综述与专题评论

生物降解微塑料的机制和研究现状

  • 李清筱
展开
  • (河南工业贸易职业学院,河南 郑州,451191)
第一作者:硕士,讲师(通信作者,E-mail:lqx0445@sina.com)

收稿日期: 2023-02-14

  修回日期: 2023-04-14

  网络出版日期: 2023-08-31

基金资助

2023年度河南省重点研发与推广专项(科技攻关)项目(232102310301)

Mechanism and research status of biodegradable microplastics

  • LI Qingxiao
Expand
  • (Henan Industry and Trade Vocational College, Zhengzhou 451191, China)

Received date: 2023-02-14

  Revised date: 2023-04-14

  Online published: 2023-08-31

摘要

综述微塑料的种类、危害以及生物降解微塑料的影响因素以及昆虫、微生物、酶降解微塑料的现状及降解机制。根据研究现状,提出生物降解微塑料的研究方向:(1)研究昆虫肠道微生物菌群、宿主、营养摄食三方共协关系。利用代谢组学,研究脂肪、蛋白质以及以微塑料为碳源的代谢机制,研究昆虫分泌消化酶的种类和触发机制。(2)研究微生物分泌胞外、胞内降解酶的种类,借助人工合成技术,合成分泌酶,利用合成的外酶,加快对微塑料内部结构渗透、解聚。研究微生物降解微塑料的机制,以及影响降解的生物性和非生物性因素,确定微生物不竞争、和平共生的条件,采用多种微生物联合共协,配合微塑料物理化学降解方法,为微生物降解微塑料提供新思路。(3)找寻研究更多的降解酶。对目前确定能降解微塑料的酶类借助人工技术合成,研究昆虫摄食降解和人工合成酶、微生物降解和人工合成酶分别与微塑料物理化学降解方法联合使用的可行性。研究思路并行,学科交叉,降解方法共协,为生物降解微塑料提供理论基础。

本文引用格式

李清筱 . 生物降解微塑料的机制和研究现状[J]. 食品与发酵工业, 2023 , 49(15) : 311 -319 . DOI: 10.13995/j.cnki.11-1802/ts.035130

Abstract

The types and hazards of microplastics and the influencing factors of microplastic biodegradation are comprehensively described, as well as the current situation and the mechanism of microplastic degradation through insects, germs, and enzymes. The study direction of microplastic biodegradation is propose based on the current studies: (1) Study the trilateral correlated relations among insect enteric microbial communities, hosts and nutrient digestion. Make use of the metabonomics to study the metabolic mechanism of fat, protein, and microplastic carbon source, as well as the categories of the digestive enzymes produced by insects and their trigger mechanisms. (2) Study the categories of extracellular and intracellular degrading enzymes produced by germs, compose secretase with synthetic technology, and employ the synthesized extracellular enzyme to accelerate the permeation and degradation of the inside structure of the microplastic. Study the microplastic degradation mechanism and the biological and non-biological factors that impact the degradation, clarify the conditions for germs to live in harmony and free of competition, and provide new thoughts for microplastic biodegradation by applying the combination and coordination of kinds of germs in cooperation with physical and chemical microplastic degradation methods. (3) Search and study more degrading enzymes. Compose the enzymes which are known to be capable of degrading microplastics with artificial technologies, and combine the physical and chemical microplastic degradation methods respectively with the insect digestion degradation, the artificial synthetase and micro-biological degradation and the artificial synthetase degradation, and study their feasibility. Therefore, the theoretical foundation for microplastic degradation is laid through paralleled study thoughts, interdiscipline and coordinated degradation methods.

