食品与发酵工业 >

2023 , Vol. 49 >Issue 8: 201 - 206

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.032345

研究报告

嗜酸乳杆菌NC55的安全性评价

  • 林赓 ,
  • 马欣 ,
  • 谢雨欣 ,
  • 王杰 ,
  • 谢欣茹 ,
  • 付加芳 ,
  • 曹广祥
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  • 1(山东第一医科大学(山东省医学科学院) 生物医学科学学院,山东 济南,250117)
    2(山东省医药生物技术研究中心,山东 济南,250117)
第一作者：硕士研究生(曹广祥研究员为通信作者,E-mail:caoguangxiang@sdfmu.edu.cn)

收稿日期: 2022-05-15

  修回日期: 2022-07-18

  网络出版日期: 2023-05-16

基金资助

山东省重点研发计划项目(2021ZDSYS27)

收起

Safety evaluation of Lactobacillus acidophilus NC55

  • LIN Geng ,
  • MA Xin ,
  • XIE Yuxin ,
  • WANG Jie ,
  • XIE Xinru ,
  • FU Jiafang ,
  • CAO Guangxiang
Expand
  • 1(School of Biomedical Sciences, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250117, China)
    2(Shandong Medicinal Biotechnology Center, Jinan 250117, China)

Received date: 2022-05-15

  Revised date: 2022-07-18

  Online published: 2023-05-16

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摘要

嗜酸乳杆菌(Lactobacillus acidophilus)广泛应用于发酵饮品及食品领域。该实验室从发酵食品中分离获得一株嗜酸乳杆菌,将其命名为嗜酸乳杆菌NC55,该研究通过抗生素敏感实验、有害代谢产物实验及动物实验对NC55菌株进行安全性评价。结果显示,NC55菌株对多数抗生素敏感,未携带质粒,不存在耐药性通过质粒传递的可能。NC55菌株无氨基脱羧酶活性,菌株表现为不溶血。动物实验过程中小鼠生命活动正常,解剖发现主要脏器无病变,未发现细菌移位现象;致畸试验显示小鼠胚胎发育正常。综上,嗜酸乳杆菌NC55在抗生素敏感实验、质粒检测、溶血实验及动物实验中均表现出良好的安全性,可被认为是应用于人和动物的潜在有益菌株。

关键词： 嗜酸乳杆菌; 安全性评价; 抗生素敏感实验; 溶血实验; 动物实验

本文引用格式

林赓 , 马欣 , 谢雨欣 , 王杰 , 谢欣茹 , 付加芳 , 曹广祥 . 嗜酸乳杆菌NC55的安全性评价[J]. 食品与发酵工业, 2023 , 49(8) : 201 -206 . DOI: 10.13995/j.cnki.11-1802/ts.032345

Abstract

Lactobacillus acidophilus has been widely used in fermented drinks and foods. Strain NC55 was previously isolated from fermented food and identified as Lactobacillus acidophilus. Antibiotic sensitivity test, harmful metabolite test and animal experiment were performed to evaluate safety of NC55. Results showed that NC55 was sensitive to most of the antibiotics tested; and no plasmid was detected in NC55, indicating that NC55 could not transfer antibiotic resistant genes through plasmid. No amino decarboxylase activity and hemolytic activity were found in NC55. Animal experimental results showed that the life activities of the mice were normal, and there were no bacterial translocation or pathological changes in the main organs. Teratogenic test showed that mouse embryos developed normal. In conclusion, strain NC55 showed high safety, It can be considered as a potentially beneficial strain used in humans and animals.

Key words： Lactobacillus acidophilus; safety evaluation; antibiotic susceptibility test; hemolysis test; animal experiment

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