食品与发酵工业 >

2023 , Vol. 49 >Issue 17: 69 - 74

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.035709

研究报告

PCR与G-四链体联用可视化鉴别人参与西洋参

  • 刘墨祎 ,
  • 汪香君 ,
  • 汪洺卉 ,
  • 李迎 ,
  • 刘丽梅
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  • (北华大学 医学技术学院,吉林 吉林,132013)
第一作者:硕士研究生(刘丽梅副教授为通信作者,E-mail:liulm74@163.com)

收稿日期: 2023-04-03

  修回日期: 2023-05-03

  网络出版日期: 2023-09-27

基金资助

吉林省科技发展计划项目(20190304108YY);吉林省科技发展计划项目(YDZJ202201ZYTS515)

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Visual discrimination of Panax ginseng and Panax quinquefolium by PCR and G-quadruplex

  • LIU Moyi ,
  • WANG Xiangjun ,
  • WANG Minghui ,
  • LI Ying ,
  • LIU Limei
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  • (College of Medical Technology, Beihua University, Jilin 132013, China)

Received date: 2023-04-03

  Revised date: 2023-05-03

  Online published: 2023-09-27

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摘要

人参和西洋参作为我国常用名贵中药,其价值很高,由于它们为人参属近缘物种,因此二者在形态和化学成分方面较为相似。然而人参和西洋参的药性和药效有较大区别,二者在临床上不可混用,所以对二者进行鉴定就尤为重要。该研究基于人参与西洋参基因组DNA上的特异性snp位点,设计了5′端含有G-四链体互补序列的上下游引物,通过PCR扩增出大量含G-四链体的双链DNA,与氯高铁血红素(Hemin)结合形成具有过氧化物酶活性的DNA酶(DNAzyme),并在H2O2存在的情况下催化ABTS发生颜色变化。通过对PCR体系以及显色体系的优化,达到快速、简便地鉴别人参与西洋参,灵敏度可达到0.1 ng/μL。研究建立了针对人参和西洋参的PCR与G-四链体联用的可视化鉴别方法,为人参与西洋参的鉴别提供了新的途径。

关键词： PCR; G-四链体; 人参; 西洋参; 可视化鉴别

本文引用格式

刘墨祎 , 汪香君 , 汪洺卉 , 李迎 , 刘丽梅 . PCR与G-四链体联用可视化鉴别人参与西洋参[J]. 食品与发酵工业, 2023 , 49(17) : 69 -74 . DOI: 10.13995/j.cnki.11-1802/ts.035709

Abstract

Panax ginseng and Panax quinquefolium are commonly used as precious traditional Chinese medicines in China. Because they are related species of Panax ginseng, they are similar in morphology and chemical composition. However, the medicinal properties and efficacy of Panax ginseng and Panax quinquefolium are quite different, and they cannot be mixed in clinical practice, so it is particularly important to identify them. In this study, a large number of G-quadruplex-containing double-stranded DNA was amplified by PCR (polymerase chain reaction) with primers at the 5′ end of the specific snp site on the genomic DNA of Panax ginseng and Panax quinquefolium, which was denatured and combined with hemin to form a DNA enzyme with peroxidase activity (DNAzyme), catalyzed H2O2 and 2,2′-azino-bis (3-ethylbenzothiazoline-6sulfonic acid) diamine (ABTS) changes in color. Through the optimization of the PCR system and color development system, this study could quickly and simply identify Panax ginseng from Panax quinquefolium with a sensitivity of 0.1 ng/μL. A visual discrimination method of PCR and G-quadruplex for the identification of Panax ginseng and Panax quinquefolium was established, which provided a new way for human participation in the identification of Panax ginseng and Panax quinquefolium.

Key words： polymerase chain reaction (PCR); G-quadruplet; Panax ginseng; Panax quinquefolium; visual discrimination

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