N C.Protective effects on alcoholic liver injury by fermented Lycium barbarum juice[J].Food and Fermentation Industries, 2021, 47(17):98-104.
[21] WU H L, GAO X, JIANG Z D, et al.Attenuated expression of the tight junction proteins is involved in clopidogrel-induced gastric injury through p38 MAPK activation [J].Toxicology, 2013, 304:41-48.
[22] 吴海露, 张振玉, 段兆涛, 等.莫沙比利通过p38/MAPK通路保护氯吡格雷所致胃黏膜上皮细胞损伤[J].世界华人消化杂志, 2012, 20(36):3 632-3 637.
WU H L, ZHANG Z Y, DUAN Z T, et al. Mosapride protects against clopidogrel-induced gastric mucosal epithelium cell damage via the p38 MAPK signaling pathway [J].World Chinese Journal of Digestology, 2012, 20(36):3 632-3 637.
[23] DUAN J L, YIN J, REN W K, et al.Pyrrolidine dithiocarbamate restores gastric damages and suppressive autophagy induced by hydrogen peroxide [J].Free Radical Research, 2015, 49(2):210-218.
[24] 李春艳, 刘丽娜, 吕申, 等.一氧化氮、内皮素及氧自由基对大鼠酒精性胃损害的作用研究 [J].胃肠病学和肝病学杂志, 2000, 9(1):14-16.
LI C Y, LIU L N, LYU S, et al.Role of nitric oxide, endothelin and oxygen free radicals in ethanol- induced gastric mucosal injury in rats [J].Chinese Journal of Gastroenterology and Hepatology, 2000, 9(1):14-16.
[25] 李春艳, 刘丽娜, 吕申, 等.一氧化氮与氧自由基在大鼠急性乙醇性胃粘膜损伤中的作用 [J].中国病理生理杂志, 2004, 20(10):1 777-1 777;1 813.
LI C Y, LIU L N, LYU S, et al.Role of nitric oxide and oxygen radicals in acute ethanolic gastric mucosal injury in rats [J].Chinese Journal of Pathophysiology, 2004, 20(10):1 777-1 777;1 813.
[26] 苏红, 曹之宪, 林兆鑫.乙醇对大鼠胃粘膜的影响 [J].世界华人消化杂志, 2000, 8(3):355-356.
SU H, CAO Z X, LIN Z X.Effect of ethanol on the gastric mucosa of rats [J].World Chinese Journal of Digestology, 2000, 8(3):355-356.
[27] CHEN C, CAO M, WU D Y, et al.KH-type splicing regulatory protein mediate inflammatory response in gastric epithelial cells induced by lipopolysaccharide [J].Cell Biology International, 2017, 41(8):871-878.
[28] YANAKA A, SUZUKI H, SHIBAHARA T, et al.EGF promotes gastric mucosal restitution by activating Na+/H+ exchange of epithelial cells [J].American Journal of Physiology-Gastrointestinal and Liver Physiology, 2002, 282(5):G866-G876.
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