食品与发酵工业 >

2023 , Vol. 49 >Issue 23: 307 - 314

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.034184

分析与检测

基于超高效液相色谱-三重四极杆质谱技术的冬瓜瓤化学成分分析

  • 陆胜勇 ,
  • 傅曼琴 ,
  • 徐玉娟 ,
  • 谢大森 ,
  • 余元善 ,
  • 温靖 ,
  • 肖更生
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  • 1(广东省农业科学院蚕业与农产品加工研究所/农业农村部功能食品重点实验室/广东省农产品加工重点实验室,广东 广州,510610)
    2(仲恺农业工程学院 轻工食品学院,广东 广州,510225)
    3(广东省农业科学院蔬菜研究所/广东省蔬菜新技术研究重点实验室,广东 广州,510640)
第一作者:硕士研究生(肖更生研究员为通信作者,E-mail:guoshuxgs@163.com )

收稿日期: 2022-11-02

  修回日期: 2022-11-23

  网络出版日期: 2024-01-02

基金资助

广东省农业科学院食品营养与健康研究中心建设运行经费(XTXM202205);广东省现代农业产业技术体系创新团队建设项目(2022KJ110);广东省农业科学院“十四五”农业优势产业学科团队(202109TD);广东省农业科学院人才项目(R2020PY-JX011)

收起

Chemical composition analysis of wax gourd pith based on ultra-performance liquid chromatography-triple quadrupole mass spectrometry technology

  • LU Shengyong ,
  • FU Manqin ,
  • XU Yujuan ,
  • XIE Dasen ,
  • YU Yuanshan ,
  • WEN Jing ,
  • XIAO Gengsheng
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  • 1(Sericultural & Agri-Food Research Institute Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, China)
    2(Zhongkai College of Agricultural Engineering of Light Industry Food Institute, Guangzhou 510225, China)
    3(Vegetable Research Institute, Guangdong Academy of Agricultural Sciences/Key Laboratory fog New Technology Research of Vegetables, Guangzhou 510640, China)

Received date: 2022-11-02

  Revised date: 2022-11-23

  Online published: 2024-01-02

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摘要

采用超高效液相色谱-串联质谱的广泛代谢组学技术对冬瓜瓤的代谢物进行分离和鉴定,分析和描述冬瓜瓤的代谢物组成和丰度的信息。通过测定冬瓜皮、肉、瓤和籽4个部位的总黄酮、总酚及抗氧化能力发现,冬瓜瓤的总黄酮含量仅次于冬瓜肉,总酚含量则最高,抗氧化能力最强。因此,该研究选取冬瓜瓤作为研究对象,分析其化学成分,结果显示,冬瓜瓤中共分离鉴定出代谢物825个,初生代谢物451个(59.01%),次生代谢物374个(40.99%),其中氨基酸及其衍生物(114个,21.41%)、脂质(123个,13.28%)、有机酸(74个,10.82%)、糖及醇类(58个,6.68%)、核苷酸及其衍生物(62个,5.62%)、维生素(20个,1.22%)、酚酸类(161个,21.10%)、生物碱(57个,7.84%)、黄酮(92个,7.36%)、木脂素和香豆素(51个,3.63%)、萜类(13个,1.06%)。冬瓜瓤各类代谢物种类丰富,富集程度高,还具有显著的营养功能、活性作用及药效,其中相对含量≥0.5%的代谢物对冬瓜副产物营养和活性有重要贡献,该研究结果为冬瓜瓤副产物精深加工和利用提供理论依据。

关键词： 冬瓜瓤; 广泛靶向代谢组学; 化学成分; 营养物质; 功能活性

本文引用格式

陆胜勇 , 傅曼琴 , 徐玉娟 , 谢大森 , 余元善 , 温靖 , 肖更生 . 基于超高效液相色谱-三重四极杆质谱技术的冬瓜瓤化学成分分析[J]. 食品与发酵工业, 2023 , 49(23) : 307 -314 . DOI: 10.13995/j.cnki.11-1802/ts.034184

