食品与发酵工业 >

2023 , Vol. 49 >Issue 16: 214 - 221

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.032376

研究报告

响应面优化橄榄仁红皮黄酮提取及组成分析

  • 徐莹 ,
  • 邹平 ,
  • 徐荣
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  • (常州大学 石油化工学院,江苏省绿色催化材料与技术重点实验室,江苏 常州,213164)
第一作者:硕士(邹平讲师和徐荣副教授为共同通信作者,E-mail:zouping@cczu.edu.cn;xurong@cczu.edu.cn)

收稿日期: 2022-05-18

  修回日期: 2022-06-27

  网络出版日期: 2023-09-12

基金资助

江苏省科技项目(BY2021215);常州市科技项目(CE20212030)

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Optimization of extraction and composition analysis of flavonoids from olive kernels by response surface

  • XU Ying ,
  • ZOU Ping ,
  • XU Rong
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  • (Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, School of Petrochemical Engineering, Changzhou University, Changzhou 213164, China)

Received date: 2022-05-18

  Revised date: 2022-06-27

  Online published: 2023-09-12

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摘要

以橄榄仁红皮为原材料,采用乙醇-水溶液提取活性较强的天然黄酮类化合物,通过单因素提取实验和响应面实验,得出最佳提取条件,以抗坏血酸为对照,对提取物进行抗氧化活性测定,并采用Thermo超高效液相色谱系统对提取的活性物质进行鉴定。结果表明,橄榄仁红皮黄酮类化合物(flavonoid from red skin of olive kernel, FRSOK)最佳的提取条件为浸提温度57.61 ℃,乙醇体积分数为75%,浸提时间为35 min,预测总黄酮含量243.53 μg/mL。天然的FRSOK清除羟自由基能力较强,清除DPPH自由基能力次之,清除超氧阴离子自由基能力最弱。通过分析以及对比,确定了橄榄仁红皮含有21种黄酮类化合物,包括新橙皮苷、槲皮素、山奈酚、异鼠李素等以及各自的衍生物。因此,FRSOK可作为抗氧化产品或其替代品应用到食品工业中,具有良好的应用前景。

关键词： 橄榄仁红皮; 黄酮类化合物; 抗氧化活性; 超高效液相色谱

本文引用格式

徐莹 , 邹平 , 徐荣 . 响应面优化橄榄仁红皮黄酮提取及组成分析[J]. 食品与发酵工业, 2023 , 49(16) : 214 -221 . DOI: 10.13995/j.cnki.11-1802/ts.032376

Abstract

Taking olive kernel red peel as raw material, ethanol-water solution was used to extract natural flavonoids with strong activity, and the optimal extraction conditions were obtained through one-factor extraction experiments and response surface experiments, and the antioxidant activity of the extract was determined by ascorbic acid as a control, and the extracted active substances were identified by thermo ultra-high performance liquid chromatography system. Results showed that the optimal extraction conditions for olive kernel red flavonoids (FRSOK) were an extraction temperature of 57.61 ℃, an ethanol volume fraction of 75%, an extraction time of 35 min, and a predicted total flavonoid content of 243.53 μg/mL. Natural FRSOK in ·OH scavenging ability was strong, followed by DPPH free radical scavenging ability, and ·O-2 scavenging ability was the weakest. Through analysis and comparison, it was determined that the red peel of olive kernel contained 21 kinds of flavonoids, including neohesperidin, quercetin, kaempferol, isorhamnetin, etc., and their respective derivatives. Therefore, FRSOK can be applied to the food industry as an antioxidant product or its substitute, with good application prospects.

Key words： olive red peel; flavonoids; antioxidant activity; ultra-high performance liquid chromatography

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