浓香型白酒糟水解液中含有高浓度的己酸,不经处理直接发酵会显著降低丁醇产量。该文研究了己酸对酒糟水解液发酵生产丁醇的影响,并结合层次分析法探索不同己酸浓度下的最优脱毒方法。结果表明,酒糟水解液中己酸质量浓度最高达到0.47 g/L,且随着己酸浓度的升高,其对丁醇发酵的抑制作用显著增强。超过0.2 g/L的己酸即会造成发酵过程中菌体量下降,糖利用率降低,丁醇和总溶剂产量显著下降。当己酸含量低于0.4 g/L时,经过生物炭处理的酒糟水解液的丁醇产量与对照组相当,且成本更低,是最优的己酸脱毒方法;而己酸含量达到0.6与1.1 g/L时,活性炭对己酸的去除率分别为80.0%和76.3%,远高于阴离子树脂与生物炭处理方法,是中、高浓度己酸最优的脱毒方式。该研究结果为白酒糟综合利用生产丁醇提供了参考。
Distillers' grain waste (DGW), the main waste in the Baijiu-making process, has been utilized as potential substrate for butanol production after an enzymatic hydrolysate process. However, large amounts of hexanoic acid contained in DGW enzymatic hydrolysate (DGWH) could significantly influence butanol fermentation. In this paper, the effect of hexanoic acid on butanol fermentation was studied, and the optimal detoxification methods under different hexanoic acid concentrations were explored by analytic hierarchy process. The results showed that hexanoic acid in DGWH could reach 0.47 g/L and the increase of hexanoic acid concentration would improve its inhibitory effect on butanol fermentation. Hexanoic acid above 0.2 g/L could result in the decrease of cell dry weight, sugar utilization, and the significant decrease of butanol and total solvent yield during fermentation. When the hexanoic acid content was below 0.4 g/L, butanol production of the biochar-treated DGWH was comparable to that of the control and the cost was relatively lower, which was considered as the optimal hexanoic acid detoxification method. When the hexanoic acid content reached 0.6 and 1.1 g/L, the removal rates of hexanoic acid by activated carbon were 80.0% and 76.3%, respectively, which were much higher than that of the anionic resin and biochar treatment, and was the best detoxification method. This study provides a reference for the comprehensive utilization of DGW to produce butanol.
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