食品与发酵工业 >

2023 , Vol. 49 >Issue 12: 194 - 201

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.033135

研究报告

浒苔模拟肠胃液消化水解液中血管紧张素转化酶抑制肽的分离纯化与鉴定

  • 张迪 ,
  • 罗威 ,
  • 梁海娣 ,
  • 王宇晗 ,
  • 郭天赋
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  • 1(广东海洋大学 食品科技学院,广东 湛江,524088)
    2(湛江幼儿师范专科学校, 广东 湛江,524084)
    3(岭南师范学院 基础教育学院,广东 湛江, 524037)
    4(广东省水产品加工与安全重点实验室,广东 湛江,524088)
    5(广东省海洋食品工程技术研究中心,广东 湛江,524088)
    6(广东普通高等学校水产品深加工重点实验室,广东 湛江,524088)
博士,讲师(罗威副教授为通信作者,E-mail:luo781126@sina.com)

收稿日期: 2022-07-29

  修回日期: 2022-08-31

  网络出版日期: 2023-07-13

基金资助

广东省普通高校特色创新类项目(2021KTSCX322)

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Isolation and purification of angiotensin-converting enzyme inhibitory peptides from the digestive hydrolysate of simulated gastrointestinal fluid of the Enteromorpha clathrata

  • ZHANG Di ,
  • LUO Wei ,
  • LIANG Haidi ,
  • WANG Yuhan ,
  • GUO Tianfu
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  • 1(College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China)
    2(Zhanjiang Preschool Education College, Zhanjiang 524084, China)
    3(College of Basic Education, Lingnan Normal University, Zhanjiang 524037, China)
    4(Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Zhanjiang 524088, China)
    5(Marine Food Engineering and Technology Research Center of Guangdong Provence, Zhanjiang 524088, China)
    6(Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Zhanjiang 524088, China)

Received date: 2022-07-29

  Revised date: 2022-08-31

  Online published: 2023-07-13

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摘要

为从浒苔模拟胃肠道消化产物中制备血管紧张素转化酶(angiotensin-converting enzyme,ACE)抑制肽,并鉴定其结构,该研究以浒苔为原料,模拟肠胃液消化条件对其进行水解,以体外ACE抑制率为指标,并采用超滤、凝胶色谱、离子色谱和反向色谱对浒苔模拟肠胃消化水解液进行分离纯化和筛选,以期获得纯度较高的ACE抑制肽,同时采用高效液相-四级杆飞行时间串联质谱对ACE抑制肽的氨基酸序列进行分析鉴定。结果表明,浒苔模拟肠胃液消化水解液中的ACE抑制活性肽主要分布在<3 000 u的超滤组分中,经分离纯化筛选得到一种新型ACE抑制肽,其分子质量为348.1 Da,肽序列为Leu-Tyr,半数抑制浓度IC50值为(0.12±0.01) mg/mL,与模拟消化产物相比,ACE抑制肽的IC50值纯化了11.17倍。该研究利用浒苔模拟肠胃液消化水解液制备ACE抑制肽,可以为浒苔的高值化利用提供理论参考。

关键词： 浒苔; 模拟胃肠道消化; 血管紧张素转化酶抑制肽; 分离纯化

本文引用格式

张迪 , 罗威 , 梁海娣 , 王宇晗 , 郭天赋 . 浒苔模拟肠胃液消化水解液中血管紧张素转化酶抑制肽的分离纯化与鉴定[J]. 食品与发酵工业, 2023 , 49(12) : 194 -201 . DOI: 10.13995/j.cnki.11-1802/ts.033135

Abstract

In order to prepare angiotensin-converting enzyme (ACE) inhibitory peptides from simulated gastrointestinal digestion products of Enteromorpha clathrata and to characterize their structures, the E.clathrata was used as raw material for preparation of hydrolysis of simulated gastrointestinal digestion products.And in vitro ACE inhibition rate was used as an indicator.The amino acid sequences of the ACE inhibitory peptides were analyzed and identified by high performance liquid-quadrupole time-of-flight tandem mass spectrometry.The results showed that the ACE inhibitory active peptide in the hydrolysate of simulated gastrointestinal digestion of E.clathrata was mainly distributed in the ultrafiltration fraction with H4.A new ACE inhibitory peptide was found with molecular weight of 348.1 Da and peptide sequence of Leu-Tyr was identified after isolation and purification.And the IC50 value of ACE inhibitory peptide was (0.12±0.01) mg/mL and purified by 11.17-fold.Thus, E.clathrata is a kind of beneficial and nutritional food and its simulated gastrointestinal digestive product contains ACE inhibitory peptide, which is a pharmaceutical component for the treatment of hypertension.E.clathrata has potential as an ingredient for hypotensive health products.

Key words： Enteromorpha clathrata; simulation of gastrointestinal digestion; angiotensin-converting enzyme inhibitory peptide; purification and identification

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