以植物乳植杆菌AR113为供试菌株,利用在线工具dbCAN,根据Diamond、HMMER以及Hotpep模型预测AR113的碳水化合物酶类(carbohydrate-active enzyme,CAZyme),并以光密度值(OD600)及最大比生长速率作为指标,验证植物乳植杆菌AR113对多种碳源的利用情况。结果表明,AR113中CAZymes按功能分为有6大类,分别为糖苷水解酶家族(GHs)基因54个、糖基转移酶家族(GTs)基因37个、碳水化合物结合模块(CBMs)基因6个、碳水化合物酯酶家族(CEs)基因2个以及1个辅助活性酶家族(AA)基因。根据生长实验发现,AR113可利用葡萄糖、麦芽糖、纤维二糖、甘露糖、低聚果糖、果糖、蔗糖、低聚半乳糖、甘露醇、山梨醇、低聚木糖、乳糖,但不可利用阿拉伯糖、肌醇,与碳水化合物酶预测结果一致,说明AR113可利用的碳源范围非常广泛。并且从碳源利用情况看,AR113培养基最适碳源为麦芽糖,MRS培养基相较于CDM培养基更有利于AR113生长。该研究解析了植物乳植杆菌AR113可利用的碳源谱,同时为植物乳植杆菌培养基的碳源选择和益生元的开发提供了理论依据。
Using Lactiplantibacillus plantarum AR113 as the test strain, the online tool dbCAN was used to predict the carbohydrate enzyme class (CAZyme) of AR113 according to Diamond, HMMER and Hotpep models, and the optical density value (OD600) and the maximum specific growth rate were used as indicators to verify the utilization of multiple carbon sources by AR113. The results showed that there were six major categories of CAZymes in AR113, including 54 genes of glycoside hydrolase family (GHs), 37 genes of glycosyltransferase family (GTs), six genes of carbohydrate-binding modules (CBMs), two genes of carbohydrate esterase family (CEs) and one gene of auxiliary active enzyme family (AA). Consistent with carbohydrate enzyme predictions, the experiments showed that AR113 can utilize glucose, maltose, cellobiose, mannose, oligofructose, fructose, sucrose, oligogalactose, mannitol, sorbitol, oligosaccharide, and lactose, but not arabinose and inositol, indicating that AR113 can utilize a very wide range of carbon sources. The most suitable carbon source for AR113 was maltose, and the growth of AR113 was more favorable in MRS medium compared to CDM medium. In this study, the carbon source spectrum available for L. plantarum AR113 was analyzed, and a theoretical basis was provided for the carbon source selection and prebiotic development of L. plantarum medium.
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