食品与发酵工业 >

2023 , Vol. 49 >Issue 15: 187 - 192

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.032201

研究报告

对羟基苯甲酸-壳聚糖接枝物的合成及性能研究

  • 王皎龙
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  • (海南大学 食品科学与工程学院,海南 海口, 570228)
第一作者:硕士研究生(E-mail:2713366593@qq.com)

收稿日期: 2022-05-03

  修回日期: 2022-06-15

  网络出版日期: 2023-08-31

基金资助

海南省科技专项资助项目(ZDYF2022XDNY156);海南省高等学校科学研究项目(Hnky2022-5)

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Synthesis and properties of p-hydroxybenzoic acid-chitosan conjugate

  • WANG Jiaolong
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  • (College of Food Science and Engineering, Hainan University, Haikou 570228, China)

Received date: 2022-05-03

  Revised date: 2022-06-15

  Online published: 2023-08-31

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摘要

采用自由基接枝方法合成了不同接枝度的对羟基苯甲酸-壳聚糖(p-hydroxybenzoic acid-chitosan,PA-g-CS),以改善其应用性能。通过紫外-可见光谱、红外光谱、X射线衍射、热重分析和扫描电子显微镜对PA-g-CS进行表征,并考察其体外抗氧化和抑菌性能。结果表明,PA-g-CS分子中对羟基苯甲酸(p-hydroxybenzoic acid,PA)与壳聚糖(chitosan,CS)的羟基和氨基共价相连。与CS相比,PA-g-CS的结晶度和热稳定性降低,表面形态变为疏松多孔。体外抗氧化和抑菌实验表明,PA-g-CS的DPPH自由基清除活性,对革兰氏阴性(大肠杆菌)和阳性细菌(金黄色葡萄球菌)的抑制能力均显著增强。高接枝度的PA-g-CS抗氧化和抑菌活性更为优异。PA-g-CS有望应用于抗氧化剂和防腐剂等领域。

关键词： 壳聚糖; 对羟基苯甲酸; 结构表征; 生物活性

本文引用格式

王皎龙 . 对羟基苯甲酸-壳聚糖接枝物的合成及性能研究[J]. 食品与发酵工业, 2023 , 49(15) : 187 -192 . DOI: 10.13995/j.cnki.11-1802/ts.032201

Abstract

In this study, p-hydroxybenzoic acid-grafted chitosan (PA-g-CS) conjugates with different grafting degrees were synthesized by free radical-regulated grafting approach, so as to improve its application performance. The obtained PA-g-CS were characterized by UV-Vis spectra, FT-IR spectra, X-rays diffraction, thermogravimetric analysis and scanning electron microscopy. Results revealed that PA was successfully grafted onto the hydroxyl group and the amino group positions of CS. Compared with CS, PA-g-CS showed decreased crystallinity, thermal stability, as well as a loose and porous surface morphology. The antioxidant assays demonstrated that PA-g-CS exhibited remarkably enhanced DPPH-scavenging activities in vitro. Similarly, the synthesized PA-g-CS also significantly increased the antimicrobial activities toward Gram negative and positive bacteria, such as Escherichia coli and Staphylococcus aureus. Moreover, the products with a relatively higher grafting degree showed stronger biological activities. Our findings suggest that PA-g-CS conjugates have great potential application in the field of active packaging, antioxidants, and preservatives.

Key words： chitosan; p-hydroxybenzoic acid; characterization; biological activity

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