食品与发酵工业 >

2023 , Vol. 49 >Issue 13: 165 - 171

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.032983

研究报告

青钱柳多糖降血糖活性成分研究

  • 姚新伟 ,
  • 赵锦金 ,
  • 徐德平
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  • (江南大学 食品学院,江苏 无锡,214122)
第一作者:硕士研究生(徐德平副教授为通信作者,E-mail:xdp1219@sina.com)

收稿日期: 2022-07-13

  修回日期: 2022-08-19

  网络出版日期: 2023-08-07

基金资助

中央高校基本科研业务费专项资金资助项目(JUSRP21127)

收起

Hypoglycemic bioactivity of polysaccharide from Cyclocarya paliurus

  • YAO Xinwei ,
  • ZHAO Jinjin ,
  • XU Deping
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  • (School of Food Science and Technology, Jiangnan University, Wuxi 214122, China)

Received date: 2022-07-13

  Revised date: 2022-08-19

  Online published: 2023-08-07

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摘要

该研究旨在探究青钱柳多糖的结构及其降血糖活性。以青钱柳叶为原料,对水提醇沉得到的青钱柳粗多糖采用DEAE-纤维素柱、HW-55F凝胶柱及Sephacryl S400凝胶柱进行分离纯化,并利用动物实验筛选降血糖活性成分,通过核磁共振波谱分析鉴定化学结构。结果表明,青钱柳粗多糖及其DEAE-纤维素柱水洗脱样品B能够显著降低糖尿病小鼠的空腹血糖,提高口服葡萄糖耐受量。样品B再经进一步分离纯化得到具有降血糖活性的多糖CPP-1。根据其核磁共振波谱分析结果,初步推断该多糖由(1→2)-β-D-葡萄糖构成主链,由β-D-葡萄糖醛酸乙酯构成侧链,侧链连接在β-D-葡萄糖C3上,该结构在青钱柳多糖的研究中为首次报道。

关键词： 青钱柳; 多糖; 降血糖; 分离纯化; 鉴定

本文引用格式

姚新伟 , 赵锦金 , 徐德平 . 青钱柳多糖降血糖活性成分研究[J]. 食品与发酵工业, 2023 , 49(13) : 165 -171 . DOI: 10.13995/j.cnki.11-1802/ts.032983

Abstract

The purpose of this study is to explore the structure and hypoglycemic activity of Cyclocarya paliurus polysaccharide. Cyclocarya paliurus crude polysaccharides obtained by water extraction and alcohol precipitation from the leaves of Cyclocarya paliurus were separated and purified by DEAE-cellulose column, HW-55F gel column and Sephacryl S400 gel column. The hypoglycemic active components were screened by animal experiments, and the chemical structure was identified by nuclear magnetic resonance. Results showed that Cyclocarya paliurus crude polysaccharide and its DEAE-cellulose column water elution sample B could significantly reduce the fasting blood glucose (FBG) and increase the oral glucose tolerance in diabetic mice. B sample was further separated and purified to obtain polysaccharide CPP-1 with hypoglycemic activity. According to the results of its NMR spectrum analysis, it was preliminarily inferred that the polysaccharide consisted of (1→2)-β-D-glucose as the main chain, and β-D-glucose ethyl ester as the side chain, which was connected to β-D-glucose C3. This structure was first reported in the study of Cyclocarya paliurus polysaccharide.

Key words： Cyclocarya paliurus; polysaccharide; hypoglycemic; isolation and purification; identification

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