食品与发酵工业 >

2023 , Vol. 49 >Issue 12: 54 - 61

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.032864

研究报告

山楂多糖的分离纯化及其对粪便菌群的影响

  • 廖强 ,
  • 邓志扬 ,
  • 刘佳 ,
  • 张文静 ,
  • 邵淑娟 ,
  • 吴姗 ,
  • 刘军
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  • 1(中国农业大学 食品科学与营养工程学院,北京,100083)
    2(菏泽市食品药品检验检测研究院,山东 菏泽,274000)
    3(西安银桥乳业(集团)有限公司,陕西 西安,710075)
硕士研究生(刘军副教授为通信作者,E-mail:junliu@cau.edu.cn)

收稿日期: 2022-07-06

  修回日期: 2022-08-16

  网络出版日期: 2023-07-13

基金资助

国家自然科学基金青年基金项目(31701549)

收起

Isolation and purification of polysaccharides from hawthorn fruit and the effects on fecal microbiota

  • LIAO Qiang ,
  • DENG Zhiyang ,
  • LIU Jia ,
  • ZHANG Wenjing ,
  • SHAO Shujuan ,
  • WU Shan ,
  • LIU Jun
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  • 1(College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China)
    2(Heze City of Food and Drug Inspection and Testing Institute, Heze 274000, China)
    3(Xi’an Yinqiao Dairy (Group) Co.Ltd., Xi’an 710075, China)

Received date: 2022-07-06

  Revised date: 2022-08-16

  Online published: 2023-07-13

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摘要

多糖是山楂中重要功能活性成分,具有免疫调节、抗炎、调节糖脂代谢、抗糖基化等多种有益于人体健康的功能活性。该研究采用水提醇沉法从新鲜山楂中提取多糖,经大孔吸附树脂AB-8脱色后先后通过Q-sepharoseTM Fast Flow阴离子交换树脂及SephadexTM G-500凝胶对山楂多糖进行分离纯化,收集得到组分HFP-2并对其单糖组成及结构特征进行分析。结果表明,HFP-2的总糖与蛋白质含量分别为(93.84±0.58)%和(5.41±0.05)%,其分子质量为2.60×105 kDa,主要由半乳糖醛酸(36.91 mol%)、阿拉伯糖(28.11 mol%)、葡萄糖(15.02 mol%)、半乳糖(13.06 mol%)、鼠李糖(7.49 mol%)构成。结合傅里叶变换红外光谱分析HFP-2结构中官能团推测该组分为类似半乳糖醛酸为主链的果胶结构。体外粪便菌群发酵实验表明,HFP-2能够显著增加Phascolarctobacterium、Bacteroides、Prevotella、Roseburia等菌属的相对丰度(P<0.05)且促进乙酸、丙酸、异丁酸、丁酸等短链脂肪酸的生成。HFP-2能够通过调节肠道菌群多样性及代谢发挥益生作用,可作为益生元应用于功能食品。

关键词： 山楂多糖; 分离纯化; 体外发酵; 短链脂肪酸; 粪便菌群

本文引用格式

廖强 , 邓志扬 , 刘佳 , 张文静 , 邵淑娟 , 吴姗 , 刘军 . 山楂多糖的分离纯化及其对粪便菌群的影响[J]. 食品与发酵工业, 2023 , 49(12) : 54 -61 . DOI: 10.13995/j.cnki.11-1802/ts.032864

Abstract

Polysaccharides are important functional components in hawthorn fruit with health beneficial effects of immunomodulatory, anti-inflammation, regulation on glucose and lipid metabolism, and anti-glycation activities.In this study, polysaccharides were aqueous extracted from fresh hawthorn fruit and collected by alcoholic precipitation.Then, the collected polysaccharide fractions were subjected for decolorization using macroporous adsorption resin AB-8, and subsequently separated by Q-SepharoseTM Fast Flow anion exchange chromatography and SephadexTM G-500 gel chromatography.A homogenous polysaccharide fraction that denoted as HFP-2 was obtained and its chemical composition and structural features were further characterized.The total sugar and protein content of HFP-2 was (93.84±0.58)% and (5.41±0.05)%, respectively.HFP-2 was majorly composed by galacturonic acid (36.91 mol%), arabinose (28.11 mol%), glucose (15.02 mol%), galactose (13.06 mol%), and rhamnose (7.49 mol%), with an averaged molecular weight of 2.60×105 kDa.Combined with the identified functional groups by Fourier transform infrared spectroscopy, HFP-2 could have a structure that similar with pectin with backbone of homogalacturonan.The prebiotic activity of HFP-2 was analyzed by in vitro fermentation with fecal microbiota.After 48 h fermentation, HFP-2 significantly (P<0.05) increased the relative abundance of genera Phascolarctobacterium, Bacteroides, Prevotella, and Roseburia.Moreover, the production of short chain fatty acids such as acetate, propionate, isobutyrate, and butyrate was significant increased after fecal microbial fermentation of HFP-2.Findings gained in this study indicated that HFP-2 exerted prebiotic effects through altered composition and metabolization of intestinal flora.Thus, HFP-2 could be potentially used as prebiotics in functional foods.

Key words： hawthorn fruit polysaccharides; isolation and purification; in vitro fermentation; short chain fatty acids; fecal microbiota

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