食品与发酵工业 >

2023 , Vol. 49 >Issue 13: 152 - 158

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.031601

研究报告

食源抗氧化肽的制备与活性筛选

  • 张李伟 ,
  • 张铂瑾 ,
  • 姜岩 ,
  • 崔永泉 ,
  • 殷佳艺 ,
  • 张英华
展开
  • 1(国家食品药品监督管理总局食品审评中心,北京,100070)
    2(东北农业大学 食品学院,黑龙江 哈尔滨,150030)
第一作者:博士,副研究员(张英华教授为通信作者,E-mail:yinghuazhang@neau.edu.cn)

收稿日期: 2022-03-30

  修回日期: 2022-06-23

  网络出版日期: 2023-08-07

收起

Preparation and activity screening of food-derived antioxidant peptides

  • ZHANG Liwei ,
  • ZHANG Bojin ,
  • JIANG Yan ,
  • CUI Yongquan ,
  • YIN Jiayi ,
  • ZHANG Yinghua
Expand
  • 1(Center of Foods Evaluation, State Administration for Market Regulation, Beijing 100070, China)
    2(Food Science College, Northeast Agricultural University, Harbin 150030, China)

Received date: 2022-03-30

  Revised date: 2022-06-23

  Online published: 2023-08-07

Fold

摘要

过多的活性氧自由基会导致细胞凋亡甚至组织损伤,引发慢性疾病,采用食源性活性肽辅助疾病的预防和治疗具有一定的开发潜力。该研究选择α-乳白蛋白、酪蛋白、米糠蛋白、麦谷蛋白和火麻仁蛋白,进行碱性蛋白酶单酶水解、胃蛋白酶与胰液素双酶解两种方式制备抗氧化肽,并测定水解产物的抗氧化活性。结果表明,碱性蛋白酶水解条件下的样品活性更高,α-乳白蛋白、酪蛋白、米糠蛋白和麦谷蛋白水解样的抗氧化活性相近且显著高于火麻蛋白(P<0.05)。将4种抗氧化活性较高的蛋白的水解样品进行凝胶色谱分离纯化,测定活性后发现,分子质量越小的分离组分活性越高。利用液相色谱-质谱联用对抗氧化肽进行序列鉴定,获得6条三肽和5条四肽,其中WVC、LFY和YWL具有抗氧化活性潜力。

关键词： 食源蛋白; 抗氧化肽; 水解产物; 分离纯化; 液相色谱-质谱联用

本文引用格式

张李伟 , 张铂瑾 , 姜岩 , 崔永泉 , 殷佳艺 , 张英华 . 食源抗氧化肽的制备与活性筛选[J]. 食品与发酵工业, 2023 , 49(13) : 152 -158 . DOI: 10.13995/j.cnki.11-1802/ts.031601

Abstract

Apoptosis and tissue damage could be caused by excess reactive oxygen radicals, leading to chronic diseases. Food-derived antioxidant peptides show some potential in the prevention and treatment of diseases. α- lactalbumin, casein, rice bran protein, wheat gluten, and hemp kernel protein were selected to prepare antioxidant peptides, hydrolyzed by alkaline protease hydrolysis and a combination of pepsin and pancreatin respectively, and the antioxidant activity of the hydrolysates were measured. Results showed that the activity of hydrolysates was higher when hydrolyzed by alkaline protease. Moreover, the activity of the hydrolysates of α-lactalbumin, casein, rice bran protein, and glutenin was similar and significantly higher than that of hemp seed protein (P<0.05). Gel chromatography was used to isolate and purify the hydrolysates of four proteins with high antioxidant activity. Determination of the activity of different fractions revealed that the smaller the molecular weight, the higher the antioxidant activity. Finally, six tripeptides and five tetrapeptides were identified from α-lactalbumin, among which WVC, LFY, and YWL were antioxidant peptides with high antioxidant activity.