Abstract

The metabolites from wax gourd pith were separated and identified by ultra-high performance liquid chromatography-tandem mass spectrometry-based extensive metabolomics technology and the information about the composition and abundance of metabolites in wax gourd pith was analyzed and described. By measuring the total flavonoids, total phenols, and antioxidant capacity of four parts of wax gourd skin, pulp, pith, and seed, it was found that the total flavone content of wax gourd pith was second only to that of wax gourd pulp, while the wax gourd pith had the highest total phenol content and the strongest antioxidant capacity. Therefore, the wax gourd pith was selected as the research object to analyze its chemical composition. Results showed that a total of 825 metabolites were isolated and identified from the pith of wax gourd, including 451 (59.01%) primary metabolites and 374 (40.99%) secondary metabolites, including amino acids and their derivatives (114, 21.41%), lipids (123, 13.28%), organic acids (74, 10.82%), sugars and alcohols (58, 6.68%), nucleotides and their derivatives (62, 5.62%), vitamins (20, 1.22%), phenolic acids (161, 21.10%), alkaloids (57, 7.84%), flavonoids (92, 7.36%), lignans and coumarins (51, 3.63%), and terpenes (13, 1.06%). The flesh of wax gourd was rich in various metabolites with high abundance and also had significant nutritional function, active effect and efficacy. The metabolites with relative content ≥0.5% had an important contribution to the nutrition and activity of wax gourd by-products. The results of this study provide a theoretical basis for the deep processing and utilization of wax gourd pulp by-products and improve the added value and economic benefits of the wax gourd industry.

Key words： wax gourd pith; widely targeted metabolomics; chemical composition; nutrients; functional activity

