综述与专题评论

基于量子点可视技术快速检测鲜食农产品中食源性致病菌的应用研究进展

  • 谷鑫 ,
  • 胡金庆 ,
  • 胡钰梅 ,
  • 刘佳妮 ,
  • 欧杰 ,
  • 潘迎捷 ,
  • 赵勇 ,
  • 刘海泉
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  • 1(上海海洋大学 食品学院,上海,201306)
    2(上海海洋大学 海洋学院,上海,201306)
    3(长岛自然资源局,山东 烟台,265800)
    4(上海水产品加工及贮藏工程技术研究中心,上海,201306)
    5(农业农村部水产品贮藏保鲜质量安全风险评估实验室(上海),上海,201306)
    6(上海海洋大学 食品热加工工程技术研究中心,上海,201306)
第一作者:谷鑫(硕士研究生)和胡金庆(硕士,工程师)为共同第一作者(赵勇教授和刘海泉副教授为共同通信作者,E-mail:yzhao@shou.edu.cn;hqliu@shou.edu.cn)

收稿日期: 2022-08-10

  修回日期: 2022-09-16

  网络出版日期: 2023-08-07

基金资助

上海市科委上海市“科技创新行动计划”农业领域项目(22N31900600)

Research progress in the application of quantum dots-based visualization technology for rapid detection of foodborne pathogens in fresh agricultural produces

  • GU Xin ,
  • HU Jinqing ,
  • HU Yumei ,
  • LIU Jiani ,
  • OU Jie ,
  • PAN Yingjie ,
  • ZHAO Yong ,
  • LIU Haiquan
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  • 1(College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China)
    2(College of Marine Science, Shanghai Ocean University, Shanghai 201306, China)
    3(Changdao District Bureau of Natural Resources, Yantai 265800, China)
    4(Shanghai Engineering Research Center of Aquatic Product Processing & Preservation, Shanghai 201306, China)
    5(Laboratory of Quality & Safety Risk Assessment for Aquatic Product on Storage and Preservation (Shanghai), Ministry of Agriculture and Rural Affairs, Shanghai 201306, China)
    6(Engineering Research Center of Food Thermal-processing Technology, Shanghai Ocean University, Shanghai 201306, China)

Received date: 2022-08-10

  Revised date: 2022-09-16

  Online published: 2023-08-07

摘要

鲜食农产品营养损失少,食用方便,已成为健康饮食新食材和膳食结构的重要组成部分。但由于在运输、贮藏等环节中易受到环境条件影响导致食源性致病菌的污染,从而引发腐败变质和食品安全事件,因此,针对鲜食农产品食源性致病菌的污染开发出操作简单、可视化的快速检测技术至关重要。量子点是一类能够产生荧光的纳米级半导体颗粒,具有稳定性好、可视性强、灵敏度高、量子产率高等优点,近年来在食品检测中的应用呈数量级增长。文章在总结了量子点的理化性质、合成制备的基础上,综述了其在食源性致病菌检测中的应用研究进展,并对量子点在鲜食农产品快速检测的主要挑战和应用前景进行阐述,以期为基于量子点的快速检测在食品安全中的广泛应用提供借鉴,为食品安全以及人类健康保驾护航。

本文引用格式

谷鑫 , 胡金庆 , 胡钰梅 , 刘佳妮 , 欧杰 , 潘迎捷 , 赵勇 , 刘海泉 . 基于量子点可视技术快速检测鲜食农产品中食源性致病菌的应用研究进展[J]. 食品与发酵工业, 2023 , 49(13) : 320 -327 . DOI: 10.13995/j.cnki.11-1802/ts.033283

Abstract

Fresh agricultural products have become new choices for healthy diet and an important part of dietary structure due to their low nutritional loss and ease of consumption. However, they are susceptible to contamination by foodborne pathogens during transportation and storage, which can lead to spoilage and food safety incidents. Therefore, it is crucial to develop a simple and visualized rapid detection technique for foodborne pathogens contamination of fresh agricultural products. Quantum dots (QDs) are a class of nano-scale semiconductor particles capable of generating fluorescence, and their applications in food detection have grown enormously in recent years because of their good stability, strong visibility, high sensitivity, and high quantum yield. Based on the physical and chemical properties, synthesis and preparation of QDs, the paper reviews the research progress in the application of QDs in the detection of foodborne pathogens, and describes the main challenges and future prospects of QDs in the rapid detection of fresh agricultural products, in order to provide strategies for the wide application of QDs-based rapid detection in food safety, so as to protect food safety and human health.

