研究报告

灵芝孢子粉微胶囊的制备及其氧化稳定性研究

  • 祝敏珉 ,
  • 雷俊 ,
  • 陈俊滔 ,
  • 贺丹 ,
  • 何荣军 ,
  • 孙培龙
展开
  • 1(浙江工业大学 食品科学与工程学院,浙江 杭州,310020)
    2(浙江科达生物科技有限公司,浙江 丽水,323000)
硕士研究生(何荣军副教授为通信作者,E-mail:hrjun@zjut.edu.cn)

收稿日期: 2022-03-09

  修回日期: 2022-04-01

  网络出版日期: 2023-06-05

基金资助

浙江省龙泉市科技计划项目(2020KJ-001)

Preparation and oxidative stability of Ganoderma lucidum spore microcapsules

  • ZHU Minmin ,
  • LEI Jun ,
  • CHEN Juntao ,
  • HE Dan ,
  • HE Rongjun ,
  • SUN Peilong
Expand
  • 1(Collogy of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310020, China)
    2(Zhejiang Keda biological technology Co.Ltd., Lishui 323000, China)

Received date: 2022-03-09

  Revised date: 2022-04-01

  Online published: 2023-06-05

摘要

灵芝孢子粉具有多种生理活性,但其存在易氧化等缺点。该文采用复凝聚法制备银耳原胶-明胶复配壁材的灵芝孢子粉微胶囊,并分析了其氧化稳定性。通过响应面实验优化后的最佳制备工艺为:破壁灵芝孢子粉质量分数33.1%、银耳原胶质量分数36.5%、明胶质量分数30.4%、包埋pH 4.1时的包埋率为94.0%。灵芝孢子粉微胶囊的微观结构呈现不规则皱缩的球形,囊壁完整性好。在加速氧化实验中,以油脂过氧化值、酸价作为评价指标,灵芝孢子粉微胶囊相比于未包埋的灵芝破壁孢子粉具有更好的氧化稳定性。微胶囊化有助于提高破壁灵芝孢子粉功能性物质的氧化稳定性,扩大其在食品工业中的应用范围。

本文引用格式

祝敏珉 , 雷俊 , 陈俊滔 , 贺丹 , 何荣军 , 孙培龙 . 灵芝孢子粉微胶囊的制备及其氧化稳定性研究[J]. 食品与发酵工业, 2023 , 49(9) : 207 -211 . DOI: 10.13995/j.cnki.11-1802/ts.031364

Abstract

Ganoderma lucidum spore is a healthy food with physiological activities, but it is easily oxidized. In this paper, G. lucidum spore microcapsules were prepared by complex coagulation method with Tremella fuciformis colloid-gelatin composite as wall material. Its oxidation stability was also analyzed. The preparation process optimized by response surface experiment was as follows: the mass fraction of broken G. lucidum spore was 33.1%, the mass fraction of T. fuciformis colloid was 36.5%, the mass fraction of gelatin was 30.4%, and the embedded pH was 4.1. In these conditions, the embedding rate was 94.0%. The surface of the G. lucidum spore microcapsule was irregular and shrunk spherical, and the integrity of the capsule wall was great. In the accelerated oxidation experiment, the G. lucidum spore microcapsule had better oxidation stability compared with the nonembedded G. lucidum wall-broken spore powder. In conclusion, microencapsulation of G. lucidum spore can improve oxidation stability and has application potential in the food industry.

