食品与发酵工业 >

2023 , Vol. 49 >Issue 24: 132 - 139

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.033521

研究报告

滩羊尾脂共轭亚油酸的提取及其生物活性研究

  • 裴慧敏 ,
  • 李亚蕾 ,
  • 曹松敏
展开
  • (宁夏大学 食品与葡萄酒学院,宁夏 银川,750021)
第一作者:硕士研究生(曹松敏讲师为通信作者,E-mail:songmin_cao@126.com)

收稿日期: 2022-09-02

  修回日期: 2022-10-08

  网络出版日期: 2024-01-17

基金资助

宁夏自然科学基金项目(2022AAC03078);河北省重点研发计划项目(21327121D)

收起

Study on extraction and bioactivity of conjugated linoleic acid from Tan sheep tail fat

  • PEI Huimin ,
  • LI Yalei ,
  • CAO Songmin
Expand
  • (School of Food & Wine, Ningxia University, Yinchuan 750021, China)

Received date: 2022-09-02

  Revised date: 2022-10-08

  Online published: 2024-01-17

Fold

摘要

该研究以滩羊尾为原料,低温结晶联合尿素包合为方法,共轭亚油酸(conjugated linoleic acid, CLA)得率和纯度为评价指标,在单因素基础上结合正交优化得出最佳提取工艺条件,并探究了滩羊尾脂CLA的生物活性。结果表明,采用低温结晶法提取滩羊尾脂CLA得到的最优条件为:提取溶剂为无水乙醇,结晶温度-20 ℃,结晶时间12 h,混合脂肪酸与无水乙醇料液比1∶12(g∶mL),提取纯度为(18.62±0.51) mg/g。在此基础上采用尿素包合法,在包合温度25 ℃,包合时间14 h,脂肪酸与尿素质量比1∶2,尿素与乙醇料液比1∶5(g∶mL)条件下效果最优,其纯度为(28.23±2.36) mg/g。体外抗氧化指标结果显示,DPPH自由基和ABTS阳离子自由基的清除率随滩羊尾脂CLA质量浓度的增加而增加,在质量浓度为6 mg/mL时,清除率分别达到(35.94±4.07)%和(51.76±5.57)%。降胆固醇活性指标结果显示,胆固醇胶束抑制率为(60.33±5.88)%,对牛磺胆酸钠、甘氨胆酸钠和胆酸钠的结合率分别为(16.11±4.01)%、(37.56±3.17)%和(25.11±4.82)%。该结果可为滩羊尾脂CLA工业化提取及活性应用方面的研究提供参考依据。

关键词： 滩羊尾脂; 共轭亚油酸; 低温结晶; 尿素包合; 抗氧化活性; 降胆固醇活性

本文引用格式

裴慧敏 , 李亚蕾 , 曹松敏 . 滩羊尾脂共轭亚油酸的提取及其生物活性研究[J]. 食品与发酵工业, 2023 , 49(24) : 132 -139 . DOI: 10.13995/j.cnki.11-1802/ts.033521

Abstract

In this paper, Tan sheep tail was used as raw material, low-temperature crystallization combined with urea inclusion as the method, and the yield and purity of conjugated linoleic acid (CLA) were used as evaluation indicators. The extraction process conditions were investigated, and the biological activity of Tan sheep tail fat CLA was explored. Results showed that the optimal conditions for extracting CLA from Tan sheep tail fat by low-temperature crystallization method were that the extraction solvent was absolute ethanol, the crystallization temperature was -20 ℃, the crystallization time was 12 h, and the ratio of mixed fatty acids to anhydrous ethanol was 1∶12 (g∶mL), the purity was (18.62±0.51) mg/g. On this basis, the urea inclusion method was adopted, when the inclusion temperature was 25 ℃, the inclusion time was 14 h, the mass ratio of fatty acid to urea was 1∶2, and the mass ratio of urea to ethanol was 1∶5 (g∶mL), the effect was best, and the purity was (28.23±2.36) mg/g. The results of in vitro antioxidant indicators showed that the scavenging rates of DPPH radical and ABTS cationic radicals increased with the increase of CLA concentration in Tan sheep tail fat. When the mass concentration was 6 mg/mL, the scavenging rates reached (35.94±4.07)% and (51.76±5.57)%. The results of cholesterol-lowering activity indicators showed that the inhibition rate of cholesterol micelles was (60.33±5.88)%, and the binding rates to sodium taurocholate, sodium glycocholate, and sodium cholate were (16.11±4.01)%, (37.56±3.17)%, and (25.11±4.82)%, respectively. The results provide a reference for future research on the industrial extraction and active application of Tan sheep tail fat CLA.