参考文献

[1] 左映平, 孙国勇.生物散斑激光技术及在生鲜农产品品质控制中的应用[J].江苏农业科学, 2021, 49(5):45-50.
ZUO Y P, SUN G Y.Biological speckle laser technology and its application in quality control of fresh agricultural products[J].Jiangsu Agricultural Sciences, 2021, 49(5):45-50.
[2] LI L L, ZHANG M, ADHIKARI B, et al.Recent advances in pressure modification-based preservation technologies applied to fresh fruits and vegetables[J].Food Reviews International, 2017, 33(5):538-559.
[3] BUMGARNER N R, SCHEERENS J C, KLEINHENZ M D.Nutritional yield:A proposed index for fresh food improvement illustrated with leafy vegetable data[J].Plant Foods for Human Nutrition, 2012, 67(3):215-222.
[4] PERERA N, GAMAGE T V, WAKELING L, et al.Colour and texture of apples high pressure processed in pineapple juice[J].Innovative Food Science & Emerging Technologies, 2010, 11(1):39-46.
[5] HAN J W, ZUO M, ZHU W Y, et al.A comprehensive review of cold chain logistics for fresh agricultural products:Current status, challenges, and future trends[J].Trends in Food Science & Technology, 2021, 109:536-551.
[6] RAMIREZ-HERNANDEZ A, GALAGARZA O A, ÁLVAREZ RODRIGUEZ M V, et al.Food safety in Peru:A review of fresh produce production and challenges in the public health system[J].Comprehensive Reviews in Food Science and Food Safety, 2020, 19(6):3323-3342.
[7] World Health Organization.Food safety[R/OL].WHO, (2020)[2022-04-30].https://www.who.int/NEWS-ROOM/FACT-SHEETS/DETAIL/FOOD-SAFETY.
[8] LIU Y, GAO Y, WANG T, et al. Detection of 12 common food-borne bacterial pathogens by Taq Man Real-Time PCR using a single set of reaction conditions[J]. Frontiers in Microbiology, 2019, 10. DOI: 10.3389/fmicb.2019.00222.
[9] DONG J, LU X, GUO W, et al.Research progress on isothermal amplification technology in the detection of foodborne pathogens[J].Food and Fermentation Industries, 2021,(8):256-260.
[10] XIN L, ZHANG L W.Recent progress in nucleic acid-microfluidic chips used for detection of foodborne pathogens:A review[J].Food Science, China, 2020, 41(23):266-272.
[11] POLTRONIERI P, DE BLASI M D, D'URSO O F.Detection of Listeria monocytogenes through real-time PCR and biosensor methods[J].Plant Soil and Environment, 2009, 55(9):363-369.
[12] JADHAV S R, SHAH R M, KARPE A V, et al.Detection of foodborne pathogens using proteomics and metabolomics-based approaches[J].Frontiers in Microbiology, 2018, 9:3132.
[13] 李海月, 黄继红, 张新武, 等.ATP生物发光法快速检测食源性致病菌的研究[J].河南工业大学学报(自然科学版), 2016, 37(1):67-71;128.
LI H Y, HUANG J H, ZHANG X W, et al.Study on rapid detection of food-borne pathogen by ATP bioluminescence method[J].Journal of Henan University of Technology (Natural Science Edition), 2016, 37(1):67-71;128.
[14] HU J Q, HUANG R N, WANG Y, et al.Development of duplex PCR-ELISA for simultaneous detection of Salmonella spp.and Escherichia coli O157:H7 in food[J].Journal of Microbiological Methods, 2018, 154:127-133.
[15] 李萌立, 李忠海, 李节, 等.量子点荧光探针技术在食源性致病菌检测中的应用[J].食品与机械, 2013, 29(5):241-244.
LI M L, LI Z H, LI J, et al.Application of quantum dots on detection of food borne pathogen[J].Food & Machinery, 2013, 29(5):241-244.
[16] 杨倩雯, 刘晓风, 曾海娟, 等.基于纳米材料的电化学免疫传感器及其在蛋白质检测中的研究进展[J].食品科学, 2021, 42(21):347-354.
YANG Q W, LIU X F, ZENG H J, et al.Electrochemical immunosensors and their application in the detection of proteins:A literature review[J].Food Science, 2021, 42(21):347-354.
[17] ABDEL-SALAM M, OMRAN B, WHITEHEAD K, et al.Superior properties and biomedical applications of microorganism-derived fluorescent quantum dots[J].Molecules, 2020, 25(19):1-5.