参考文献

[1] XIE D S, XU Y C, WANG J P, et al.The wax gourd genomes offer insights into the genetic diversity and ancestral cucurbit karyotype[J].Nature Communications, 2019, 10:5158.
[2] ZAINI N A M, ANWAR F, HAMID A A, et al.Kundur[Benincasa hispida (Thunb.) Cogn.]:A potential source for valuable nutrients and functional foods[J].Food Research International, 2011, 44(7):2368-2376.
[3] MA L L, LIU Z G, CHENG Z K, et al.Identification and application of BhAPRR2 controlling peel colour in wax gourd (Benincasa hispida)[J].Frontiers in Plant Science, 2021, 12:716772.
[4] 张晓蕾, 沈国明, 杜琪珍.DEHP在冬瓜果皮和果肉的累积差异及与果皮蜡质组成的关系[J].北方园艺, 2011(16):37-40.
ZHANG X L, SHEN G M, DU Q Z.The differences of DEHP accumulation in wax gourd peel and pulp and relationship with waxy composition in peel[J].Northern Horticulture, 2011(16):37-40.
[5] 盛留洋, 翁佩芳, 吴祖芳, 等.生腌熟腌冬瓜挥发性风味成分及指纹图谱分析[J].中国野生植物资源, 2018, 37(6):19-27.
SHENG L Y, WENG P F, WU Z F, et al.Analysis of volatile components and fingerprint of raw and cooked pickled wax gourd[J].Chinese Wild Plant Resources, 2018, 37(6):19-27.
[6] 李杨, 刘明杰, 肖洪, 等.不同干燥工艺对冬瓜皮中生物活性成分含量的影响[J].中国调味品, 2018, 43(4):53-58.
LI Y, LIU M J, XIAO H, et al.Effects of different drying techniques on the bioactive components content in waxgourd peel[J].China Condiment, 2018, 43(4):53-58.
[7] 童军茂, 周红, 杨艳彬, 等.冬瓜复合汁加工技术的研究[J].食品与发酵工业, 2001, 27(12):77-79.
TONG J M, ZHOU H, YANG Y B, et al.Study on processing technology of wax gourd compound juice[J].Food and Fermentation Industries, 2001, 27(12):77-79.
[8] 陈占伟, 高映菊, 陈广泉, 等.冬瓜酸乳饮料生产工艺优化研究[J].甘肃农业科技, 2022, 53(3):64-70.
CHEN Z W, GAO Y J, CHEN G Q, et al.Study on the optimization of production technology of wax melon sour milk beverage[J].Gansu Agricultural Science and Technology, 2022, 53(3):64-70.
[9] 陈月华, 李嘉, 符锋, 等.不同干燥方法对冬瓜皮活性成分的影响[J].食品与发酵工业, 2017, 43(2):129-133.
CHEN Y H, LI J, FU F, et al.Effects of different drying methods on the content of active components of wax gourd peel[J].Food and Fermentation Industries, 2017, 43(2):129-133.
[10] 刘宗林, 王熊, 郭宏.冬瓜浓缩液-冬瓜综合利用之一[J].食品科学, 2000, 21(12):51-53.
LIU Z L, WANG X, GUO H.Study on the comprehensive use of the concentrated juice of benincare hispida[J].Food Science, 2000, 21(12):51-53.
[11] 武杰, 张引成, 项海波, 等.冬瓜瓤汁保健饮料配方优化研究[J].农产品加工, 2012(7):74-76.
WU J, ZHANG Y C, XIANG H B, et al.Study on formula optimization of health beverage of wax gourd pith juice[J].Farm Products Processing, 2012(7):74-76.
[12] 王立抗, 陈鸿庚, 黄智霖, 等.牛大力不同部位总黄酮、多酚含量及其抗氧化活性研究[J].中华中医药学刊, 2022, 40(3):139-142.
WANG L K, CHEN H G, HUANG Z L, et al.Antioxidant activities and contents of total flavonoids and polyphenols from different parts of Niudali (Millettia speciose Champ)[J].Chinese Archives of Traditional Chinese Medicine, 2022, 40(3):139-142.
[13] DINARDO A, SUBRAMANIAN J, SINGH A.Investigation of antioxidant content and capacity in yellow European plums[J].International Journal of Fruit Science, 2018, 18(1):99-116.
[14] SHARMA O P, BHAT T K.DPPH antioxidant assay revisited[J].Food Chemistry, 2009, 113(4):1202-1205.
[15] MZID M, BEN KHEDIR S, BEN SALEM M, et al.Antioxidant and antimicrobial activities of ethanol and aqueous extracts from Urtica urens[J].Pharmaceutical Biology, 2017, 55(1):775-781.
[16] CHEN W, GONG L, GUO Z L, et al.A novel integrated method for large-scale detection, identification, and quantification of widely targeted metabolites:Application in the study of rice metabolomics[J].Molecular Plant, 2013, 6(6):1769-1780.
[17] ZHOU Y Q, SHAO L Y, ZHU J L, et al.Comparative analysis of tuberous root metabolites between cultivated and wild varieties of Rehmannia glutinosa by widely targeted metabolomics[J].Scientific Reports, 2021, 11:11460.
[18] PALIKHOVA T A.Effects of monodiets with different tryptophan contents on shell color, behavior, and neuron activity in the common snail[J].Neuroscience and Behavioral Physiology, 2021, 51(5):616-619.
