食品与发酵工业 >

2023 , Vol. 49 >Issue 16: 253 - 260

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.033570

研究报告

基于莲藕提取物生物合成纳米银及其抑菌活性研究

  • 吴承宗 ,
  • 魏亚楠 ,
  • 戴昕彤 ,
  • 苗雨颖 ,
  • 黄婷 ,
  • 赵康辉 ,
  • 王磊 ,
  • 宿红艳
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  • 1(鲁东大学 生命科学学院,山东 烟台,264025)
    2(鲁东大学 农学院,山东 烟台,264025)
第一作者:吴承宗(硕士)和魏亚楠(硕士)为共同第一作者(王磊教授和宿红艳教授为共同通信作者,E-mail:wanglei9909@163.com;suhongyan66@126.com)

收稿日期: 2022-09-07

  修回日期: 2022-10-12

  网络出版日期: 2023-09-12

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Biosynthesis of silver nanoparticles from lotus root extract and its antibacterial activity

  • WU Chengzong ,
  • WEI Yanan ,
  • DAI Xintong ,
  • MIAO Yuying ,
  • HUANG Ting ,
  • ZHAO Kanghui ,
  • WANG Lei ,
  • SU Hongyan
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  • 1(College of Life Sciences, Ludong University, Yantai 264025, China)
    2(College of Agriculture, Ludong University, Yantai 264025, China)

Received date: 2022-09-07

  Revised date: 2022-10-12

  Online published: 2023-09-12

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摘要

莲藕作为一种食药同源的食品,极具开发潜力。该研究利用莲藕提取液作为还原剂和稳定剂,采用一步法生物合成出纳米银抑菌剂,为莲藕的开发利用提供了一条新的途径。通过单因素试验探讨了AgNO3浓度、提取液用量、反应温度、反应时间等对纳米银制备的影响。优化后的合成产物经紫外-可见吸收光谱、透射电镜、X射线衍射分析等进行表征和鉴定,结果表明产物在426 nm附近有1个明显的纳米银等离子体子共振特征吸收峰,纳米银颗粒呈球形,平均粒径为8.2 nm,具有良好的分散性,为面心立方晶型。抑菌实验表明,莲藕生物合成纳米银与化学合成纳米相比具有更高的抑菌活性,对4种临床病原菌和4种水产病原菌均有显著的抑菌效果,对金黄色葡萄球菌的最小抑菌浓度和最小杀菌浓度分别为11.25、22.5 μg/mL。稳定性实验表明生物合成的纳米银具有良好的耐热稳定性和长期稳定性。综上所述,利用莲藕提取物可以生物合成较为理想的纳米银抑菌剂,有望在临床、水产等抑菌领域得到广泛应用。

关键词： 莲藕; 纳米银; 生物合成; 抑菌剂; 抑菌活性; 稳定性

本文引用格式

吴承宗 , 魏亚楠 , 戴昕彤 , 苗雨颖 , 黄婷 , 赵康辉 , 王磊 , 宿红艳 . 基于莲藕提取物生物合成纳米银及其抑菌活性研究[J]. 食品与发酵工业, 2023 , 49(16) : 253 -260 . DOI: 10.13995/j.cnki.11-1802/ts.033570

Abstract

Lotus root is a kind of medicinal and edible food with great development potential. Silver nanoparticles (AgNPs) antibacterial agent could be biosynthesized by one-step method using lotus root extract as reducing agent and stabilizer. The influences of AgNO3 concentration, volume of extraction solution, reaction temperature and time on the synthesis of AgNPs were investigated by single-factor experiment. The optimized synthetic product was confirmed by UV-visible spectra, transmission electron microscope and X-ray diffraction analysis. The results showed that the product had a strong plasmonon resonance absorption peak near 426 nm, which was the characteristic of AgNPs. The AgNPs were spherical with an average particle size of 8.2 nm. They had good dispersion and formed in a typical face-centered cubic structure. The antibacterial experiments showed that the biosynthesized AgNPs from lotus root had significant antibacterial effects on four clinical pathogens as well as four aquatic pathogens, which showed higher antibacterial activity compared with the chemical synthesis of AgNPs. The minimum inhibitory concentration and minimum bactericidal concentration of the AgNPs against Staphylococcus aureus was 11.25 and 22.5 μg/mL, respectively. The stability experiment showed that the biosynthesized AgNPs had excellent thermal stability and long-term stability. In conclusion, the lotus root extract can be used to biosynthesize ideal AgNPs antibacterial agent, which is expected to be widely used in clinical, aquatic and other antibacterial fields.

Key words： lotus root; silver nanoparticles; biosynthesis; antibacterial agent; antibacterial activity; stability

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