食品与发酵工业 >

2023 , Vol. 49 >Issue 12: 106 - 112

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.035128

研究报告

鲍鱼肌肉酶解产物对秀丽隐杆线虫的抗衰老作用研究

  • 蔡佳琦 ,
  • 秦小明 ,
  • 卢虹玉 ,
  • 叶琬祺 ,
  • 张桂芳 ,
  • 林海生 ,
  • 郑惠娜
展开
  • 1(广东海洋大学 食品科技学院,广东省水产品加工与安全重点实验室,广东省海洋生物制品工程实验室,广东省海洋食品工程技术研究中心,水产品深加工广东普通高等学校重点实验室,广东 湛江,524088)
    2(海洋食品精深加工关键技术省部共建协同创新中心,大连工业大学,辽宁 大连,116034)
硕士研究生(秦小明教授和卢虹玉副教授为共同通信作者,E-mail:qinxm@gdou.edu.cn;irislhy@126.com)

收稿日期: 2023-02-14

  修回日期: 2023-03-15

  网络出版日期: 2023-07-13

基金资助

财政部和农业农村部国家现代农业产业技术体系资助(CARS-49)

收起

Anti-aging effect of Abalone muscle enzymatic hydrolysate on Caenorhabditis elegans

  • CAI Jiaqi ,
  • QIN Xiaoming ,
  • LU Hongyu ,
  • YE Wanqi ,
  • ZHANG Guifang ,
  • LIN Haisheng ,
  • ZHENG Huina
Expand
  • 1(College of Food Science and Technology, Guangdong Ocean University, National Research and Development Branch Center for Shellfish Processing (Zhanjiang), Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, Zhanjiang 524088, China)
    2(Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University,Dalian 116034, China)

Received date: 2023-02-14

  Revised date: 2023-03-15

  Online published: 2023-07-13

Fold

摘要

该研究以鲍鱼为原料,制备鲍鱼肌肉酶解产物(Abalone muscle water extract residue enzymatic product,ARE)并进行成分分析。通过对秀丽隐杆线虫寿命、运动能力、体内脂褐素水平、繁殖能力、抗应激能力等的分析测定,评价ARE抗衰老活性。结果表明,ARE主要成分为蛋白质;与对照组相比,高中低剂量的ARE均能显著延长秀丽隐杆线虫的寿命、提高线虫的运动能力(A型运动比例增加)、降低体内脂褐素水平,其中最高剂量ARE处理组(2.50 mg/mL)的作用最强,上述指标水平分别改变了17.36%、56.66%和21.93%(P＜0.05)。ARE能够提高线虫的抗应激能力,与对照组相比,2.50 mg/mL ARE处理组能够提高线虫热应激损伤存活率35.85%,增加了百草枯氧化应激损伤线虫的平均寿命25.73%。ARE提高了线虫体内抗氧化酶超氧化物岐化酶和谷胱甘肽的水平,显著降低了线虫体内丙二醛含量。另外,ARE在延长寿命的同时提高了繁殖能力。这些结果表明ARE具有较好的抗衰老活性,为开发鲍鱼功能性食品奠定了良好的基础。

关键词： 鲍鱼; 酶解产物; 秀丽隐杆线虫; 抗衰老; 氧化应激

本文引用格式

蔡佳琦 , 秦小明 , 卢虹玉 , 叶琬祺 , 张桂芳 , 林海生 , 郑惠娜 . 鲍鱼肌肉酶解产物对秀丽隐杆线虫的抗衰老作用研究[J]. 食品与发酵工业, 2023 , 49(12) : 106 -112 . DOI: 10.13995/j.cnki.11-1802/ts.035128

Abstract

In present study, the Abalone muscle water extract residue enzymatic product (ARE) was prepared and analyzed.To evaluate the anti-aging activity of ARE, longevity, exercise ability, lipofuscin level, reproductive ability, and anti-stress ability of Caenorhabditis elegans were detected respectively.The results showed that the main component of ARE was protein.Compared with the control group, the high, middle, and low dose of ARE could significantly prolong the life span of C.elegans, improve the movement ability of C.elegans (the proportion of type A movement increased), and reduce the level of lipofuscin in vivo.Among them, the highest dose of ARE treatment group (2.50 mg/mL) had the strongest effect.The levels of the above indicators changed by 17.36%, 56.66%, and 21.93% respectively (P<0.05).ARE improved the anti-stress ability of C.elegans.Specifically, the 2.5 mg/mL ARE treatment group compared with the control group improved the survival rate of heat-damaged C.elegans by 35.85%, and increased the average life span of C.elegans by 25.73% under paraquat induced stress condition.Meanwhile, ARE increased the levels of superoxide dismutase (SOD) and glutathione (GSH) in C.elegans and significantly reduced malondialdehyde (MDA) level.In addition, ARE increased reproductive capacity accompanied with prolonged life span of C.elegans.In conclusion, ARE exhibits good anti-aging activity, which lays a good foundation for the development of abalone functional food.

