In order to investigate the usage of endophytic fungus Diaporthe orixae sp. nov. from the Orixa japonica on the biosynthesis of silver nanoparticles and their catalytic activity, the endophytic fungus D. orixae was used in this study, the silver nanoparticles were synthesized by the hypha lysate, the synthesis conditions were optimized, the synthesized silver nanoparticles were characterized, and the catalytic activity of the synthesized silver nanoparticles was further investigated. The optimum conditions for the synthesis of silver nanoparticles in the hypha lysate of strain D. orixae were as follows: reaction time 12 h, strain biomass 5 g, AgNO3 concentration 2.0 mmol/L, pH 8, and 55 ℃. Scanning electron microscope results showed that the synthesized silver nanoparticles were spherical and had good dispersion; the X-ray diffraction pattern showed that the synthesized nano-silver crystal structure was face-centered cubic structure; the strain D. orixae was identified by Fourior transform infrared analysis self-carrying hydroxyl, carbonyl, amide bond, and other functional groups may contribute to the formation and stabilization of silver nanoparticles. In addition, the silver nanoparticles synthesized in this experiment have good catalytic activity for 4-nitrophenol, methyl orange and methylene blue. Endophytic fungus D. orixae the hypha lysate can be used for green synthesis of silver nanoparticles, and the synthesized silver nanoparticles have potential research value in green catalytic degradation of 4-nitrophenol and organic dyes.
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