为提高发酵法生产核黄素的产量,解决发酵培养基中原料消耗高,发酵时间长等问题,该研究对枯草芽孢杆菌生产核黄素的培养基进行了优化。通过单因素试验、正交试验、5 L发酵罐过程控制,以菌体生物量、核黄素产量、糖酸转化率及副产物有机酸含量为指标,确定了发酵生产核黄素金属离子及生长因子的添加量,即:CuSO4·5H2O 4 g/L,FeSO4·7H2O 10.2 g/L,ZnSO4 6.2 g/L,磷酸吡哆醛(pyridoxal phosphate,PLP)4.2 g/L和维生素H 6.2 g/L,同时确定使用持续流加生长因子的策略。最终实验组菌体生物量(OD600值)达到了256.1,较未优化前提高了29.1%,核黄素最终产量为23.1 g/L,较未优化前提高了53.0%,糖酸转化率达到12%,同时减少了有机酸的生成量。研究结果显示,优化后的培养基显著提高了核黄素的产量以及糖酸转换率,实现了降本增效的目的。
In order to improve the yield of riboflavin by fermentation, solve the problems of high consumption of raw materials in fermentation medium and long fermentation time, medium for riboflavin production by Bacillus subtilis was further optimized. riboflavin yield, conversion of glycolic acid and by-product organic acid content, as indicators, the added amount of riboflavin metal ions and growth factors in fermentation production were determined by single factor experiments, orthogonal experiments, 5 L fermenter process control, biomass, namely: CuSO4·5H2O 4 g/L, FeSO4·7H2O 10.2 g/L, ZnSO4 6.2 g/L, PLP 4 2 g/L and vitamin H 6.2 g/L. while a strategy of continuous flow with growth factors was determined. The final bacterial biomass reached 256.1, an increase of 29.1% compared to the unoptimized group, with a final production of 23.1 g/L of riboflavin, an increase of 53.0% compared to the unoptimized group, a 12% conversion of glycolic acid and a reduction in the production of organic acids. The results of the study showed that the optimized medium significantly improved the production of riboflavin and the conversion rate of glycolic acid, thus achieving cost reduction and efficiency.
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