Key words： Abalone; enzymatic hydrolysate; Caenorhabditis elegans; anti-aging; oxidative stress

参考文献

[1] 王丹,吴反修.中国渔业统计年鉴[M].北京:中国农业出版社,2022.
WANG D, WU F X.China Fishery Statistical Yearbook[M].Beijing:China Agriculture Press, 2022.
[2] SHI L F, HAO G X, CHEN J, et al.Nutritional evaluation of Japanese abalone (Haliotis discus hannai Ino) muscle:Mineral content, amino acid profile and protein digestibility[J].Food Research International, 2020,129:108876.
[3] 刘先进, 陈胜军, 李来好, 等.四种鲍鱼肌肉营养成分分析与品质评价[J].食品与发酵工业, 2018,44(5):227-231.
LIU X J, CHEN S J, LI L H, et al.Nutritional analysis and quality evaluation of four kinds of abalone muscle[J].Food and Fermentation Industries, 2018,44(5):227-231.
[4] 王敏, 卢赛, 张曾亮, 等.鲍鱼水解肽的抗氧化、抗炎及免疫调节作用[J].食品工业科技, 2021,42(5):282-288.
WANG M, LU S, ZHANG Z L, et al.Antioxidant, anti-inflammatory and immunomodulatory effects of abalone hydrolytic peptide[J].Science and Technology of Food Industry, 2021,42(5):282-288.
[5] ZHENG P S, HAO G X, WENG W Y, et al.Antioxidant activities of hydrolysates from abalone viscera using subcritical water-assisted enzymatic hydrolysis[J].Food and Bioprocess Technology, 2019,12(6):910-918.
[6] 张瑞娟, 柯莉娜, 郑静, 等.鲍鱼内脏多糖的提取、纯化及其抗氧化和抑菌活性[J].厦门大学学报(自然科学版), 2018,57(1):58-64.
ZHANG R J, KE L N, ZHENG J, et al.Extraction,purification and antioxidant and antibacterial activity analysis of polysaccharides from abalone viscera[J].Journal of Xiamen University(Natural Science), 2018,57(1):58-64.
[7] GONG F, CHEN M F, CHEN J L, et al.Boiled abalone byproduct peptide exhibits anti-tumor activity in HT1080 cells and HUVECs by suppressing the metastasis and angiogenesis in vitro[J].Journal of Agricultural and Food Chemistry, 2019,67(32):8 855-8 867.
[8] LIU B X, JIA Z, LI C C, et al.Hypolipidemic and anti-atherogenic activities of crude polysaccharides from abalone viscera[J].Food Science & Nutrition, 2020,8(5):2 524-2 534.
[9] 任鼎鼎, 黄梦怡, 郑惠娜, 等.皱纹盘鲍(Haliotis discus hannai Ino)肌肉酶解产物制备及其免疫活性研究[J].食品与发酵工业, 2020,46(23):21-28.
REN D D, HUANG M Y, ZHENG H N, et al.Preparation and immunoactivity of enzymatically hydrolyzed products of abalone (Haliotis discus hannai Ino) muscle[J].Food and Fermentation Industries, 2020,46(23):21-28.
[10] 张雪妍, 秦小明, 高加龙, 等.牡蛎酶解工艺优化及其酶解产物对小鼠睾酮分泌的影响[J].广东海洋大学学报, 2019,39(3):96-102.
ZHANG X Y,QIN X M,GAO J L, et al.Optimization of enzymatic hydrolysis from crassostrea gigas and effects of its enzymatic hydrolysate on TM3 leydig cells[J].Journal of Guangdong Ocean University, 2019,39(3):96-102.
[11] 李敏,王德民,李峰,等.药食同源中药抗衰老研究进展[J].食品与药品,2019,21(5):414-418.
LI M, WANG D M, LI F, et al.Progress in anti-aging function of traditional Chinese medicine with characteristics of medicine and food[J].Food and Drug, 2019,21(5):414-418.
[12] 李鹏程. 海洋生物资源高值利用研究进展[J].海洋与湖沼, 2020,51(4):750-758.
LI P C.Research progress in high-value utilization of marine biological resources[J].Oceanologia et Limnologia Sinica, 2020,51(4):750-758.
[13] 王力, 肖嵋方, 刘斌, 等.海洋生物活性物质抗衰老作用研究进展[J].食品工业科技, 2021,42(22):433-441.
WANG L, XIAO M F, LIU B, et al.Research progress on the anti-aging effect of marine bioactive substances[J].Science and Technology of Food Industry, 2021,42(22):433-441.
