食品与发酵工业 >

2023 , Vol. 49 >Issue 14: 147 - 153

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.032027

研究报告

体外模拟消化对黑果腺肋花楸叶片抗氧化及抑制糖消化酶活性影响

  • 石志娇 ,
  • 韦桂芳 ,
  • 何旭华 ,
  • 徐涵 ,
  • 彭小伟 ,
  • 阚欢 ,
  • 刘云
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  • 1(西南林业大学 生命科学学院,云南 昆明,650224)
    2(云南省高校大健康类森林资源开发利用工程研究中心,云南 昆明,650224)
硕士研究生(阚欢教授和刘云高级实验师为共同通信作者,E-mail:13700650213@163.com;liuyun0402001@163.com)

收稿日期: 2022-04-19

  修回日期: 2022-06-10

  网络出版日期: 2023-08-30

基金资助

云南省基础研究计划项目(202201AT070050)

收起

Effects of simulated digestion in vitro on activities of antioxidant and sugar digestion enzyme inhibition in Aronia melanocarpa leaves

  • SHI Zhijiao ,
  • WEI Guifang ,
  • HE Xuhua ,
  • XU Han ,
  • PENG Xiaowei ,
  • KAN Huan ,
  • LIU Yun
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  • 1(College of Life Sciences, Southwest Forestry University, Kunming 650224, China)
    2(Forest Resources Exploitation and Utilization Engineering Research Center for Grand Health of Yunnan Provincial Universities, Kunming 650224, China)

Received date: 2022-04-19

  Revised date: 2022-06-10

  Online published: 2023-08-30

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摘要

该文通过对黑果腺肋花楸叶片进行体外模拟消化,对不同阶段叶片中多酚、黄酮的释放情况及抗氧化、抑制糖消化酶活性进行研究,并对其进行相关性分析。结果表明,叶片中多酚、黄酮在不同消化阶段的释放量均为口腔消化液>胃消化液>小肠消化液>大肠消化液。与未消化液相比,经体外模拟消化后,各消化液对DPPH自由基和ABTS阳离子自由基清除率,铁离子还原能力,α-葡萄糖苷酶和α-淀粉酶抑制率均呈下降趋势,而对羟自由基清除率在小肠部位呈极显著增长(P<0.001)。相关性分析中,除羟自由基清除率外,其他各活性指标与多酚、黄酮的释放量均呈正相关。综上,在体外模拟消化过程中,黑果腺肋花楸叶片中的活性成分多酚和黄酮逐渐被释放出来,并且表现出了较高的生物活性。

关键词： 黑果腺肋花楸; 体外模拟消化; 抗氧化; α-葡萄糖苷酶; α-淀粉酶

本文引用格式

石志娇 , 韦桂芳 , 何旭华 , 徐涵 , 彭小伟 , 阚欢 , 刘云 . 体外模拟消化对黑果腺肋花楸叶片抗氧化及抑制糖消化酶活性影响[J]. 食品与发酵工业, 2023 , 49(14) : 147 -153 . DOI: 10.13995/j.cnki.11-1802/ts.032027

Abstract

The release amounts of polyphenols and flavonoids from Aronia melanocarpa leaves and their activities of antioxidant and sugar digestion enzyme inhibition were studied by simulated digestion in vitro, in which correlation was analyzed. Results showed that the release amounts of polyphenols and flavonoids in leaves at different digestive stages were as follows: oral digestive fluid > gastric digestive fluid > small intestinal digestive fluid > large intestine digestive fluid. Compared with undigested fluid, DPPH radical and ABTS cationic radical scavenging rate, ferric reducing power, α-glucosidase, and α-amylase inhibition rate of each digestive fluid were all decreased after simulated digestion in vitro, while hydroxyl radical scavenging rate increased significantly in the small intestine (P<0.001). Furthermore, the activity indexes except hydroxyl radical scavenging rate were positively correlated with the release of polyphenols and flavonoids in the correlation analysis. In conclusion, polyphenols and flavonoids were gradually released from the leaves of A. melanocarp during simulated digestion in vitro and showed high biological activity.

Key words： Aronia melanocarpa; simulated digestion in vitro; antioxidant; α-glucosidase; α-amylase

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