[19] RAVIPATI A S, ZHANG L, KOYYALAMUDI S R, et al.Antioxidant and anti-inflammatory activities of selected Chinese medicinal plants and their relation with antioxidant content[J].BMC Complementary and Alternative Medicine, 2012, 12:173.
[20] COMAI S, BERTAZZO A, BRUGHERA M, et al.Tryptophan in health and disease[J].Advances in Clinical Chemistry, 2020, 95:165-218.
[21] YU Z L, JIANG H R, GUO R C, et al.Taste, umami-enhance effect and amino acid sequence of peptides separated from silkworm pupa hydrolysate[J].Food Research International, 2018, 108:144-150.
[22] 董长勇, 于伟厚, 苟亚夫, 等.核苷酸类食品添加剂的生产与应用研究进展[J].食品与发酵工业, 2022, 48(22):345-352.
DONG C Y, YU W H, GOU Y F, et al.Research progress on the production and application of nucleotide-based food additives[J].Food and Fermentation Industries, 2022, 48(22):345-352.
[23] 由玉卿, 秦智伟, 周秀艳, 等.黄瓜果实中丙醇二酸含量的配合力分析[J].北方园艺, 2014(10):19-21.
YOU Y Q, QIN Z W, ZHOU X Y, et al.Analysis on combining ability of tartronic acid content in cucumber[J].Northern Horticulture, 2014(10):19-21.
[24] 郑亚琴. 仙人掌黄瓜复合饮料的研制[J].食品科学, 2010, 31(22):525-528.
ZHENG Y Q.Development of compound beverage from cactus and cucumber[J].Food Science, 2010, 31(22):525-528.
[25] JANDACEK R J.Linoleic acid:A nutritional quandary[J].Healthcare, 2017, 5(2):25.
[26] ALONSO-CASTRO A J, SERRANO-VEGA R, PÉREZ GUTIÉRREZ S, et al.Myristic acid reduces skin inflammation and nociception[J].Journal of Food Biochemistry, 2022, 46(1):e14013.
[27] 张梦玲, 李绮丽, 李高阳, 等.柑橘多甲氧基黄酮的来源及生物学作用[J].食品与机械, 2019, 35(1):213-220;236.
ZHANG M L, LI Q L, LI G Y, et al.Sources and biological functions of citrus polymethoxy flavones[J].Food & Machinery, 2019, 35(1):213-220;236.
[28] LAI C S, WU J C, HO C T, et al.Disease chemopreventive effects and molecular mechanisms of hydroxylated polymethoxyflavones[J].BioFactors, 2015, 41(5):301-313.
[29] LEE M S, LEE H J, LEE H S, et al.L-carnitine stimulates lipolysis via induction of the lipolytic gene expression and suppression of the adipogenic gene expression in 3T3-L1 adipocytes[J].Journal of Medicinal Food, 2006, 9(4):468-473.
[30] ZEISEL S H, KLATT K C, CAUDILL M A.Choline[J].Advances in Nutrition, 2018, 9(1):58-60.
[31] BLUSZTAJN J K, SLACK B E, MELLOTT T J.Neuroprotective actions of dietary choline[J].Nutrients, 2017, 9(8):815.
[32] CASTAÑEDA R, RODRIGUEZ I, NAM Y H, et al.Trigonelline promotes auditory function through nerve growth factor signaling on diabetic animal models[J].Phytomedicine, 2017, 36:128-136.
[33] BAKURADZE T, LANG R, HOFMANN T, et al.Antioxidant effectiveness of coffee extracts and selected constituents in cell-free systems and human colon cell lines[J].Molecular Nutrition & Food Research, 2010, 54(12):1734-1743.
[34] HIRAKAWA N, OKAUCHI R, MIURA Y, et al.Anti-invasive activity of niacin and trigonelline against cancer cells[J].Bioscience, Biotechnology, and Biochemistry, 2005, 69(3):653-658.
[35] RASHMI H B, NEGI P S.Phenolic acids from vegetables:A review on processing stability and health benefits[J].Food Research International, 2020, 136:109298.
[36] BANERJEE G, CHATTOPADHYAY P.Vanillin biotechnology:The perspectives and future[J].Journal of the Science of Food and Agriculture, 2019, 99(2):499-506.
[37] FANG X H, HU X D.Advances in the synthesis of lignan natural products[J].Molecules, 2018, 23(12):3385.
[38] SRIKRISHNA D, GODUGU C, DUBEY P K.A review on pharmacological properties of coumarins[J].Mini Reviews in Medicinal Chemistry, 2018, 18(2):113-141.
[39] DEY P, SOSMITHA G, ELINA K, et al.An overview of the pharmacological activities of scopoletin against different chronic diseases[J].Pharmacological Research, 2022:106202.
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