为探究增强Debaryomyces nepalensis控制冬枣采后病害的方法,采用果实及体外实验研究了CaCl2和D.nepalensis复合处理对冬枣采后黑斑病的控制效果和机制。结果表明,所选质量浓度为5、10、20、40 g/L的CaCl2对链格孢菌(Alternaria alternata)没有直接抑菌作用;5 g/L CaCl2结合1×108CFU/mL的D.nepalensis处理后,能够控制冬枣采后黑斑病,且控制效果显著强于D.nepalensis单独处理。5 g/L CaCl2结合1×108CFU/mL的D.nepalensis处理,能够显著增强D.nepalensis在冬枣果实伤口处的定殖能力,促进酵母生长并增强D.nepalensis生物膜形成能力。除此之外,CaCl2结合D.nepalensis处理后,冬枣果实苯丙氨酸解氨酶、肉桂酸-4-羟基化酶、4-香豆酸辅酶 A 连接酶和过氧化物酶活性增加,同时,总酚、类黄酮及木质素含量显著上升。综上,CaCl2通过增强D.nepalensis定殖能力及果实抗性提高D.nepalensis对冬枣采后黑斑病的控病能力。
In order to investigate the enhancement of Debaryomyces nepalensis for postharvest disease control of jujube, the effect and mechanism of combined treatment of CaCl2 and D. nepalensis on postharvest black spot disease control of jujube were studied by fruit and in vitro experiments. The results showed that the selected concentrations (5,10,20,40 g/L) of CaCl2 had no direct inhibitory effect against Alternaria alternata. However, the treatment of 5 g/L CaCl2 combined with 1×108 CFU/mL of D. nepalensis was able to control postharvest black spot disease of jujube, and the control effect was significantly better than that of D. nepalensis alone. The treatment of 5 g/L CaCl2 combined with 1×108 CFU/mL of D. nepalensis significantly enhanced the ability of D. nepalensis to colonize on the wound of jujube, promoted yeast growth and enhanced the biofilm formation of D. nepalensis. In addition, CaCl2 in combination with D. nepalensis treatment significantly enhanced the growth of yeast and biofilm formation of D. nepalensis. The activity of phenylalanine deaminase (PAL), cinnamate-4-hydroxylase (C4H), 4-coumaric acid coenzyme-A-ligase (4CL) and peroxidase (POD) increased, and total phenol, flavonoid and lignin contents increased significantly. In conclusion, CaCl2 improved the disease control capacity of D. nepalensis against postharvest black spot disease of jujube by enhancing the colonization ability and fruit resistance of D. nepalensis.
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