[18] XUE J P, WANG X F, JEONG J H, et al.Fabrication, photoluminescence and applications of quantum dots embedded glass ceramics[J].Chemical Engineering Journal, 2020, 383:123082.
[19] 覃爱苗, 赵路路, 杜为林, 等.近红外量子点的发光机理研究[J].光谱学与光谱分析, 2016, 36(7):2059-2065.
QIN A M, ZHAO L L, DU W L, et al.Luminescence mechanism of near-infrared quantum dots[J].Spectroscopy and Spectral Analysis, 2016, 36(7):2059-2065.
[20] RESCH-GENGER U, GRABOLLE M, CAVALIERE-JARICOT S, et al.Quantum dots versus organic dyes as fluorescent labels[J].Nature Methods, 2008, 5(9):763-775.
[21] LIU B, JIANG B, ZHENG Z P, et al.Semiconductor quantum dots in tumor research[J].Journal of Luminescence, 2019, 209:61-68.
[22] JUZENAS P, CHEN W, SUN Y P, et al.Quantum dots and nanoparticles for photodynamic and radiation therapies of cancer[J].Advanced Drug Delivery Reviews, 2008, 60(15):1600-1614.
[23] 高杨晨, 王猛, 张晓梅, 等.量子点荧光纳米材料的化学合成及其在潜手印显现中的应用[J].合成材料老化与应用, 2013, 42(2):40-46.
GAO Y C, WANG M, ZHANG X M, et al.Chemical synthesis of quantum dots fluorescent nanomaterials and their applications in development of latent fingerprints[J].Synthetic Materials Aging and Application, 2013, 42(2):40-46.
[24] 杜晴晴, 施科如, 王倩, 等.荧光量子点的生物合成方法研究进展[J].微生物学通报, 2017, 44(2):449-457.
DU Q Q, SHI K R, WANG Q, et al.Advance of approaches for fluorescent quantum dots biosynthesis[J].Microbiology China, 2017, 44(2):449-457.
[25] 徐传粉. 有机相中CdTe和ZnTe量子点的无膦化合成与性质表征[D].上海:华东师范大学, 2013.
XU C F.Phosphate-free synthesis and characterization of CdTe and ZnTe quantum dots[D].Shanghai:East China Normal University, 2013.
[26] 周蓓莹, 王明辉, 江莞.水相制备CdSe量子点及其ZnS核壳结构量子点[J].中国材料进展, 2017, 36(5):389-394.
ZHOU B Y, WANG M H, JIANG W.Preparation of CdSe and CdSe/ZnS core-shell quantum dots in aqueous phase[J].Materials China, 2017, 36(5):389-394.
[27] 李丽娜, 陈雯, 王舸泓, 等.水相合成CdTe量子点测定海藻中微量碲[J].分析试验室, 2021, 40(12):1414-1418.
LI L N, CHEN W, WANG G H, et al.Determination of trace tellurium in seaweed by synthesis of CdTe quantum dots in aqueous phase[J].Chinese Journal of Analysis Laboratory, 2021, 40(12):1414-1418.
[28] AKBARI M, RAHIMI-NASRABADI M, POURMASUD S, et al.CdTe quantum dots prepared using herbal species and microorganisms and their anti-cancer, drug delivery and antibacterial applications:A review[J].Ceramics International, 2020, 46(8):9979-9989.
[29] KOMINKOVA M, MILOSAVLJEVIC V, VITEK P, et al.Comparative study on toxicity of extracellularly biosynthesized and laboratory synthesized CdTe quantum dots[J].Journal of Biotechnology, 2017, 241:193-200.
[30] 张亚楠, 杨玲玲, 涂家薇, 等.金黄色葡萄球菌活细胞合成ZnSe量子点[J].高等学校化学学报, 2018, 39(6):1158-1163.
ZHANG Y N, YANG L L, TU J W, et al.Live-cell synthesis of ZnSe quantum dots in Staphylococcus aureus[J].Chemical Journal of Chinese Universities, 2018, 39(6):1158-1163.
[31] BAO H F, LU Z S, CUI X Q, et al.Extracellular microbial synthesis of biocompatible CdTe quantum dots[J].Acta Biomaterialia, 2010, 6(9):3534-3541.
[32] LAMOUREUX L, ADAMS P, BANISADR A, et al.An optical biosensor for detection of pathogen biomarkers from Shiga toxin-producing Escherichia coli in ground beef samples[C]//SPIE BiOS.Proc SPIE 9310, Frontiers in Biological Detection:From Nanosensors to Systems VII, San Francisco, California, USA.2015, 9310:7-14.
[33] HAHN M A, TABB J S, KRAUSS T D.Detection of single bacterial pathogens with semiconductor quantum dots[J].Analytical Chemistry, 2005, 77(15):4861-4869.
[34] GAZOULI M, LIANDRIS E, ANDREADOU M, et al.Specific detection of unamplified mycobacterial DNA by use of fluorescent semiconductor quantum dots and magnetic beads[J].Journal of Clinical Microbiology, 2010, 48(8):2830-2835.
