To reveal the protective effect of Bacillus subtilis JZXJ-7 (Bs-JZXJ-7) isolated from shrimp paste on histamine exposure in mice, the tolerance of Bs-JZXJ-7 to gastrointestinal juices was detected by in vitro digestion test. Subsequently, C57BL/6J mice were intra-gastrically treated with histamine (20 mg/kg BW) to construct a food poisoning model. The disease activity index (DAI) score, organ (heart, liver, spleen, lung, kidney, stomach and intestine) morphological and pathological changes, intestinal tight junction (TJ) protein (Zonula occluden-1 and Occludin) expression, cytokine (IL-1β, IL-6, IL-10 and tumor necrosis factor-α) levels and antioxidant (superoxide dismutase, catalase, total antioxidant capacity and malondialdehyde) activities in model mice were measured before and after exposure to Bs-JZXJ-7. In vitro studies showed that the Bs-JZXJ-7 survival rate remained high (91.53%) after incubating in gastrointestinal fluid, indicating that the strain was highly resistant to protease enzymolysis and acid/alkali digestion. Bs-JZXJ-7 significantly (P<0.05) reduced DAI score, alleviated splenic and intestinal edema, and minimized the pathological organ damage in mice. Also, Bs-JZXJ-7 significantly (P<0.05) up-regulated TJ protein expression, repaired intestinal barrier function, and inhibited colonic inflammation and oxidative stress. Overall, Bs-JZXJ-7 alleviated histamine toxicity damage and hence could be regarded as a “green” anti-histamine functional foods.
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