食品与发酵工业 >

2023 , Vol. 49 >Issue 20: 179 - 187

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.036293

研究报告

大鲵肽-硒螯合物对D-半乳糖致小鼠氧化应激损伤保护作用

  • 赵世博 ,
  • 刘俊霞 ,
  • 何琳琳 ,
  • 姜鹏飞 ,
  • 程虎 ,
  • 金文刚
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  • 1(陕西理工大学 生物科学与工程学院,秦巴生物资源与生态环境省部共建培育国家重点实验室,陕西 汉中,723001)
    2(陕西理工大学 陕西省资源生物重点实验室,陕西 汉中,723001)
    3(陕西理工大学 陕南秦巴山区生物资源综合开发协同创新中心,陕西 汉中,723001)
    4(大连工业大学 食品学院,国家海洋食品工程技术研究中心,辽宁 大连,116034)
    5(汉中市龙头山水产养殖开发有限公司,陕西 汉中,723001)
第一作者：硕士研究生(金文刚副教授为通信作者,E-mail:jinwengang@nwafu.edu.cn)

收稿日期: 2023-05-29

  修回日期: 2023-07-14

  网络出版日期: 2023-11-20

基金资助

陕西理工大学重点科研项目(SLG2106);汉中市青年科技创新团队支持计划(汉科[2019]26号)

收起

Protective effect of giant salamander peptides-selenium chelate on oxidative stress injury in mice caused by D-galactose

  • ZHAO Shibo ,
  • LIU Junxia ,
  • HE Linlin ,
  • JIANG Pengfei ,
  • CHENG Hu ,
  • JIN Wengang
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  • 1(State Key Laboratory of Qinba Biological Resources and Ecological Environment Jointly Built by Province and Ministry, College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723001, China)
    2(Shaanxi Key Laboratory of Bio-Resources, Shaanxi University of Technology, Hanzhong 723001, China)
    3(Collaborative Innovation Center for Comprehensive Development of Bio-Resources in Qinba Mountain Area of Southern Shaanxi, Shaanxi University of Technology, Hanzhong 723001, China)
    4(National Engineering Research Center for Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China)
    5(Hanzhong Dragon Mountain Aquaculture Development Co.Ltd., Hanzhong 723001, China)

Received date: 2023-05-29

  Revised date: 2023-07-14

  Online published: 2023-11-20

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摘要

为探究大鲵肽-硒螯合物的体内抗氧化活性,采用腹腔注射D-半乳糖建立氧化应激损伤小鼠模型,评价了不同剂量大鲵肽[100 mg/(kg·bw)和400 mg/(kg·bw)]及其肽-硒螯合物[10 μg/(kg·bw)和 30 μg/(kg·bw)]对小鼠氧化应激损伤的保护效应。结果显示,大鲵肽和大鲵肽-硒螯合物均具有较好的拮抗小鼠肝脏、脾脏和胸腺萎缩的作用。经不同剂量大鲵肽和大鲵肽-硒螯合物干预后,可使D-半乳糖诱导氧化应激损伤小鼠的血清、肝脏、心脏和脑组织中的总抗氧化能力、超氧化物歧化酶活力、谷胱甘肽含量、谷胱甘肽过氧化物酶活力显著增加(P<0.05),同时降低丙二醛和蛋白质羰基含量(P<0.05)。与大鲵肽相比,大鲵肽-硒螯合物对D-半乳糖致小鼠氧化应激损伤具有更好的保护作用,显示出更强的抗氧化活性。该研究为大鲵肽高附加值产品开发提供了一定的参考。

关键词： 大鲵; 肽-硒螯合物; 氧化应激; D-半乳糖; 抗氧化

本文引用格式

赵世博 , 刘俊霞 , 何琳琳 , 姜鹏飞 , 程虎 , 金文刚 . 大鲵肽-硒螯合物对D-半乳糖致小鼠氧化应激损伤保护作用[J]. 食品与发酵工业, 2023 , 49(20) : 179 -187 . DOI: 10.13995/j.cnki.11-1802/ts.036293

Abstract

To study the in vivo antioxidant activity of giant salamander peptide-selenium chelates, a mouse model of oxidative stress injury was established by intraperitoneal injection of D-galactose, the protective effects of different doses of peptides and their peptide-selenium chelates on oxidative stress injury in mice were evaluated. Results showed that both giant salamander peptides and their peptide-selenium chelates could antagonize the atrophy of the liver, spleen, and thymus in mice. After the intervention of different doses of giant salamander peptides and peptide-selenium chelates, the total antioxidant capacity, superoxide dismutase, glutathione, and glutathione peroxidase activities in the serum, liver, heart, and brain tissues of mice were significantly increased (P<0.05). Meanwhile, the contents of malondialdehyde and protein carbonyl groups were decreased (P<0.05). Compared with giant salamander peptides, their peptide-selenium chelates possessed a better protective effect on D-galactose-induced oxidative stress injury in mice and showed stronger antioxidant activity. This study provides valuable references for the development of value-added products based on giant salamander peptides.

Key words： giant salamander; peptide-selenium chelates; oxidative stress; D-galactose; antioxidation

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