该研究旨在探讨凝结芽孢杆菌CGMCC 9951对5%葡聚糖硫酸钠(dextran sulfate sodium, DSS)诱导的小鼠溃疡性结肠炎的干预效应。30只昆明小鼠随机分为对照组、模型组及CGMCC 9951低、中、高干预组,在适应性饲养7 d后,实验第1、2周对照组与模型组灌胃无菌生理盐水,低、中、高干预组分别灌胃3×106、3×107及3×108 CFU/mL CGMCC 9951菌悬液,实验第3周除对照组外每组给予5% DSS溶液进行造模,7 d后禁食12 h处死小鼠。与模型组相比,高剂量CGMCC 9951使小鼠体重增加8.59%,结肠长度增加38.55%;疾病活动指数降低53.33%,结肠炎性细胞浸润等黏膜损伤减轻46.43%;同时谷胱甘肽浓度升高181.89%、总超氧化物歧化酶活力升高48.73%、丙二醛含量下降55.41%,表明氧化应激程度得到改善;且促炎因子水平降低24.87%、抗炎因子水平提高46.81%,表明炎症反应的减轻;进一步研究发现,小鼠血清脂多糖水平降低16.57%,二胺氧化酶活力上升74.49%,有效维护了肠道机械屏障。该研究结果说明高剂量的CGMCC 9951对DSS诱导的小鼠结肠炎有良好的保护效果。
The purpose of this study was to investigate the intervention effect of Bacillus coagulans CGMCC 9951 on 5% dextran sodium sulfate (DSS)-induced ulcerative colitis in mice. Thirty KM mice were randomly divided into control group, model group and low, medium and high intervention groups of CGMCC 9951. After 7 d of adaptive feeding, the control group and model group were given 0.3 mL of sterile normal saline, and low, medium, and high intervention groups were given 3×106, 3×107, and 3×108 CFU/ mL of CGMCC 9951 bacterial suspension. In the third week of the experiment, each group except the control group was given 5% DSS solution for modeling. DSS was stopped at 7 d after modeling, and mice were sacrificed after fasting for 12 h. The results showed that compared with the model group, high dose CGMCC 9951 increased body weight by 8.59% and colon length by 38.55% in mice, decreased DAI by 53.33% and reduced mucosal damage such as colonic inflammatory cell infiltration by 46.43%. Moreover, glutathione concentration increased 181.89%, total superoxide dismutase activity increased 48.73% and malondialdehyde content decreased 55.41% indicating improvement in the degree of oxidative stress. Then, after intervention with GCMCC9951, a 24.87% decrease of pro-inflammatory cytokines and a 46.81% increase of anti-inflammatory cytokines indicated a reduction in the inflammatory response. Further studies showed that the concentration of lipopolysaccharide decreased by 16.57% and the activity of diamine oxidase increased by 74.49% in mice, which effectively maintained the colon barrier. The results showed that high dose of CGMCC 9951 had a good intervention effect on DSS-induced colitis in mice, and provided a scientific basis for the protection of B. coagulans CGMCC 9951 on colitis.
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