Key words： food-derived protein; antioxidant peptide; hydrolysate; separation and purification; liquid chromatography-mass spectrometry

参考文献

[1] WANG L, SONG X, CUI H, et al.Antifatigue effects of peptide isolated from sheep placenta[J].Chinese Herbal Medicines, 2018, 10(3):279-284.
[2] CHEN H Q, WANG S, ZHOU A M, et al.A novel antioxidant peptide purified from defatted round scad (Decapterus maruadsi) protein hydrolysate extends lifespan in Caenorhabditis elegans[J].Journal of Functional Foods, 2020, 68:103907.
[3] 曹月. 棉籽肽的制备及其对Ⅱ型糖尿病小鼠治疗作用的研究[D].长春:吉林大学, 2016.
CAO Y.Preparation cottonseed peptide and its therapeutic effect in mice of type Ⅱ diabetes[D].Changchun:Jilin University, 2016.
[4] AHN C B, JE J Y, CHO Y S.Antioxidant and anti-inflammatory peptide fraction from salmon byproduct protein hydrolysates by peptic hydrolysis[J].Food Research International, 2012, 49(1):92-98.
[5] 张艳. 两种海洋抗氧化肽预防UVB致小鼠皮肤癌的效应及可能机理研究[D].青岛:青岛大学, 2016.
ZHANG Y.The preventive effects of two marine anti-oxidant peptides against UVB induced mouse skin cancer and exploration of potential mechanism[D].Qingdao:Qingdao University, 2016.
[6] ROZEK T, WEGENER K L, BOWIE J H, et al.The antibiotic and anticancer active aurein peptides from the Australian Bell Frogs Litoria aurea and Litoria raniformis the solution structure of aurein 1.2[J].European Journal of Biochemistry, 2000, 267(17):5330-5341.
[7] ZHANG Q, SU G W, ZHAO T T, et al.Neuroprotection of round scad (Decapterus maruadsi) hydrolysate in glutamate-damaged PC12 cells:Possible involved signaling pathways and potential bioactive peptides[J].Journal of Functional Foods, 2020, 64:103690.
[8] 张文敏, 张健, 周浩纯, 等.亚麻籽粕制备小分子抗氧化活性肽[J].食品科学, 2020,41(8):36-44.
ZHANG W M, ZHANG J, ZHOU H C et al.Preparation of small molecular antioxidant peptides from flaxseed meal[J].Food Science, 2020, 41(8):36-44.
[9] WU D, LI M Q, DING J, et al.Structure-activity relationship and pathway of antioxidant shrimp peptides in a PC12 cell model[J].Journal of Functional Foods, 2020, 70:103978.
[10] YANG J, HUANG J C, DONG X L, et al.Purification and identification of antioxidant peptides from duck plasma proteins[J].Food Chemistry, 2020, 319:126534.
[11] HABINSHUTI I, MU T H, ZHANG M.Ultrasound microwave-assisted enzymatic production and characterisation of antioxidant peptides from sweet potato protein[J].Ultrasonics Sonochemistry, 2020, 69:105262.
[12] ZENG W C, SUN Q, ZHANG W H, et al.Antioxidant activity in vivo and biological safety evaluation of a novel antioxidant peptide from bovine hair hydrolysates[J].Process Biochemistry, 2017, 56:193-198.
[13] 于洋, 祁艳霞, 靳艳.乳源生物活性肽研究进展[J].食品与发酵工业, 2017, 43(9):259-266.
YU Y, QI Y X, JIN Y.Milk-derived bioactive peptides:A review[J].Food and Fermentation Industries, 2017, 43(9):259-266.
[14] BALLATORE M B, DEL ROSARIO BETTIOL M, VANDEN BRABER N L, et al.Antioxidant and cytoprotective effect of peptides produced by hydrolysis of whey protein concentrate with trypsin[J].Food Chemistry, 2020, 319:126472.
[15] 陈季旺, 姚惠源.几种常见植物蛋白生物活性肽的研究概述[J].中国油脂, 2003, 28(1):37-40.