参考文献

[1] SHI Y J, ZHENG H X, HONG Z P, et al.Antitumor effects of different Ganoderma lucidum spore powder in cell- and zebrafish-based bioassays[J].Journal of Integrative Medicine, 2021, 19(2):177-184.
[2] 刘洋, 覃媚媚, 罗印斌, 等.灵芝孢子主要活性成分及其功能研究进展[J].北方园艺, 2020(5):138-146.
LIU Y, QIN M M, LUO Y B, et al.Research progress on component and function of Ganoderma lucidium spore[J].Northern Horticulture, 2020(5):138-146.
[3] CAI M, MU H, XING H Y, et al.In vitro gastrointestinal digestion and fermentation properties of Ganoderma lucidum spore powders and their extracts[J].LWT, 2021, 135:110235.
[4] 杨晓青, 王旭.灵芝孢子粉最新应用研究概况[J].江苏中医药, 2018, 50(4):87-89.
YANG X Q, WANG X.The latest application and research survey of Ganoderma spores powder[J].Jiangsu Journal of Traditional Chinese Medicine, 2018, 50(4):87-89.
[5] 陈慧, 王晓玲, 刘高强.灵芝子实体中三萜酸的提取及其稳定性[J].食用菌学报, 2015, 22(4):58-64.
CHEN H, WANG X L, LIU G Q.Optimization of triterpenoid acid extraction from Ganoderma lingzhi fruit bodies and stability under different temperature-time regimes[J].Acta Edulis Fungi, 2015, 22(4):58-64.
[6] LIANG C Y, TIAN D N, LIU Y Z, et al.Review of the molecular mechanisms of Ganoderma lucidum triterpenoids:Ganoderic acids A, C2, D, F, DM, X and Y[J].European Journal of Medicinal Chemistry, 2019, 174:130-141.
[7] ZHOU D, ZHOU F X, MA J F, et al.Microcapsulation of Ganoderma lucidum spores oil:Evaluation of its fatty acids composition and enhancement of oxidative stability[J].Industrial Crops and Products, 2019, 131:1-7.
[8] LI F F, WANG H F, MEI X H.Preparation and characterization of phytosterol-loaded microcapsules based on the complex coacervation[J].Journal of Food Engineering, 2021, 311:110728.
[9] 黎晨晨, 马永强, 井弘书, 等.新型纳米递送系统研究动态及在食品中的应用进展[J].中国食品添加剂, 2021, 32(5):126-131.
LI C C, MA Y Q, JING H S, et al.Research trends on application and development of new nanodelivery systems in food industry[J].China Food Additives, 2021, 32(5):126-131.
[10] 胡佳慧, 刘贵珍, 郝丽, 等.复凝聚法制备槐米芦丁微胶囊工艺优化[J].食品科技, 2021, 46(12):237-244.
HU J H, LIU G Z, HAO L, et al.Optimization of preparation technology of microcapsulation of rutin from Sophora japonicum by complex coacervation[J].Food Science and Technology, 2021, 46(12):237-244.
[11] ZHOU D, PAN Y, YE J, et al.Preparation of walnut oil microcapsules employing soybean protein isolate and maltodextrin with enhanced oxidation stability of walnut oil[J].LWT-Food Science and Technology, 2017, 83:292-297.
[12] 孙雪, 张蕊, 范方宇, 等.复凝聚法制备西番莲果皮花色苷微胶囊及其性质分析[J].食品工业科技, 2021, 42(17):187-193.
SUN X, ZHANG R, FAN F Y, et al.Preparation and properties of anthocyanin microcapsules from Passiflora edulis rind by complex coacervation[J].Science and Technology of Food Industry, 2021, 42(17):187-193.
[13] 李芳, 陈艳君, 高雷雷, 等.灵芝孢子油微囊的制备工艺研究[J].广州化工, 2021, 49(15):104-107.
LI F, CHEN Y J, GAO L L, et al.Study on preparation process of Ganoderma lucidum spore oil microcapsules[J].Guangzhou Chemical Industry, 2021, 49(15):104-107.
[14] NIU B, FENG S M, XUAN S Q, et al.Moisture and caking resistant Tremella fuciformis polysaccharides microcapsules with hypoglycemic activity[J].Food Research International, 2021, 146:110420.
[15] 陈婵, 黄靖, 陈晓波, 等.超声波提取银耳蒂头粗多糖工艺的优化研究[J].农产品加工, 2019(24):43-46.
CHEN C, HUANG J, CHEN X B, et al.Study on ultrasonicly extracting technology of polysaccharide from Tremella stalk[J].Farm Products Processing, 2019(24):43-46.
[16] 吴琴, 陶瑞霄, 迟原龙, 等.银耳粗多糖的理化特性研究[J].食品科技, 2016, 41(12):149-153.
WU Q, TAO R X, CHI Y L, et al.Physicochemical properties of crude polysaccharides from Tremella fucitormis[J].Food Science and Technology, 2016, 41(12):149-153.
[17] 刘维, 虎虓真, 朱莉, 等.灵芝三萜的研究与应用进展[J].食品科学, 2019, 40(5):309-315.
LIU W, HU X Z, ZHU L, et al.Recent progress in research and application of Ganoderma lucidum triterpenoids[J].Food Science, 2019, 40(5):309-315.
[18] 张汉辉, 程杏安, 李俊杰, 等.荔枝多酚微胶囊的制备工艺优化及其特性分析[J].食品工业科技, 2021, 42(23):201-208.
ZHANG H H, CHEN X A, LI J J, et al.Optimization of preparation process of lichi polyphenols microcapsules and its characteristic analysis[J].Science and Technology of Food Industry, 2021, 42(23):201-208.
[19] BILGIC A, CIMEN A, BASTUG E, et al.Fluorescent sporopollenin microcapsule modified by BODIPY for sensitive&selective recognition and efficient removal of Cu (II) from aqueous solution[J].Chemical Engineering Research and Design, 2022, 178:61-72.
[20] 杨颖, 潘兆平, 李绮丽, 等.响应面法优化赣南脐橙全果果酱微波制作工艺[J].中国食品学报, 2020, 20(12):167-175.
YANG Y, PAN Z P, LI Q L, et al.Optimization of microwave production process of Gannan navel orange whole fruit jam by response surface methodology[J].Journal of Chinese Institute of Food Science and Technology, 2020, 20(12):167-175.
[21] 刘青梅, 高娟, 姜敏芳, 等.响应面法优化杨梅渣中花色苷提取工艺[J].中国食品学报, 2014, 14(6):90-96.
LIU Q M, GAO J, JIANG M F, et al.Optimization of extraction technique of anthocyanins from red bayberry by response surface methodology[J].Journal of Chinese Institute of Food Science and Technology, 2014, 14(6):90-96.
[22] 王民申, 李勇, 冯进, 等.响应面法优化黄浆水絮凝工艺及其代谢组学分析[J].食品工业科技, 2022, 43(1):244-252.
WANG M S, LI Y, FENG J, et al.Optimization of flocculation process of yellow serofluid by response surface methodology and its metabolomics analysis[J].Science and Technology of Food Industry, 2022, 43(1):244-252.
[23] CUI N N, WANG G X, MA Q H, et al.Evolution of lipid characteristics and minor compounds in hazelnut oil based on partial least squares regression during accelerated oxidation process[J].LWT, 2021, 150:112025.
[24] 曹文明, 薛斌, 屠炳芳.酸价的特异性检测方法:铜皂络合比色技术的研究进展[J].中国油脂, 2022, 47(3):110-116.
CAO W M, XUE B, TU B F.Progress on cupric acetate-pyridine colorimetric method for specific quantitative determination of acid value[J].China Oils and Fats, 2022, 47(3):110-116.
文章导航

/