食品与发酵工业 >

2023 , Vol. 49 >Issue 16: 261 - 268

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.033611

分析与检测

花椒中代森联·吡唑醚菌酯的残留分析及膳食风险评估

  • 张耀海 ,
  • 赵其阳 ,
  • 王成秋 ,
  • 焦必宁
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  • 1(西南大学/中国农业科学院柑桔研究所,重庆,400712)
    2(农业农村部柑桔及苗木质量监督检测测试中心,重庆,400712)
    3(农业农村部柑橘类果品质量安全控制重点实验室,重庆,400712)
    4(农业农村部柑桔产品质量安全风险评估实验室,重庆,400712)
    5(国家柑桔工程技术研究中心,重庆,400712)
第一作者:博士,副研究员(通信作者,E-mail:zhangyaohai@cric.cn)

收稿日期: 2022-09-14

  修回日期: 2022-10-19

  网络出版日期: 2023-09-12

基金资助

重庆市技术创新与应用发展专项面上项目(cstc2021jscx-lyjsAX0007);农业农村部现代农业(柑橘)产业技术体系建设专项(CARS-26);国家重点研发计划项目(2019YFC1605604)

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Residue analysis and dietary risk assessment of metiramin and pyraclosrtrobin in Zanthoxylum bungeanum Maxim

  • ZHANG Yaohai ,
  • ZHAO Qiyang ,
  • WANG Chengqiu ,
  • JIAO Bining
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  • 1(Citrus Research Institute, Southwest University & Chinese Academy of Agricultural Sciences, Chongqing 400712, China)
    2(Quality Supervision and Testing Center for Citrus and Seedling, Ministry of Agriculture and Rural Affairs, Chongqing 400712, China)
    3(Key Laboratory of Quality and Safety Control of Citrus Fruits, Ministry of Agriculture and Rural Affairs, Chongqing 400712, China)
    4(Laboratory of Quality and Safety Risk Assessment for Citrus Products, Ministry of Agriculture and Rural Affairs, Chongqing 400712, China)
    5(National Citrus Engineering Research Center, Chongqing 400712, China)

Received date: 2022-09-14

  Revised date: 2022-10-19

  Online published: 2023-09-12

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摘要

该试验以花椒为研究对象,采用超高效液相色谱-串联质谱法和气相色谱-火焰光度检测器检测了代森联和吡唑醚菌酯及其代谢物乙撑硫脲在花椒生长发育过程中的残留量,研究了残留消解与转化规律,并探讨其可能产生的膳食健康风险。结果表明,建立的方法灵敏度及准确度良好,回收率为81%~107%,相对标准偏差为1.0%~9.5%,代森联、乙撑硫脲和吡唑醚菌酯的定量限均为0.05 mg/kg,能满足花椒中残留检测要求。按照良好农业规范在花椒上施用代森联和吡唑醚菌酯后,随着生长发育,代森联和吡唑醚菌酯均快速消解,施药28 d时鲜花椒消解率分别为95.3%~97.7%和94.1%~96.9%。鲜花椒加工成干花椒,能显著降低代森联在干花椒中的残留,而吡唑醚菌酯在干花椒中发生了显著的富集效应。花椒中代森联和吡唑醚菌酯急性和慢性膳食暴露风险均在可接受范围内。此研究结果可为我国花椒质量安全生产、监测和消费引导提供技术支撑和参考。

关键词： 花椒; 代森联; 吡唑醚菌酯; 残留分析; 膳食风险

本文引用格式

张耀海 , 赵其阳 , 王成秋 , 焦必宁 . 花椒中代森联·吡唑醚菌酯的残留分析及膳食风险评估[J]. 食品与发酵工业, 2023 , 49(16) : 261 -268 . DOI: 10.13995/j.cnki.11-1802/ts.033611

