As an important sulfur-containing amino acid, L-cysteine is widely used in food, medicine, cosmetics, and other industries. Efficient transporters play an important role in the construction of cell factories, they not only reduce feedback inhibition and cytotoxicity, but also improve cell production capacity. In this study, a novel L-cysteine exporter, Cg1298-Cg1299, was discovered in Corynebacterium glutamicum by transcriptome analysis. When 7.5 mmol/L L-cysteine was added, the cell growth of cg1298-cg1299 deletion strain was 24.2% lower than that of the control strain, while the complemented strain recovered its resistance to L-cysteine. In addition, the cysteine efflux capacity of Cg1298-Cg1299 was further examined by a dipeptide Cys-Tyr addition assay, and the results revealed that the efflux capacity of the cg1298-cg1299 deletion strain for L-cysteine decreased by 26.3% compared to the control strain. Finally, the production of L-cysteine increased by 17.1% by overexpression of cg1298-cg1299. In summary, our results showed that Cg1298-Cg1299 was a novel L-cysteine exporter in C. glutamicum, which significantly improved the tolerance of C. glutamicum to L-cysteine and the production of L-cysteine, providing an effective target for constructing efficient cell factory to produce L-cysteine in the future.
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