食品与发酵工业 >

2023 , Vol. 49 >Issue 16: 131 - 138

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.033461

研究报告

贵州主栽猕猴桃采后低温贮藏病害真菌多样性研究

  • 李辣梅 ,
  • 王瑞 ,
  • 余江平 ,
  • 雷霁卿 ,
  • 马超 ,
  • 李江阔 ,
  • 吴素芳 ,
  • 陆祥柳
展开
  • 1(贵阳学院 食品与制药工程学院,贵州 贵阳,550000)
    2(贵阳市果树技术推广站,贵州 贵阳,550000)
    3(国家农产品保鲜工程技术研究中心,天津,300384)
    4(修文县农业农村局,贵州 修文,550081)
第一作者:硕士研究生(王瑞教授为通信作者,E-mail:wangrui060729@126.com)

收稿日期: 2022-08-26

  修回日期: 2022-10-07

  网络出版日期: 2023-09-12

基金资助

贵州省创新平台建设计划(黔科中引地[2020]4018号);贵阳市科技计划项目(筑科合同[2021]3-24号);贵州省大学生创新创业训练计划项目(S202110976066)

收起

Analysis of fungal diversity in the major cultivated kiwifruit from Guizhou Province during postharvest low-temperature storage

  • LI Lamei ,
  • WANG Rui ,
  • YU Jiangping ,
  • LEI Jiqing ,
  • MA Chao ,
  • LI Jiangkuo ,
  • WU Sufang ,
  • LU Xiangliu
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  • 1(Food and Pharmaceutical Engineering Institute, Guiyang University, Guiyang 550000, China)
    2(Guiyang Fruit Tree Technology Extension Station, Guiyang 550000, China)
    3(Tianjin Key Laboratory of Postharvest Physiology and Storage of Agricultural Products, Tianjin 300384, China)
    4(Xiuwen County Agriculture Bureau, Xiuwen 550081, China)

Received date: 2022-08-26

  Revised date: 2022-10-07

  Online published: 2023-09-12

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摘要

由真菌引起的果实侵染病害造成猕猴桃产业的巨大损失。为探究贵州省主栽品种“贵长”“红阳”猕猴桃采后低温贮藏过程中的真菌群落多样性,该研究采用高通量测序技术比较不同产区猕猴桃真菌群落的组成,并结合花期、幼果期、采收期的降雨量,对采后贮藏侵染病害进行分析。结果表明,子囊菌门(Ascomycota)是优势菌门,葡萄孢属(Botrytis)、镰刀菌属(Fusarium)、间座壳属(Diaporthe)是主要优势属。主成分分析(principal component analysis, PCA)结果显示,贵州省“贵长”“红阳”真菌群落结构多样性较为相似。结合当地气象条件,推测贵州猕猴桃真菌多样性与降雨量有关,降雨量与猕猴桃果实真菌多样性成反比,与猕猴桃果实贮藏腐烂致病真菌的相对丰度成正比。当降雨量较大时,会提高果实侵染病害发生几率。该研究在贵州猕猴桃侵染病害防控、采后保鲜等领域提供理论依据。

关键词： 猕猴桃; 贮藏; 腐烂部位; 高通量; 真菌群落

本文引用格式

李辣梅 , 王瑞 , 余江平 , 雷霁卿 , 马超 , 李江阔 , 吴素芳 , 陆祥柳 . 贵州主栽猕猴桃采后低温贮藏病害真菌多样性研究[J]. 食品与发酵工业, 2023 , 49(16) : 131 -138 . DOI: 10.13995/j.cnki.11-1802/ts.033461

Abstract

Kiwifruit infection diseases caused by fungi contributing to significant industry losses. In order to explore the diversity of fungal communities in mainly cultivated of ‘Guichang’ and ‘Hongyang’ kiwifruits in Guizhou Province during low-temperature storage, the composition of kiwifruit fungal communities in different producing areas was compared by high-throughput sequencing technology, and the post-harvest infection diseases were analyzed in combination with the rainfall during flowering, young fruit and harvest periods. The results showed that Ascomycota was the dominant phylum, Botrytis, Fusarium, and Diaporthe were the main dominant genera. Principal component analysis results showed that ‘Guichang’ and ‘Hongyang’ cultivated in Guizhou Province had similar fungal community structures and diversity. Combined with local meteorological conditions, it should be presumed that the diversity of kiwifruit fungi in Guizhou Province is related to rainfall. Rainfall is inversely proportional to the diversity of kiwifruit fungi fruit, and is proportional to the relative abundance of decayed kiwifruit pathogenic fungi for storage. Heavy rainfall could increase the probability of kiwifruit infection. The findings of this study could provide theoretical support for kiwifruit infection control, postharvest storage, and other related fields in Guizhou Province.

Key words： kiwifruit(Actinidia chinensis); storage; rotten parts; high-throughput sequencing; fungal community

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