Abstract

In this experiment, Zanthoxylum bungeanum was used as the research object. The residues of metiramin, pyraclosrtrobin and its metabolite ethylene thiourea (ETU) in Zanthoxylum bungeanum during the growth and development were detected by ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS) and gas chromatography coupled with flame photometric detector (GC-FPD). Results showed that the sensitivity and accuracy of the established method were good, the mean recoveries were 81%-107%, the relative standard deviations (RSDs) were 1.0%-9.5%, and the limits of quantitation (LOQs) were 0.05 mg/kg, which could meet the requirements of residue detection in Zanthoxylum bungeanum. According to good agricultural practices, after the application of metiramin and pyraclosrtrobin on Zanthoxylum bungeanum, with the growth and development, both metiramin and pyraclosrtrobi were rapidly digested, and the digestibility rates of fresh Zanthoxylum bungeanum were 95.3%-97.7% and 94.1%-96.9%, respectively after 28 days of application. Processing fresh Zanthoxylum bungeanum into dry could significantly reduce the residue of metiramin in dry Zanthoxylum bungeanum, while pyraclosrtrobin had a significant enrichment effect in dry Zanthoxylum bungeanum. The residual levels of metiramin and pyraclosrtrobin in Zanthoxylum bungeanum were within the acceptable range of acute and chronic dietary exposure risks to human health. The research results provided technical support and reference for the quality and safety production, monitoring and consumption guidance of Zanthoxylum bungeanum in China.

Key words： Zanthoxylum bungeanum; metiramin; pyraclosrtrobin; residue analysis; dietary risk assessment