CHEN J W, YAO H Y.Research summarize of several familiar active peptides derived from vegetable protein[J].China Oils and Fats, 2003, 28(1):37-40.
[16] 张美莉, 侯文娟, 杨立风.植物蛋白源生物活性肽的研究进展[J].中国食物与营养, 2010, 16(11):33-36.
ZHANG M L, HOU W J, YANG L F.Research advancement of bioactive peptides derived from plant protein[J].Food and Nutrition in China, 2010, 16(11):33-36.
[17] JIANG X, CUI Z Q, WANG L H, et al.Production of bioactive peptides from corn gluten meal by solid-state fermentation with Bacillus subtilis MTCC5480 and evaluation of its antioxidant capacity in vivo[J].LWT, 2020, 131:109767.
[18] JI D W, UDENIGWE C C, AGYEI D.Antioxidant peptides encrypted in flaxseed proteome:An in silico assessment[J].Food Science and Human Wellness, 2019, 8(3):306-314.
[19] CHAI T T, XIAO J B, MOHANA DASS S, et al.Identification of antioxidant peptides derived from tropical jackfruit seed and investigation of the stability profiles[J].Food Chemistry, 2021, 340:127876.
[20] DING J, LIANG R, YANG Y Y, et al.Optimization of pea protein hydrolysate preparation and purification of antioxidant peptides based on an in silico analytical approach[J].LWT, 2020, 123:109126.
[21] GUO H Q, WANG Y X, HE Q X, et al.In silico rational design and virtual screening of antioxidant tripeptides based on 3D-QSAR modeling[J].Journal of Molecular Structure, 2019, 1193:223-230.
[22] ZHENG L, ZHAO Y J, DONG H Z, et al.Structure-activity relationship of antioxidant dipeptides:Dominant role of Tyr, Trp, Cys and Met residues[J].Journal of Functional Foods, 2016, 21:485-496.
[23] DENG B C, LONG H R, TANG T Y, et al.Quantitative structure-activity relationship study of antioxidant tripeptides based on model population analysis[J].International Journal of Molecular Sciences, 2019, 20(4):995.
[24] IRSHAD I, KANEKANIAN A, PETERS A, et al.Antioxidant activity of bioactive peptides derived from bovine casein hydrolysate fractions[J].Journal of Food Science and Technology, 2015, 52(1):231-239.
[25] KONG X Z, ZHOU H M, HUA Y F.Preparation and antioxidant activity of wheat gluten hydrolysates (WGHs) using ultrafiltration membranes[J].Journal of the Science of Food and Agriculture, 2008, 88(5):920-926.
[26] SHAZLY A B, MU H B, LIU Z M, et al.Release of antioxidant peptides from buffalo and bovine caseins:Influence of proteases on antioxidant capacities[J].Food Chemistry, 2019, 274:261-267.
[27] ORSINI DELGADO M C, NARDO A, PAVLOVIC M, et al.Identification and characterization of antioxidant peptides obtained by gastrointestinal digestion of amaranth proteins[J].Food Chemistry, 2016, 197:1160-1167.
[28] AHMED A S, EL-BASSIONY T, ELMALT L M, et al.Identification of potent antioxidant bioactive peptides from goat milk proteins[J].Food Research International, 2015, 74:80-88.
[29] SAITO K, JIN D H, OGAWA T, et al.Antioxidative properties of tripeptide libraries prepared by the combinatorial chemistry[J].Journal of Agricultural and Food Chemistry, 2003, 51(12):3668-3674.
[30] CHEN N, CHEN J, YAO B, et al.QSAR study on antioxidant tripeptides and the antioxidant activity of the designed tripeptides in free radical systems[J].Molecules, 2018, 23(6):1407.
Options
文章导航

/

〈 〉

技术支持：北京玛格泰克科技发展有限公司