致病菌盛行及抗生素耐药给人类健康造成巨大挑战,开发新型抗菌剂至关重要。该文开发一种简便的方法,用以合成具有广谱抗菌性的抗菌纳米制剂。以儿茶素作为还原剂和稳定剂,水热法制备儿茶素-ZnO/Ag纳米复合材料。通过紫外-可见分光光谱仪(ultraviolet-visible spectroscopy,UV-vis),透射电子显微镜(transmission electron microscope,TEM),能谱仪(energy dispersive spectroscopy,EDS),X射线光电子能谱仪(X-ray photoelectron spectroscopy,XPS)和傅里叶红外光谱仪(Fourier transform infrared spectroscopy,FTIR)对其形貌、结构进行表征,并测试其抗菌活性。UV-vis与TEM结果表明,主要是银纳米颗粒点缀在氧化锌纳米棒表面,组成了纳米复合材料。FTIR结果证实纳米复合材料与儿茶素之间存在强烈相互作用,表明成功合成亲水性儿茶素功能化-ZnO/Ag纳米复合材料。同时,抑菌实验结果表明,儿茶素-ZnO/Ag纳米复合材料对大肠杆菌、金黄色葡萄球菌和白色念珠菌有很强的抑制效果。水热法合成的儿茶素-ZnO/Ag纳米复合材料具有广谱抗菌性,有望成为解决细菌感染和抗生素耐药问题的新型抗菌剂。
It is crucial to develop new antimicrobial agents since the prevalence of pathogenic bacteria and antibiotic resistance poses great challenges to human health.To develop a simple method on synthesis of nanosized antibacterial agents with broad-spectrum antibacterial properties.Hydrothermal method was developed to synthesize the catechin functionalized ZnO/Ag nanocomposites wherein the catechin was regarded as the reducing agent and stabilizer.The morphology and structure of the prepared nanocomposites were characterized by ultraviolet-visible spectroscopy (UV-vis), transmission electron microscope (TEM), energy dispersive spectroscopy, X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy (FTIR).The antibacterial activity was also tested.The results of UV-vis and TEM showed that silver nanoparticles dot on the surface of ZnO nanorods to form nanocomposites.The FTIR measurement confirmed that there was a strong interaction between catechin and nanocomposites, indicating that the successful synthesis of hydrophilic catechin functionalized ZnO/Ag nanocomposites.The antibacterial test results showed that the catechin-ZnO/Ag nanocomposites exerted strong bacteriostatic effect on Escherichia coli, Staphylococcus aureus and Candida albicans.The catechin-ZnO/Ag nanocomposites synthesized by the hydrothermal method possess broad-spectrum antibacterial properties, and are expected to be a new antibacterial agent to solve the problem of bacterial infection and antibiotic resistance.
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