参考文献

[1] ZHANG M M, WANG J L, ZHU L, et al.Zanthoxylum bungeanum Maxim.(Rutaceae):A systematic review of its traditional uses, botany, phytochemistry, pharmacology, pharmacokinetics, and toxicology[J].International Journal of Molecular Sciences,2017, 18(10):2172-2203.
[2] 任媛媛, 翟晓巧, 张晨.我国花椒产业发展现状及存在问题和发展前景[J].河南林业科技, 2020, 40(4):31-34.
REN Y Y, ZHAI X Q, ZHANG C.Development status, problems and prospects of Zanthoxylum bungeanum industry in China[J].Journal of Henan Forestry Science and Technology, 2020, 40(4):31-34.
[3] KE J X, QU Y A, SHEN G H, et al.Edible quality and safety evaluation of Zanthoxylum from western China[C].International Conference Series:Materials Science and Engineering.2019.
[4] 吉波, 张耀华, 银航, 等.花椒病虫害生物防治的研究综述[J].陕西农业科学, 2020, 66(10):72-75;104.
JI B, ZHANG Y H, YIN H, et al.Review on biological control of Zanthoxylum bungeanum diseases and pests[J].Shaanxi Journal of Agricultural Sciences, 2020, 66(10):72-75;104.
[5] ZHOU J, DONG C, AN W J, et al.Dissipation of imidacloprid and its metabolites in Chinese prickly ash (Zanthoxylum) and their dietary risk assessment[J].Ecotoxicology and Environmental Safety, 2021, 225:112719.
[6] 唐光辉, 冯超, 冯俊涛, 等.戊唑醇在花椒体内的残留动态研究[J].西北林学院学报, 2013, 28(5):124-127.
TANG G H, FENG C, FENG J T, et al.Residue dynamics of tebuconazole in Chinese prickly ash tissues[J].Journal of Northwest Forestry University, 2013, 28(5):124-127.
[7] 范金平, 张盈, 魏进, 等.QuEChERS结合超高效液相色谱串联质谱法测定花椒中烯效唑残留量及贮藏稳定性[J].食品与发酵工业, 2021, 47(8):230-235.
FAN J P, ZHANG Y, WEI J, et al.Determination of uniconazole and storage stability in Zanthoxylum bungeanum Maxim by QuEChERS-UHPLC-MS/MS[J].Food and Fermentation Industries, 2021, 47(8):230-235.
[8] 杨双昱, 何志强, 曾全, 等.戊唑醇、肟菌酯和吡唑醚菌酯在青花椒中的残留行为[J].现代农药, 2022, 21(3):54-57.
YANG S Y, HE Z Q, ZENG Q, et al.Residual behavior of tebuconazole, trifloxystrobin and pyraclostrobin in Zanthoxylum schinifolium[J].Modern Agrochemicals, 2022, 21(3):54-57.
[9] SAMUELIAN S K, GREER L A, SAVOCCHIA S, et al.Application of Cabrio (a.i.pyraclostrobin) at flowering and veraison reduces the severity of bitter rot (Greeneria uvicola) and ripe rot (Colletotrichum acutatum) of grapes[J].Australian Journal of Grape and Wine Research.2014, 20(2):292-298.
[10] 曹欣然, 邹宗峰, 任强, 等.60%百泰对苹果主要病害的防治效果试验[J].落叶果树, 2015, 47(4):16-18.
CAO X R, ZOU Z F, REN Q, et al.Experiment on the control effect of 60% Baitai on main apple diseases[J].Deciduous Fruits, 2015, 47(4):16-18.
[11] 徐苏贵, 许焕明, 刘柳娇.60％百泰水分散粒剂防治柑橘疮痂病田间药效试验[J].广西植保, 2012, 25(3):6-8.
XU S G, XU H M, LIU L J. Field efficacy test of 60% Baitai water dispersible granule for controlling citrus scab[J]. Guangxi Plant Protection, 2012, 25(3): 6-8.
[12] 彭埃天, 宋晓兵, 习平根, 等.百泰对荔枝霜疫霉病的生物活性测定与防治试验[J].广东农业科学, 2009, 36(8):137-139.
PENG A T, SONG X B, XI P G, et al.Biological activity determination and control test of 60% Cabrio Top WG against Peronophythora litchii[J].Guangdong Agricultural Sciences, 2009, 36(8):137-139.
[13] 段瑞华, 韩方胜, 史明武, 等.百泰、Opera防治花生叶斑病田间药效试验[J].安徽农学通报, 2008, 14(17):204.
DUAN R H, HAN F S, SHI M W, et al. Field efficacy test of Baitai and Opera in controlling peanut leaf spot. Anhui Agricultural Science Bulletin, 2008, 14(17): 204.
[14] 曹琦, 董红强, 刘振亚, 等.百泰不同喷施浓度对棉花叶绿素、养分含量的影响[J].中国农学通报, 2018, 34(10):115-119.
CAO Q, DONG H Q, LIU Z Y, et al.Different spray concentrations of cabrio top:Effect on chlorophyll and nutrient content of cotton[J].Chinese Agricultural Science Bulletin, 2018, 34(10):115-119.
[15] ÇAYIR A, COŞKUN M. Genotoxicity of commercial fungicide Cabrio Plus on human cell. Cytotechnology, 2016, 68(5): 1697-1704.
[16] 李亮亮. 60%吡唑醚菌酯·代森联WG在大蒜及辣椒中的残留动态研究[D]. 泰安: 山东农业大学, 2013.
LI L L. Study on residual dynamics of Pyraclostrobin·Metiram60%WG in garlic and pepper[D]. Taian: Shandong Agricultural University, 2013.
[17] 张爱娟, 冯义志, 齐晓雪, 等. 代森联及其代谢物乙撑硫脲在番茄中的残留及膳食风险评估[J]. 农药科学与管理, 2020, 41(10): 17-23;28.
ZHANG A J, FENG Y Z, QI X X, et al. Residue and dietary risk assessment of metiram and its metabolite ethylene thiourea in tomato[J]. Pesticide Science and Administration, 2020, 41(10): 17-23;28.
[18] LI Z X, ZHANG Y H, ZHAO Q Y, et al. Determination, distribution and potential health risk assessment of insecticides and acaricides in citrus fruits of China. Journal of Food Composition and Analysis, 2022, 111: 104645.
[19] 常继乐, 王宇. 中国居民营养与健康状况监测2010—2013年综合报告[M]. 北京: 北京大学医学出版社, 2016: 61.
CHANG J L, WANG Y. Comprehensive Report on Nutrition and Health Status Monitoring of Chinese Residents from 2010 to 2013[M]. Beijing: Peking University Medical Press, 2016: 61.
[20] TAO X B, PENG W, YU X Q, et al. Determination of heavy metals in Chinese prickly ash from different production areas using inductively coupled plasma-mass spectrometry. Tropical Journal of Pharmaceutical Research, 2016, 15(8): 1767.
[21] 付卓锐, 欧亚非, 黄伊嘉, 等. 四川主产地花椒铅(Pb)累积特性及残留量检测与风险评估[J]. 四川林业科技, 2018, 39(1): 10-16.
FU Z R, OU Y F, HUANG Y J, et al. The accumulation characteristics, residues detection and risk assessment of lead(Pb) in Chinese prickly ash from main production areas in Sichuan[J]. Journal of Sichuan Forestry Science and Technology, 2018, 39(1): 10-16.
[22] 钟伟燕, 尹江伟, 李行方, 等. GC-FPD测定二硫化碳的计算方法及用于空气检测的探讨. 职业与健康, 2013, 29(15): 1903-1905;1908.
ZHONG W Y, YIN J W, LI X F, et al. Discussion on calculation method of carbon disulfide and determination in workplace air with GC-FPD. Occupation and Health, 2013, 29(15): 1903-1905;1908.
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