研究报告

甜叶菊提取物对高糖脂饮食鼠血脂及其肠道菌群的影响

  • 陈敏 ,
  • 尹明雨 ,
  • 赵一梦 ,
  • 王锡昌
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  • 1(上海海洋大学 食品学院,上海,201306)
    2(复旦大学 人类表型组研究院,上海,200438)
硕士研究生(赵一梦助理研究员和尹明雨为通信作者,E-mail:ymzhao@fudan.edu.cn;yin214300841@163.com)

收稿日期: 2022-08-04

  修回日期: 2022-09-07

  网络出版日期: 2023-08-30

基金资助

海丘食品营养与品质调控项目(No.D-8006-11-0171)

Effects of stevia extract on blood lipids and intestinal flora in mice fed with high sugar and fat diet

  • CHEN Min ,
  • YIN Mingyu ,
  • ZHAO Yimeng ,
  • WANG Xichang
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  • 1(College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China)
    2(Human Phenome Institute, Fudan University, Shanghai 200438, China)

Received date: 2022-08-04

  Revised date: 2022-09-07

  Online published: 2023-08-30

摘要

探究甜叶菊提取物对脂质代谢、氧化应激和肠道微生态的影响。选取健康昆明鼠,构建脂代谢紊乱模型,分析其血脂、氧化应激及其肠道菌群变化。甜叶菊提取物显著降低了血脂(甘油三酯、胆固醇、低密度脂蛋白和游离脂肪酸)含量,显著提高了血清中抗氧化酶[超氧化物歧化酶(superoxide dismutase, SOD)和谷胱甘肽氧化物酶(glutathione peroxidase, GSH-Px)]水平,降低了丙二醛含量。病理组织切片显示,甜叶菊提取物显著改善了肝脏和结肠组织的损伤。高通量测序结果显示提取物提高了肠道拟杆菌水平,降低了厚壁菌水平。甜叶菊提取物能够调节脂质代谢,维持氧化应激平衡,抑制肝脏脂质堆积,同时具有益生元活性。

本文引用格式

陈敏 , 尹明雨 , 赵一梦 , 王锡昌 . 甜叶菊提取物对高糖脂饮食鼠血脂及其肠道菌群的影响[J]. 食品与发酵工业, 2023 , 49(14) : 242 -249 . DOI: 10.13995/j.cnki.11-1802/ts.033208

Abstract

To investigate the effects of stevia extract on lipid metabolism, oxidative stress and intestinal microecology. We chose healthy Kunming mice, built models of lipid metabolic disorders, and examined changes in blood lipids, oxidative stress, and intestinal flora. Stevia extract significantly reduced serum lipids indices (triglycerides, cholesterol, low-density lipoprotein, and free fatty acids), considerably increased serum levels of antioxidant enzymes (superoxide dismutase and glutathione peroxidase), and decreased aldehyde content. Histopathological observation showed that stevia extract significantly reduced liver and colon tissue damage, such as data from high-throughput sequencing showed the stevia extract lowered the number of Firmicutes and raised the number of Bacteroidetes in the intestine. Stevia extract has prebiotic effect and can control lipid metabolism from anomalies, oxidative stress from out-of-balance, and liver fatty deposition.

