该研究开发了一种简单、高效的菠萝叶转化生物乙醇、酵母蛋白质和纤维材料方法。菠萝叶通过机械压榨产生叶汁和纤维材料,每吨新鲜菠萝叶可产生0.75 t叶汁和0.12 t干纤维。菠萝叶汁自身的营养成分完全可以提供酿酒酵母生长所需,含有的糖分主要为葡萄糖和果糖,采用酿酒酵母进行厌氧发酵生产生物乙醇及酵母蛋白质。通过发酵参数优化,在温度30 ℃,初始pH 4.0,接种量10%(体积分数)的发酵条件下,乙醇的产量最高达12.88 g/L,干酵母粉可达 3.83 g/L。研究结果将菠萝叶转化为具有利用价值的生物原料,可为我国菠萝种植废弃物的综合利用提供参考。
This study developed a simple and efficient method that converted pineapple leaves into bioethanol, yeast protein, and fiber materials. Pineapple leaves produced leaf juice and fiber materials through mechanical pressing. Each ton of fresh pineapple leaves could produce 0.75 t leaf juice and 0.12 t dry fiber. The nutrient components of the pineapple leaf juice could completely provide the requirements for the growth of Saccharomyces cerevisiae. The sugar contained in the pineapple leaf juice was mainly glucose and fructose. Saccharomyces cerevisiae was adopted for anaerobic fermentation to produce bioethanol and yeast protein. Through the optimization of fermentation parameters, under the conditions of temperature 30 ℃, initial pH 4.0, and inoculum size of 10% (volume fraction), the maximum yield of ethanol was up to 12.88 g/L and the dry yeast powder was up to 3.83 g/L. The result of this study can provide an experimental reference for the comprehensive utilization of pineapple leaves.
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