[35] ZHAO Y, YE M Q, CHAO Q G, et al.Simultaneous detection of multifood-borne pathogenic bacteria based on functionalized quantum dots coupled with immunomagnetic separation in food samples[J].Journal of Agricultural and Food Chemistry, 2009, 57(2):517-524.
[36] HUANG A H, QIU Z G, JIN M, et al.High-throughput detection of food-borne pathogenic bacteria using oligonucleotide microarray with quantum dots as fluorescent labels[J].International Journal of Food Microbiology, 2014, 185:27-32.
[37] DU H, WANG X M, YANG Q L, et al.Quantum dot:Lightning invisible foodborne pathogens[J].Trends in Food Science & Technology, 2021, 110:1-12.
[38] 乔敏. 基于纳米材料的生物传感器在食品安全检测中的应用研究[J].粘接, 2021, 47(9):72-74;164.
QIAO M.Research on the application of biosensors based on nanomaterials in food safety detection[J].Adhesion, 2021, 47(9):72-74;164.
[39] XU L Z, LU Z, CAO L L, et al.In-field detection of multiple pathogenic bacteria in food products using a portable fluorescent biosensing system[J].Food Control, 2017, 75:21-28.
[40] 周环宇, 乔楠, 于大禹.碳量子点荧光探针在致病菌检测中的应用[J].化工新型材料, 2022, 50(5):21-26;31.
ZHOU H Y, QIAO N, YU D Y.Application of CQDs fluorescent probe in detection of pathogenic microorganism[J].New Chemical Materials, 2022, 50(5):21-26;31.
[41] CARVALHO F, GEORGE J, SHEIKH H M A, et al.Advances in screening, detection and enumeration of Escherichia coli using nanotechnology-based methods:A review[J].Journal of Biomedical Nanotechnology, 2018, 14(5):829-846.
[42] SAFARDOUST-HOJAGHAN H, SALAVATI-NIASARI M, AMIRI O, et al.Preparation of highly luminescent nitrogen doped graphene quantum dots and their application as a probe for detection of Staphylococcus aureus and E.coli[J].Journal of Molecular Liquids, 2017, 241:1114-1119.
[43] CRESPO R F, PEREZ O J P, RAMIREZ C.Total count of Salmonella typhimurium coupled on water soluble CdSe quantum dots by fluorescence detection[J].Journal of Electronic Materials, 2018, 47(8):4379-4384.
[44] CARRILLO-CARRIÓN C, SIMONET B M, VALCÁRCEL M.Colistin-functionalised CdSe/ZnS quantum dots as fluorescent probe for the rapid detection of Escherichia coli[J].Biosensors and Bioelectronics, 2011, 26(11):4368-4374.
[45] 徐焕焕, 余锦露, 孙雅静, 等.基于CdTe:Zn/ZnS量子点快速检测变形杆菌荧光免疫分析法的建立[J].分析试验室, 2019, 38(9):1065-1069.
XU H H, YU J L, SUN Y J, et al.Establishment of fluorescence immunoassay method for rapid detection of proteus based on CdTe:Zn/ZnS quantum dots[J].Chinese Journal of Analysis Laboratory, 2019, 38(9):1065-1069.
[46] RENUKA R M, ACHUTH J, CHANDAN H R, et al.A fluorescent dual aptasensor for the rapid and sensitive onsite detection of E.coli O157∶H7 and its validation in various food matrices[J].New Journal of Chemistry, 2018, 42(13):10807-10817.
[47] LIU Y S, ZHAO C, FU K Y, et al.Selective turn-on fluorescence detection of Vibrio parahaemolyticus in food based on charge-transfer between CdSe/ZnS quantum dots and gold nanoparticles[J].Food Control, 2017, 80:380-387.
[48] CHEN R, HUANG X L, LI J, et al.A novel fluorescence immunoassay for the sensitive detection of Escherichia coli O157:H7 in milk based on catalase-mediated fluorescence quenching of CdTe quantum dots[J].Analytica Chimica Acta, 2016, 947:50-57.
[49] WANG B B, WANG Q, CAI Z X, et al.Simultaneous, rapid and sensitive detection of three food-borne pathogenic bacteria using multicolor quantum dot probes based onmultiplex fluoroimmunoassay in food samples[J].LWT-Food Science and Technology, 2015, 61(2):368-376.
[50] PATEL M K, SINGH J, SINGH M K, et al.Tin oxide quantum dot based DNA sensor for pathogen detection[J].Journal of Nanoscience and Nanotechnology, 2013, 13(3):1671-1678.
[51] LIU Z P, SU X G.A novel fluorescent DNA sensor for ultrasensitive detection of Helicobacter pylori[J].Biosensors and Bioelectronics, 2017, 87:66-72.