参考文献

[1] SKOCZINSKI P, KRAUSE L, RASCHKA A, et al.Current status and future development of plastics:Solutions for a circular economy and limitations of environmental degradation[J].Methods in Enzymology, 2021, 648:1-26.
[2] COLLARD F, GASPERI J, GABRIELSEN G W, et al.Plastic particle ingestion by wild freshwater fish:A critical review[J].Environmental Science & Technology, 2019, 53(22):12974-12988.
[3] LI J H, WANG Y T, WANG X D, et al.Crystalline characteristics, mechanical properties, thermal degradation kinetics and hydration behavior of biodegradable fibers melt-spun from polyoxymethylene/poly(l-lactic acid) blends[J].Polymers, 2019, 11(11):1753.
[4] CHEN Z, ZHAO W Q, XING R Z, et al.Enhanced in situ biodegradation of microplastics in sewage sludge using hyperthermophilic composting technology[J].Journal of Hazardous Materials, 2020, 384:121271.
[5] ABRUSCI C, PABLOS J L, CORRALES T, et al.Biodegradation of photo-degraded mulching films based on polyethylenes and stearates of calcium and iron as pro-oxidant additives[J].International Biodeterioration & Biodegradation, 2011, 65(3):451-459.
[6] AUTA H S, EMENIKE C U, JAYANTHI B, et al.Growth kinetics and biodeterioration of polypropylene microplastics by Bacillus sp.and Rhodococcus sp.isolated from mangrove sediment[J].Marine Pollution Bulletin, 2018, 127:15-21.
[7] JIANG S, SU T T, ZHAO J J, et al.Biodegradation of polystyrene by Tenebrio molitor, Galleria mellonella, and Zophobas atratus larvae and comparison of their degradation effects[J].Polymers, 2021, 13(20):3539.
[8] PENG B Y, SUN Y, WU Z Y, et al.Biodegradation of polystyrene and low-density polyethylene by Zophobas atratus larvae:Fragmentation into microplastics, gut microbiota shift, and microbial functional enzymes[J].Journal of Cleaner Production, 2022, 367:132987.
[9] PENG B Y, SU Y M, CHEN Z B, et al.Biodegradation of polystyrene by dark (Tenebrio obscurus) and yellow (Tenebrio Molitor) mealworms (Coleoptera:Tenebrionidae)[J].Environmental Science & Technology, 2019, 53(9):5256-5265.
[10] KUNDUNGAL H, GANGARAPU M, SARANGAPANI S, et al.Efficient biodegradation of polyethylene (HDPE) waste by the plastic-eating lesser waxworm (Achroia grisella)[J].Environmental Science and Pollution Research, 2019, 26(18):18509-18519.
[11] SURESH KESTI S, CHANDRABANDA THIMMAPPA S.First report on biodegradation of low density polyethyleneby rice moth larvae, Corcyra cephalonica(stainton)[J].The Holistic Approach to Environment, 2019, 9(4):79-83.
[12] LOU Y, EKATERINA P, YANG S S, et al.Biodegradation of polyethylene and polystyrene by greater wax moth larvae (Galleria mellonella L.) and the effect of co-diet supplementation on the core gut microbiome[J].Environmental Science & Technology, 2020, 54(5):2821-2831.
[13] YANG J, YANG Y, WU W M, et al.Evidence of polyethylene biodegradation by bacterial strains from the guts of plastic-eating waxworms[J].Environmental Science & Technology, 2014, 48(23):13776-13784.
[14] CUCINI C, LEO C, VITALE M, et al.Bacterial and fungal diversity in the gut of polystyrene-fed Alphitobius diaperinus (Insecta:Coleoptera)[J].Animal Gene, 2020, 17-18:200109.
[15] PEYDAEI A, BAGHERI H, GUREVICH L, et al.Mastication of polyolefins alters the microbial composition in Galleria mellonella[J].Environmental Pollution, 2021, 280:116877.
[16] WANG Z, XIN X, SHI X F, et al.A polystyrene-degrading Acinetobacter bacterium isolated from the larvae of Tribolium castaneum[J].Science of the Total Environment, 2020, 726:138564.
[17] KUNDUNGAL H, SYNSHIANG K, DEVIPRIYA S P.Biodegradation of polystyrene wastes by a newly reported honey bee pest Uloma sp.larvae:An insight to the ability of polystyrene-fed larvae to complete its life cycle[J].Environmental Challenges, 2021, 4:100083.
[18] ZHONG Z, NONG W Y, XIE Y C, et al.Long-term effect of plastic feeding on growth and transcriptomic response of mealworms (Tenebrio molitor L.)[J].Chemosphere, 2022, 287:132063.
