食品与发酵工业 >

2023 , Vol. 49 >Issue 14: 280 - 287

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.033311

分析与检测

响应面-Box-Behnken设计优化地参中总黄酮含量检测方法以及不确定度评定

  • 江春阳 ,
  • 邓乔晟 ,
  • 王丹 ,
  • 许凌峰 ,
  • 杨青山 ,
  • 张华 ,
  • 杨勤 ,
  • 石汝杰 ,
  • 周浓
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  • 1(重庆三峡学院 生物与食品工程学院,重庆,404120)
    2(三峡库区道地药材绿色种植与深加工重庆市工程实验室,重庆,404120)
    3(重庆三峡医药高等专科学校 中医学院,重庆,404120)
硕士研究生(石汝杰副教授和周浓教授为共同通信作者,E-mail:yusrj@163.com;erhaizn@126.com)

收稿日期: 2022-08-12

  修回日期: 2022-09-30

  网络出版日期: 2023-08-30

基金资助

重庆市自然科学基金项目资助(cstc2019jcyj-msxmX0770);重庆市教委科学技术研究项目资助(KJQN202002712)

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Response surface-Box-Behnken design to optimize detection method and uncertainty evaluation of total flavonoids in Lycopus lucidus

  • JIANG Chunyang ,
  • DENG Qiaosheng ,
  • WANG Dan ,
  • XU Lingfeng ,
  • YANG Qingshan ,
  • ZHANG Hua ,
  • YANG Qin ,
  • SHI Rujie ,
  • ZHOU Nong
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  • 1(College of Biology and Food Engineering, Chongqing Three Gorges University, Chongqing 404120, China)
    2(The Chongqing Engineering Laboratory for Green Cultivation and Deep Processing of the Three Gorges Reservoir Area′s Medicinal Herbs, Chongqing 404120, China)
    3(College of Traditional Chinese Medicine, Chongqing Three Gorges Medical College, Chongqing 404120, China)

Received date: 2022-08-12

  Revised date: 2022-09-30

  Online published: 2023-08-30

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摘要

为优化地参总黄酮含量测定方法,该研究比较了NaNO2-Al(NO3)3-NaOH、AlCl3-KAc和三乙胺3种显色体系中6种对照品及供试品的吸收光谱曲线,对NaNO2-Al(NO3)3-NaOH体系显色剂加入量对总黄酮测量结果的影响进行了响应面- Box-Behnken设计优化,并对供试品溶液的制备,对照品溶液的制备,显色体系和标准曲线拟合进行了不确定度评估,计算合成不确定度并给出测量结果的扩展不确定度和置信水平。通过适应性分析,试验最终选择了NaNO2-Al(NO3)3-NaOH显色体系,最佳显色条件为50 g/L硝酸钠0.9 mL,100 g/L硝酸铝0.6 mL,1 moL/L氢氧化钠4.8 mL,显色温度42 ℃,以芦丁为标准品,在该条件下测得地参总黄酮含量为(91.052±2.75) mg。确定了地参总黄酮含量测定的最佳体系为NaNO2-Al(NO3)3-NaOH显色体系,及该体系下最佳测定条件,为地参总黄酮的测定提供了新的参考方法。

关键词： 地参; 总黄酮; 响应面; 不确定度; 显色体系

本文引用格式

江春阳 , 邓乔晟 , 王丹 , 许凌峰 , 杨青山 , 张华 , 杨勤 , 石汝杰 , 周浓 . 响应面-Box-Behnken设计优化地参中总黄酮含量检测方法以及不确定度评定[J]. 食品与发酵工业, 2023 , 49(14) : 280 -287 . DOI: 10.13995/j.cnki.11-1802/ts.033311

Abstract

To optimize the determination method for the total flavonoids in Lycopus lucidus, this study compared the absorption spectra of 6 reference substances and the samples in the three color reaction systems of NaNO2-Al(NO3)3-NaOH, AlCl3-Kac, and triethylamine. The response surface-Box-Behnken design optimization was carried out to optimize the influence of the color reagent dosage on the measurement results of total flavonoids in color reaction system of NaNO2-Al(NO3)3-NaOH. The uncertainty evaluation of the preparation of the test solution, the preparation of the reference solution, the color reaction system, and the standard curve fitting was carried out. The combined uncertainty also was calculated, and the expanded uncertainty and the confidence level of the measurement result were given. Through adaptability analysis, NaNO2-Al(NO3)3-NaOH color reaction system was finally selected in the experiment, and the optimal color reaction conditions were as follows: 0.9 mL of 50 g/L sodium nitrite, 0.6 mL of 100 g/L aluminum nitrate, 4.8 mL of 1 moL/L hydrogen sodium oxide, and color reaction temperature was 42 ℃. The content of total flavonoids was measured at (91.052±2.75) mg under optimal conditions, which provided a new reference method for the determination of total flavonoids.

Key words： Lycopus lucidus; total flavonoids; response surface; uncertainty; color reaction systems

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