食品与发酵工业 >

2023 , Vol. 49 >Issue 14: 80 - 87

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.033086

研究报告

母乳中β-胡萝卜素、维生素A及维生素A酯分布规律研究

  • 李韵唱 ,
  • 王龙琼 ,
  • 何小玲 ,
  • 丁德胜 ,
  • 王元楷 ,
  • 周凤兰 ,
  • 蔡甜 ,
  • 陈科伟
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  • 1(西南大学 食品科学学院,重庆,400715)
    2(中匈食品科学合作研究中心,重庆,400715)
    3(川渝共建特色食品重庆市重点实验室,重庆,400715)
    4(重庆医科大学附属第一医院妇产科,重庆,400016)
    5(西南大学 化学化工学院,重庆,400715)
硕士研究生(蔡甜讲师和陈科伟副教授为共同通信作者,E-mail:caitian@swu.edu.cn;chenkewei@swu.edu.cn)

收稿日期: 2022-07-24

  修回日期: 2022-09-02

  网络出版日期: 2023-08-30

基金资助

中国食品科学技术学会食品科技基金(2020-03);重庆市留创计划创新类项目(2022-66);西南大学创新研究2035先导计划重点项目(SWU-XDZD22007)

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Distribution of β-carotene, vitamin A, and vitamin A ester in breast milk

  • LI Yunchang ,
  • WANG Longqiong ,
  • HE Xiaoling ,
  • DING Desheng ,
  • WANG Yuankai ,
  • ZHOU Fenglan ,
  • CAI Tian ,
  • CHEN Kewei
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  • 1(College of Food Science, Southwest University, Chongqing 400715, China)
    2(Chinese-Hungarian Cooperative Research Centre for Food Science, Chongqing 400715, China)
    3(Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing, Chongqing 400715, China)
    4(Department of Obstetrics and Gynecology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China)
    5(School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China)

Received date: 2022-07-24

  Revised date: 2022-09-02

  Online published: 2023-08-30

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摘要

该研究利用超高效液相色谱/四极杆飞行时间质谱技术对不同泌乳阶段下的母乳中的β-胡萝卜素、维生素A及维生素A酯进行定性定量分析,通过差异性分析、主成分分析、相关性分析以及聚类热图分析研究三者在母乳中分布的内在规律。结果表明,母乳中的β-胡萝卜素和维生素A含量随着泌乳期的延长逐渐降低,二者含量具有相关性(皮尔逊相关系数为0.416)。母乳中的维生素A酯含量相对稳定,在3个泌乳阶段下共检测出9种维生素A酯,长链视黄醇酯为主要存在形式,二十四酸视黄醇酯含量最高。不同的维生素A酯间呈现一定的聚类性,辛酸视黄醇酯、二十二酸视黄醇酯与二十二碳烯酸视黄醇酯两两之间的代谢关联性最强(皮尔逊相关系数为0.91)。该研究结果可以为婴幼儿配方食品的研发提供借鉴与参考。

关键词： 母乳; β-胡萝卜素; 维生素A; 维生素A酯; 定性定量分析

本文引用格式

李韵唱 , 王龙琼 , 何小玲 , 丁德胜 , 王元楷 , 周凤兰 , 蔡甜 , 陈科伟 . 母乳中β-胡萝卜素、维生素A及维生素A酯分布规律研究[J]. 食品与发酵工业, 2023 , 49(14) : 80 -87 . DOI: 10.13995/j.cnki.11-1802/ts.033086

Abstract

Vitamin A and its precursors in breast milk play an important role in the growth and development of infants and young children. In this study, the qualitative and quantitative analyses of β-carotene, vitamin A, and vitamin A esters in breast milk under different lactation stages were performed with ultra-high performance liquid chromatography/quadrupole time of flight mass spectrometry. Their intrinsic patterns of distribution in breast milk were investigated by variance analysis, principal component analysis, correlation analysis, and cluster heat map analysis. Results showed that the contents of β-carotene and vitamin A in breast milk gradually decreased with the extension of the lactation period, and the two contents were correlated (Pearson correlation coefficient was 0.416). The content of vitamin A esters in breast milk was relatively stable, and a total of nine vitamin A esters were detected under the three lactation stages, with long-chain retinyl esters being the main form present and retinyl behenate having the highest content. There was a certain clustering among different vitamin A esters, and the metabolic correlation between retinyl caprylate, retinyl docosanoate, and retinyl docosahexanoate was the strongest (Pearson correlation coefficient was 0.91). The results of this study can provide a reference for formula research and the development of infant formula food.

Key words： breast milk; β-carotene; vitamin A; vitamin A esters; qualitative and quantitative analysis

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