[19] WANG S, SHI W, HUANG Z C, et al.Complete digestion/biodegradation of polystyrene microplastics by greater wax moth (Galleria mellonella) larvae:Direct in vivo evidence, gut microbiota independence, and potential metabolic pathways[J].Journal of Hazardous Materials, 2022, 423:127213.
[20] WANG S L, LYDON K A, WHITE E M, et al.Biodegradation of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) plastic under anaerobic sludge and aerobic seawater conditions:Gas evolution and microbial diversity[J].Environmental Science & Technology, 2018, 52(10):5700-5709.
[21] PRZEMIENIECKI S W, KOSEWSKA A, CIESIELSKI S, et al.Changes in the gut microbiome and enzymatic profile of Tenebrio molitor larvae biodegrading cellulose, polyethylene and polystyrene waste[J].Environmental Pollution, 2020, 256:113265.
[22] LUO L P, WANG Y M, GUO H Q, et al.Biodegradation of foam plastics by Zophobas atratus larvae (Coleoptera:Tenebrionidae) associated with changes of gut digestive enzymes activities and microbiome[J].Chemosphere, 2021, 282:131006.
[23] REN L, MEN L N, ZHANG Z W, et al.Biodegradation of polyethylene by Enterobacter sp.D1 from the guts of wax moth Galleria mellonella[J].International Journal of Environmental Research and Public Health, 2019, 16(11):1941.
[24] ZHANG J Q, GAO D L, LI Q H, et al.Biodegradation of polyethylene microplastic particles by the fungus Aspergillus flavus from the guts of wax moth Galleria mellonella[J].Science of the Total Environment, 2020, 704:135931.
[25] MONTAZER Z, HABIBI NAJAFI M B, LEVIN D B.In vitro degradation of low-density polyethylene by new bacteria from larvae of the greater wax moth, Galleria mellonella[J].Canadian Journal of Microbiology, 2021, 67(3):249-258.
[26] PEYDAEI A, BAGHERI H, GUREVICH L, et al.Impact of polyethylene on salivary glands proteome in Galleria melonella[J].Comparative Biochemistry and Physiology Part D:Genomics and Proteomics, 2020, 34:100678.
[27] YANG Y, YANG J, WU W M, et al.Biodegradation and mineralization of polystyrene by plastic-eating mealworms:Part 2.role of gut microorganisms[J].Environmental Science & Technology, 2015, 49(20):12087-12093.
[28] YIN C F, XU Y, ZHOU N Y.Biodegradation of polyethylene mulching films by a co-culture of Acinetobacter sp.strain NyZ450 and Bacillus sp.strain NyZ451 isolated from Tenebrio molitor larvae[J].International Biodeterioration & Biodegradation, 2020, 155:105089.
[29] KHAN S, NADIR S, SHAH Z U, et al.Biodegradation of polyester polyurethane by Aspergillus tubingensis[J].Environmental Pollution, 2017, 225:469-480.
[30] PARK S Y, KIM C G.Biodegradation of micro-polyethylene particles by bacterial colonization of a mixed microbial consortium isolated from a landfill site[J].Chemosphere, 2019, 222:527-533.
[31] YUAN J H, MA J, SUN Y R, et al.Microbial degradation and other environmental aspects of microplastics/plastics[J].Science of the Total Environment, 2020, 715:136968.
[32] HABIB S, IRUTHAYAM A, ABD SHUKOR M Y, et al.Biodeterioration of untreated polypropylene microplastic particles by Antarctic bacteria[J].Polymers, 2020, 12(11):2616.
[33] HADAD D, GERESH S, SIVAN A.Biodegradation of polyethylene by the thermophilic bacterium Brevibacillus borstelensis[J].Journal of Applied Microbiology, 2005, 98(5):1093-1100.
[34] HARSHVARDHAN K, JHA B.Biodegradation of low-density polyethylene by marine bacteria from pelagic waters, Arabian Sea, India[J].Marine Pollution Bulletin, 2013, 77(1-2):100-106.
[35] NANDA S, SAHU S S.Biodegradability of polyethylene by Brevibacillus, Pseudomonas, and Rhodococcus spp[J].New York Science Journal, 2010,3:57-60.
[36] RAJANDAS H, PARIMANNAN S, SATHASIVAM K, et al.A novel FTIR-ATR spectroscopy based technique for the estimation of low-density polyethylene biodegradation[J].Polymer Testing, 2012, 31(8):1094-1099.
[37] SIVAN A, SZANTO M, PAVLOV V.Biofilm development of the polyethylene-degrading bacterium Rhodococcus ruber[J].Applied Microbiology and Biotechnology, 2006, 72(2):346-352.