参考文献

[1] GARCÍA-FERNÁNDEZ G, KROTTER A, GARCÍA-PÉREZ Á, et al.Pilot randomized trial of cognitive-behavioral treatment plus contingency management for quitting smoking and weight gain prevention among smokers with overweight or obesity[J].Drug and Alcohol Dependence,2022,236:109477.
[2] MAHMOUD H M, SALLAM R M, AYAD HENIN C M, et al.Synbiotics intake improves disturbed metabolism in a rat model of high fat diet-induced obesity:A potential role of adipose tissue browning[J].Obesity Medicine,2022,32:100414.
[3] 王佳. “肠—肝—脑”轴介导的番茄红素改善糖脂代谢紊乱及认知障碍的作用机制研究[D].杨凌:西北农林科技大学,2019.
WANG J.Lycopene attenuates the impairment of cognitive function via improving glycolipid metabolism dysfunction in gut-liver-brain axis[D].Yangling:Northwest A&F University, 2019.
[4] 尚延侠.运动对肥胖儿童少年血管内皮功能与血清淀粉样蛋白A等炎症因子的影响[D].上海:上海体育学院, 2011.
SHANG Y X.The effect of exercise on endothelial function and inflammatory factors such as acute-phase serum amyloid a in obese children and adolescents[D].Shanghai:Shanghai University of Sport, 2011.
[5] 尹畅. 外源性胆汁酸缓解肉鸡热应激作用的研究[D].北京:中国农业科学院,2021.
YIN C.The effects of exogenous bile acid on alleviating heat stress in broiler chickens[D].Beijing:Chinese Academy of Agricultural Sciences, 2021.
[6] 魏萌,蒋红利,史珂慧,等.益生菌改善尿毒症大鼠肠道氧化应激反应保护肠道屏障功能的研究[J].临床肾脏病杂志,2020,20(7):573-578.
WEI M, JIANG H L, SHI K H, et al.Probiotics improve intestinal oxidative stress to protect intestinal barrier function in uremic rats[J].Journal of Clinical Nephrology, 2020, 20(7):573-578.
[7] RAMIREZ L A, SNYDER E E, MATTSON D, et al.T cells contribute to lipid deposition in male and female dahl rats fed a high saturated fat diet[J].The FASEB Journal,2022.https://doi.org/10.1096/fasebj.2022.36.S1.0R832.
[8] WEI B, ZHANG B, DU A Q, et al.Saccharina japonica Fucan suppresses high fat diet-induced obesity and enriches fucoidan-degrading gut bacteria[J].Carbohydrate Polymers, 2022, 290:119411.
[9] KHOSRAVI A D, SEYED-MOHAMMADI S, TEIMOORI A, et al. The role of microbiota in colorectal cancer. Folia Microbiologica, 2022, 67(5):683-691.
[10] ZHA H, SI G N, WANG C Y, et al.Characteristics of the gut microbiota in Bifidobacterium catenulatum LI10 pretreated rats with lower levels of D-galactosamine-induced liver damage[J].Journal of Applied Microbiology,2022, 133(2):375-384.
[11] YANG S Q, XIONG Z H, XU T F, et al.Compound probiotics alleviate cadmium-induced intestinal dysfunction and microbiota disorders in broilers[J].Ecotoxicology and Environmental Safety, 2022, 234:113374.
[12] LI P, GAO M K, FU J H, et al.Dietary soya saponin improves the lipid metabolism and intestinal health of laying hens[J].Poultry Science,2022,101(4):101663.
[13] HE D, WU Q F, LU C Y, et al.Pyropia yezoensis porphyran alleviates metabolic disorders via modulating gut microbiota in high-sucrose-fed Drosophila melanogaster[J].Journal of the Science of Food and Agriculture,2022, 102(11):4 802-4 812.
[14] HE Y S, LUO R L, XIA M Y, et al.Orally administered diosgenin alleviates colitis in mice induced by dextran sulfate sodium through gut microbiota modulation and short-chain fatty acid generation[J].Journal of Medicinal Food,2022,25(3):261-271.
[15] 和贵文,和云凤,陈粉粉,等.麻风树叶提取物多酚与黄酮分析及对肉鸡盲肠微生物多样性的影响[J].江苏农业科学,2022,50(7):165-171.
HE G W, HE Y F, CHEN F F, et al.Analysis of polyphenols and flavonoids from Jatropha curcas leaf extract and their effects on cecal microbial diversity of broilers[J].Jiangsu Agricultural Science,2022,50(7):165-171.
