食品与发酵工业 >

2023 , Vol. 49 >Issue 14: 88 - 93

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.033408

研究报告

青钱柳粗多糖CP50制备工艺优化及对黄嘌呤氧化酶抑制作用研究

  • 苏虹霞 ,
  • 高静 ,
  • 姜敏 ,
  • 贾尧
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  • (陕西理工大学 化学与环境科学学院,陕西省催化基础与应用重点实验室,陕西 汉中,723000)
硕士研究生(姜敏教授为通信作者,E-mail:minjiangsnut@sina.com)

收稿日期: 2022-08-23

  修回日期: 2022-09-16

  网络出版日期: 2023-08-30

基金资助

陕西省科技厅自然科学基金项目(2022JM-133);陕西理工大学2022年研究生创新基金项目(SLGYCX2225)

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Optimization of preparation technology for crude polysaccharide CP50 from Cyclocarya paliurus and its inhibitory effects on xanthine oxidase

  • SU Hongxia ,
  • GAO Jing ,
  • JIANG Min ,
  • JIA Yao
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  • (School of Chemical and Environmental Sciences, Shaanxi University of Technology, Shaanxi Key Laboratory of Catalysis, Hanzhong 723000, China)

Received date: 2022-08-23

  Revised date: 2022-09-16

  Online published: 2023-08-30

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摘要

青钱柳是珍贵的药食同源型植物,青钱柳多糖CP50是具有较强生物活性的果胶类酸性多糖。黄嘌呤氧化酶(xanthine oxidase,XO)是一种嘌呤分解代谢酶,与高尿酸血症和痛风形成有关。该研究采用正交法优化了从青钱柳干叶中制备青钱柳多糖CP50的最佳工艺为提取温度100 ℃、料液比1∶40(g∶mL)、提取时间2 h,此条件下多糖得率为1.67%。以黄嘌呤氧化酶作为靶点,并结合分子模拟手段探究了青钱柳多糖CP50对XO的抑制机理。试验结果显示,青钱柳多糖CP50(0~1 mg/mL)对XO有一定抑制作用,且对XO的抑制活性呈浓度依赖性,IC50为0.825 mg/mL。分子对接结果进一步表明,青钱柳多糖作用于XO时,CP50分子插入XO的疏水腔,占据催化中心,导致XO活性受到抑制而达到降低尿酸的效果。该研究旨在为青钱柳CP50作为一种有效的膳食多糖应用于高尿酸血症患者的日常饮食提供方法借鉴和数据支持。

关键词： 药食同源植物; 青钱柳多糖; 黄嘌呤氧化酶; 抑制作用; 分子对接

本文引用格式

苏虹霞 , 高静 , 姜敏 , 贾尧 . 青钱柳粗多糖CP50制备工艺优化及对黄嘌呤氧化酶抑制作用研究[J]. 食品与发酵工业, 2023 , 49(14) : 88 -93 . DOI: 10.13995/j.cnki.11-1802/ts.033408

Abstract

Cyclocarya paliurus is a kind of precious homologous plant for medicine and food. Polysaccharide from C. paliurus CP50 is the pectinic acidic polysaccharide with strong biological activity. Xanthine oxidase (XO) is a purine catabolism enzyme associated with hyperuricemia and gout. In this study, according to the orthogonal experiments, the extraction condition parameters of the polysaccharide CP50 from the dried leaves of C. paliurus were optimized as follows: extraction temperature 100 ℃, solid-liquid ratio 1∶40(g∶mL), extraction time 2 h, and the polysaccharide yield was 1.67%. The inhibition mechanism of CP50 was explored by using XO as the target and combined with molecular simulation methods. The results showed that CP50(0-1 mg/mL) haD a certain inhibitory effect on XO, and its inhibitory activity was concentration-dependent, with IC50 of 0.825 mg/mL. The molecular docking results showed that, CP50 molecules were inserted into the hydrophobic cavity of XO and occupied the catalytic center, bringing the inhibition activity of XO and the effects of reducing uric acid. The purpose of this study is to provide method reference and data support for the application of CP50 as a useful dietary polysaccharide in daily diet of patients with hyperuricemia.

Key words： medicinal and edible plants; Cyclocarya paliurus polysaccharide; xanthine oxidase; inhibition action; molecular docking

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