[38] GIACOMUCCI L, RADDADI N, SOCCIO M, et al.Biodegradation of polyvinyl chloride plastic films by enriched anaerobic marine consortia[J].Marine Environmental Research, 2020, 158:104949.
[39] YANG S S, DING M Q, HE L, et al.Biodegradation of polypropylene by yellow mealworms (Tenebrio molitor) and superworms (Zophobas atratus) via gut-microbe-dependent depolymerization[J].The Science of the Total Environment, 2021, 756:144087.
[40] ORHAN Y, BÜYÜKGÜNGÖR H.Enhancement of biodegradability of disposable polyethylene in controlled biological soil[J].International Biodeterioration & Biodegradation, 2000, 45(1-2):49-55.
[41] ASMITA K, SHUBHAMSINGH T, TEJASHREE S.Isolation of plastic degrading micro-organisms from soil samples collected at various locations in Mumbai, India[J].Environmental Science & Technology,2015,4:77-85.
[42] NOWAK B, PAJĄK J, DROZD-BRATKOWICZ M, et al.Microorganisms participating in the biodegradation of modified polyethylene films in different soils under laboratory conditions[J].International Biodeterioration & Biodegradation, 2011, 65(6):757-767.
[43] YAMADA-ONODERA K, MUKUMOTO H, KATSUYAYA Y, et al.Degradation of polyethylene by a fungus, Penicillium simplicissimum YK[J].Polymer Degradation and Stability, 2001, 72(2):323-327.
[44] DIAZ J M, PLUMMER S, TOMAS C, et al.Production of extracellular superoxide and hydrogen peroxide by five marine species of harmful bloom-forming algae[J].Journal of Plankton Research, 2018, 40(6):667-677.
[45] ZAMPOLLI J, ORRO A, MANCONI A, et al.Transcriptomic analysis of Rhodococcus opacus R7 grown on polyethylene by RNA-seq[J].Scientific Reports, 2021, 11:21311.
[46] KIRSTEIN I V, WICHELS A, GULLANS E, et al.The Plastisphere-uncovering tightly attached plastic “specific” microorganisms[J].PLoS One, 2019, 14(4):e0215859.
[47] GILAN I, HADAR Y, SIVAN A.Colonization, biofilm formation and biodegradation of polyethylene by a strain of Rhodococcus ruber[J].Applied Microbiology and Biotechnology, 2004, 65(1):97-104.
[48] HOWARD G T.Biodegradation of polyurethane:A review[J].International Biodeterioration & Biodegradation, 2002, 49(4):245-252.
[49] CARNIEL A, VALONI É, NICOMEDES J, et al.Lipase from Candida antarctica (CALB) and cutinase from Humicola insolens act synergistically for PET hydrolysis to terephthalic acid[J].Process Biochemistry, 2017, 59:84-90.
[50] OELSCHLÄGEL M, GRÖNING J A D, TISCHLER D, et al.Styrene oxide isomerase of Rhodococcus opacus 1CP, a highly stable and considerably active enzyme[J].Applied and Environmental Microbiology, 2012, 78(12):4330-4337.
[51] SAYYED R, WANI S J, SHAIKH S S, et al.Thermophilic PHB depolymerase of Stenotrophomonas sp., an isolate from the plastic contaminated site is best purified on Octyl-Sepharose CL-4B[J].Journal of Fermentation and Bioengineering, 2019,85:375-380.
[52] ALI SHAH A, HASAN F, HAMEED A, et al.Isolation and characterization of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) degrading bacteria and purification of PHBV depolymerase from newly isolated Bacillus sp.AF3[J].International Biodeterioration & Biodegradation, 2007, 60(2):109-115.
[53] KAWAI F.Polylactic acid (PLA)-degrading microorganisms and PLA depolymerases[M]//ACS Symposium Series.Washington, DC:American Chemical Society, 2010:405-414.
[54] UCHIDA H, SHIGENO-AKUTSU Y, NOMURA N, et al.Cloning and sequence analysis of poly(tetramethylene succinate) depolymerase from Acidovorax delafieldii strain BS-3[J].Journal of Bioscience and Bioengineering, 2002, 93(2):245-247.
[55] ABDEL-MOTAAL F F, EL-SAYED M A, EL-ZAYAT S A, et al.Biodegradation of poly (ε-caprolactone) (PCL) film and foam plastic by Pseudozyma japonica sp.nov, a novel cutinolytic ustilaginomycetous yeast species[J].3 Biotech, 2014, 4(5):507-512.
[56] SALVADOR M, ABDULMUTALIB U, GONZALEZ J, et al.Microbial genes for a circular and sustainable bio-PET economy[J].Genes, 2019, 10(5):373.
[57] WRIGHT R J, BOSCH R, LANGILLE M G I, et al.A multi-OMIC characterisation of biodegradation and microbial community succession within the PET plastisphere[J].Microbiome, 2021, 9(1):1-22.
文章导航

/