[52] WANG B B, HUANG X, MA M H, et al.A simple quantum dot-based fluoroimmunoassay method for selective capturing and rapid detection of Salmonella Enteritidis on eggs[J].Food Control, 2014, 35(1):26-32.
[53] HU Y H, WANG C C, BING B, et al.Detection of Staphylococcus aureus using quantum clots as fluorescence labels[J].International Journal of Agricultural and Biological Engineering, 2014, 7(1):77-83.
[54] ANGENENDT P.Progress in protein and antibody microarray technology[J].Drug Discovery Today, 2005, 10(7):503-511.
[55] WINGREN C, BORREBAECK C A.Antibody-based microarrays[J].Methods in Molecular Biology (Clifton, N.J.), 2009, 509:57-84.
[56] SANVICENS N, PASCUAL N, TERESA FERNANDEZ-ARGUEELLES M, et al. Quantum dot-based array for sensitive detection of Escherichia coli[J]. Analytical and Bioanalytical Chemistry, 2011, 399(8): 2755-2762.
[57] WANG Y, SCHILL K M, FRY H C, et al.A quantum dot nanobiosensor for rapid detection of botulinum neurotoxin serotype E[J].ACS Sensors, 2020, 5(7):2118-2127.
[58] ZHOU J M, YANG Q B, LIANG C, et al.Detection of ochratoxin A by quantum dots-based fluorescent immunochromatographic assay[J].Analytical and Bioanalytical Chemistry, 2021, 413(1):183-192.
[59] LI Y F, XU L, FU X C, et al.A competitive immunoassay based on engineered magnetic/fluorescent nanoparticles and biolayer interferometry-based assay for T-2 toxin determination[J].Microchimica Acta, 2020, 187(9):514.
[60] WANG C Q, ZHANG W H, QIAN J, et al.A FRET aptasensor for sensitive detection of aflatoxin B1 based on a novel donor-acceptor pair between ZnS quantum dots and Ag nanocubes[J].Analytical Methods:Advancing Methods and Applications, 2021, 13(4):462-468.
[61] FOUBERT A, BELOGLAZOVA N V, GORDIENKO A, et al.Development of a rainbow lateral flow immunoassay for the simultaneous detection of four mycotoxins[J].Journal of Agricultural and Food Chemistry, 2017, 65(33):7121-7130.
[62] RESHMA V G, SYAMA S, SRUTHI S, et al.Engineered nanoparticles with antimicrobial property[J].Current Drug Metabolism, 2017, 18(11):1040-1054.
[63] KLOEPFER J A, MIELKE R E, NADEAU J L.Uptake of CdSe and CdSe/ZnS quantum dots into bacteria via purine-dependent mechanisms[J].Applied and Environmental Microbiology, 2005, 71(5):2548-2557.
[64] LUO Z H, WU Q S, ZHANG M, et al.Cooperative antimicrobial activity of CdTe quantum dots with rocephin and fluorescence monitoring for Escherichia coli[J].Journal of Colloid and Interface Science, 2011, 362(1):100-106.
[65] WANG L X, WU C S, FAN X D, et al.Detection of Escherichia coli O157:H7 and Salmonella in ground beef by a bead-free quantum dot-facilitated isolation method[J].International Journal of Food Microbiology, 2012, 156(1):83-87.
[66] CHEN X X, GAN M, XU H, et al.Development of a rapid and sensitive quantum dot-based immunochromatographic strip by double labeling PCR products for detection of Staphylococcus aureus in food[J].Food Control, 2014, 46:225-232.
[67] YAN Z Y, AI X X, SU Y L, et al.Intracellular biosynthesis of fluorescent CdSe quantum dots in Bacillus subtilis:A strategy to construct signaling bacterial probes for visually detecting interaction between Bacillus subtilis and Staphylococcus aureus[J].Microscopy and Microanalysis, 2016, 22(1):13-21.
[68] DUAN N, WU S J, YU Y, et al.A dual-color flow cytometry protocol for the simultaneous detection of Vibrio parahaemolyticus and Salmonella typhimurium using aptamer conjugated quantum dots as labels[J].Analytica Chimica Acta, 2013, 804:151-158.
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