[14] MUDD N, LICEAGA A M.Caenorhabditis elegans as an in vivo model for food bioactives:A review[J].Current Research in Food Science,2022,5:845-846.
[15] 迟东泽, 何源, 刘芳芳, 等.鹿鞭醇提物对秀丽隐杆线虫衰老的影响[J].食品工业科技, 2021,42(10):327-335.
CHI D Z, HE Y,LIU F F, et al.Effect of ethanol extracts of penis cervi on anti-aging in caenorhabditis elegans[J].Science and Technology of Food Industry, 2021,42(10):327-335.
[16] 王雪. 鹿茸醇提物对秀丽隐杆线虫抗衰老机制的研究[D].长春:吉林农业大学, 2020.
WANG X Anti-aging mechanism of velvet antler extract on Caenorhabditis elegans[D].Changchun:Jilin Agricultural University,2020.
[17] 王力, 肖嵋方, 陈弘培, 等.牡蛎多肽组分OE-I抗氧化活性及其对秀丽隐杆线虫抗衰老作用[J].食品科学, 2022,43(3):152-160.
WANG L, XIAO M F, CHEN H P, et al.Antioxidant activity of oyster peptide fraction OE-I and its anti-aging effect on caenorhabditis elegans[J].Food Science, 2022,43(3):152-160.
[18] YANG X Y, WANG H, LI T, et al.Nobiletin delays aging and enhances stress resistance of Caenorhabditis elegans[J].International Journal of Molecular Sciences, 2020,21(1):341.
[19] ZHANG M H, YANG X, XU W, et al.Evaluation of the effects of three sulfa sweeteners on the lifespan and intestinal fat deposition in C.elegans[J].Food Research International, 2019,122:66-76.
[20] 宋兵兵. 蓝莓和苹果皮提取物联合抗衰老活性及作用机制研究[D].广州:华南理工大学, 2020.
SONG B B.Antiaging activity of combination of blueberry and apple peel extracts:Mechanisms of action[D].Guangzhou:South China University of Technology, 2020.
[21] PARK S, KIM B, PARK S K.Supplementation with phosphatidylethanolamine confers anti-oxidant and anti-aging effects via hormesis and reduced insulin/IGF-1-like signaling in C.elegans[J].Mechanisms of Ageing and Development, 2021,197:111498.
[22] ZHANG Y, MI D Y, WANG J, et al.Constituent and effects of polysaccharides isolated from Sophora moorcroftiana seeds on lifespan, reproduction, stress resistance, and antimicrobial capacity in Caenorhabditis elegans[J].Chinese Journal of Natural Medicines, 2018,16(4):252-260.
[23] HUI H, XIN A Y, CUI H Y, et al.Anti-aging effects on Caenorhabditis elegans of a polysaccharide, O-acetyl glucomannan, from roots of Lilium davidii var.unicolor Cotton[J].International Journal of Biological Macromolecules, 2020,155:846-852.
[24] 熊磊. 枸杞多酚提取物抗衰老活性及其作用机制研究[D].广州:华南理工大学, 2021.
XIONG L. Study on anti-aging activity and mechanism of Lycium barbarum polyphenol extract.Guangzhou: South China University of Technology, 2021.
[25] BORA S,VARDHAN G S H,DEKA N,et al.Paraquat exposure over generation affects lifespan and reproduction through mitochondrial disruption in C.elegans[J].Toxicology,2021,447:152632.
[26] 林春秀, 林伊梓, 沈少丹, 等.苦瓜青钱柳复合茶对秀丽线虫的抗氧化作用[J].食品与发酵工业, 2020,46(23):178-183.
LIN C X, LIN Y Z, SHEN S D, et al. Antioxidant effect of a compound Momordica charantia and Cyclocarya paliurus tea on Caenorhabditis elegans. Food and Fermentation Industries, 2020, 46(23):178-183.
[27] LIN C X, CHEN Y, LIN Y Z, et al.Antistress and anti-aging activities of Caenorhabditis elegans were enhanced by Momordica saponin extract[J].European Journal of Nutrition, 2021,60(4):1 819-1 832.
Options
文章导航

/

〈 〉

技术支持：北京玛格泰克科技发展有限公司