类干酪乳酪杆菌(Lacticaseibacillus paracasei)PC-01是1株分离自西藏拉萨地区自然发酵酸牦牛乳中并具有潜在益生特性的菌株。通过葡聚糖硫酸钠诱导小鼠结肠炎模型,测定试验期间小鼠存活率、体重、疾病活动指数评分、结肠长度、组织切片、血清中髓过氧化物酶及细胞因子浓度、粪便中乳酸及短链脂肪酸含量,评价了不同剂量类干酪乳酪杆菌PC-01对结肠炎恢复的促进作用。结果表明,高剂量的菌株PC-01(1×109 CFU/d)提高了小鼠的存活率,加快了小鼠体重的回升,明显改善了小鼠粪便形态及便血情况,促进小鼠结肠组织病变的恢复,减少了炎症范围,同时降低了血清中髓过氧化物酶及促炎因子IL-1β、IL-6的浓度,并显著提高了小鼠粪便中乳酸及短链脂肪酸的含量。当灌胃高剂量的菌株PC-01时,对小鼠结肠炎恢复的促进作用最为显著。
Lacticaseibacillus paracasei PC-01 is a potential probiotic candidate isolated from naturally fermented yak milk in Lhasa, Tibet, China. In this study, we investigated the effect of different doses of strain PC-01 on the recovery of dextran sulphate sodium-induced colitis in a mouse model. The survival rate, body weight, disease activity index score, colon length, tissue sections, serum concentrations of myeloperoxidase and cytokines, and fecal lactate and short-chain fatty acid contents of mice were measured during the test period, and the effects of different doses of strain PC-01 on the recovery of colitis were evaluated. The results showed that a high dose of strain PC-01(1×109 CFU/d) increased the survival rate, accelerated the body weight gain, significantly improved the fecal morphology and bloody stool, promoted the recovery of colonic tissue lesions in mice, reduced the extent of inflammation, lowered the serum concentrations of myeloperoxidase and pro-inflammatory factors IL-1β and IL-6, and significantly increased the fecal contents of lactic acid and short-chain fatty acids. Our data confirmed that a high- PC-01 dose had the most significant effect on the recovery of colitis in mice.
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