[16] YU H, YANG G Q, SATO M, et al.Antioxidant activities of aqueous extract from Stevia rebaudiana stem waste to inhibit fish oil oxidation and identification of its phenolic compounds[J].Food Chemistry, 2017, 232, 379-386.
[17] 于慧,王锡昌,奚印慈.甜叶菊废弃物制得生物制剂的抗氧化活性研究[J].食品科学,2008, 29(8):65-69.
YU H, WANG X C, XI Y C.Study on antioxidant activity of biological preparation from Stevia rebaudiana waste[J].Food Science,2008, 29(8):65-69.
[18] 李丹,任晓静, 陈婷,等.维科天然生物制剂对干酪乳杆菌生长影响的初探[J].食品工业科技,2016,37(7):148-152;158.
LI D, REN X J, CHEN T, et al.Effect of WIC natural biologics on the growth of Lactobacillus casei[J].Science and Technology of Food Industry,2016,37(7):148-152;158.
[19] 任晓静,奚印慈,洪怡蓝,等.甜叶菊提取物抑菌作用的研究[J].食品工业科技,2014,35(14):174-177.
REN X J, XI Y C, HONG Y L, et al.Study on antibacterial effect of extraction from Stevia rebaudiana[J].Science and Technology of Food Industry, 2014,35(14):174-177.
[20] 张金山,刘艳,张立会,等.人参提取物及皂苷类成分对2型糖尿病作用机制的研究进展[J/OL].中药药理与临床, 2022.DOI:10.13412/j.cnki.zyyl.20220506.002.
ZHANG J S, LIU Y, ZHANG L H, et al.Research progress on the mechanism of action of ginseng extracts and saponins on type 2 diabetes mellitus[J/OL].Chinese Medicine Pharmacology and Clinical, 2022.DOI:10.13412/j.cnki.zyyl.20220506.002.
[21] 赵士博,吴忠港,郑淮远,等.松黄和野菊花及其联合应用对小鼠血糖及血脂的影响[J].中国老年学杂志,2022,42(8):1 950-1 952.
ZHAO S B, WU Z G, ZHENG H Y, et al.Effects of pine huang and wild chrysanthemum and their combined application on blood sugar and blood lipids in mice[J].Chinese Journal of Gerontology,2022,42(8):1 950-1 952.
[22] FAJRANI A M, SULCHAN M, MUIS S F, et al.Effect of black garlic on visceral fat, oxidative stress and insulin resistance in nonalcoholic fatty liver disease rats[J].Nutrition & Amp;Food Science,2021.DOI:10.1108/NFS-11-2020-0439.
[23] ASCHBACHER K, KORNFELD S, PICARD M, et al.Chronic stress increases vulnerability to diet-related abdominal fat, oxidative stress, and metabolic risk[J].Psychoneuroendocrinology,2014,46:14-22.
[24] 胡元利,谭晓梅,张文新.黄连总生物碱与盐酸小檗碱对小鼠溃疡性结肠炎治疗作用的比较[J].中药药理与临床,2011, 27(5):45-48.
HU Y L, TAN X M, ZHANG W X.Comparison of total alkaloids from Coptis Root and berberine for ulcerative colitis treatment[J].Pharmacology and Clinics of Chinese Materia Medica, 2011,27(5):45-48.
[25] LUISI M L E, LUCARINI L, BIFFI B, et al.Effect of Mediterranean diet enriched in high quality extra virgin olive oil on oxidative stress, inflammation and gut microbiota in obese and normal weight adult subjects[J].Frontiers in Pharmacology,2019,10:1366.
[26] ZONG L, LI H Y, LI S H.Effects of neoadjuvant chemotherapy combined with enteral nutrition on perioperative immunity, inflammation and intestinal flora in gastric cancer patients[J].Journal of the Balkan Union of Oncology,2019,24(3):1 113-1 119.
[27] COLLINS K H, PAUL H A, HART D A, et al.A high-fat high-sucrose diet rapidly alters muscle integrity, inflammation and gut microbiota in male rats[J].Scientific Reports,2016,6:37278.
[28] MELHEM, H, HANSMANNEL, F, BRESSENOT, A et al.Methyl-deficient diet promotes colitis and SIRT1-mediated endoplasmic reticulum stress[J].Gut, 2016, 65(4):595-606.
[29] VANDEPUTTE, D, FALONY, G, VIEIRA-SILVA, S, et al.Stool consistency is strongly associated with gut microbiota richness and composition, enterotypes and bacterial growth rates[J].Gut, 2016, 65(1):57-62.
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