Key words： Tan sheep tail fat; conjugated linoleic acid; cryogenic crystallization; urea inclusion; antioxidant activity; cholesterol-lowering activity

参考文献

[1] 马梦斌, 罗瑞明, 李亚蕾.基于矿物元素指纹差异的不同产地滩羊肉判别[J].食品科学, 2020, 41(6):316-321.
MA M B, LUO R M, LI Y L.Discrimination of different geographical origins of Tan sheep meat based on mineral element fingerprints[J].Food Science, 2020, 41(6):316-321.
[2] 于洋, 逄晓阳, 岳彩娟, 等.宁夏滩羊羊尾油手工皂生产工艺优化研究[J].宁夏农林科技, 2020, 61(9):57-59;66.
YU Y, PANG X Y, YUE C J, et al.Optimization of production technology of Tan sheep′s oil soap[J].Ningxia Journal of Agriculture and Forestry Science and Technology, 2020, 61(9):57-59;66.
[3] ALBERS R, VAN DER WIELEN R P J, BRINK E J, et al.Effects of cis-9, trans-11 and trans-10, cis-12 conjugated linoleic acid (CLA) isomers on immune function in healthy men[J].European Journal of Clinical Nutrition, 2003, 57(4):595-603.
[4] ZHAO W S, ZHAI J J, WANG Y H, et al.Conjugated linoleic acid supplementation enhances antihypertensive effect of ramipril in Chinese patients with obesity-related hypertension[J].American Journal of Hypertension, 2009, 22(6):680-686.
[5] CHUJO H, YAMASAKI M, NOU S, et al.Effect of conjugated linoleic acid isomers on growth factor-induced proliferation of human breast cancer cells[J].Cancer Letters, 2003, 202(1):81-87.
[6] HUANG Y S, LIN Z D, RONG H A, et al.Effects of conjugated linoleic acid on growth, body composition, antioxidant status, lipid metabolism and immunity parameters of juvenile Chu′s croaker, Nibea coibor[J].Aquaculture Research, 2018, 49(1):546-556.
[7] DEN HARTIGH L J, HAN C Y, WANG S R, et al.10E,12Z-conjugated linoleic acid impairs adipocyte triglyceride storage by enhancing fatty acid oxidation, lipolysis, and mitochondrial reactive oxygen species[J].Journal of Lipid Research, 2013, 54(11):2964-2978.
[8] IP C, DONG Y, IP M M, et al.Conjugated linoleic acid isomers and mammary cancer prevention[J].Nutrition and Cancer, 2002, 43(1):52-58.
[9] 刘芸. 黄秋葵籽油中共轭亚油酸的制备及其抗氧化活性的研究[D].杭州:浙江大学, 2016.
LIU Y.Preparation of conjugated linoleic acid from okra seed oil and the study of its anti-oxidant activity[D].Hangzhou:Zhejiang University, 2016.
[10] 赵俊. 茶油制备共轭亚油酸及其抗氧化活性的研究[D].广州:华南理工大学, 2019.
ZHAO J.Preparation of conjugated linoleic acid from Camellia oleifera seed oil and its antioxidant activity[D].Guangzhou:South China University of Technology, 2019.
[11] 李垚, 徐尔尼, 杨欣, 等.嗜酸乳杆菌转化菜籽油生成共轭亚油酸条件的优化[J].食品与发酵工业, 2013, 39(3):32-37.
LI Y, XU E N, YANG X, et al.Optimization of the conditions for conversion of colza oil into conjugated linoleic acid by rapeseed oil by Lactobacillus acidophilus[J] Food and Fermentation Industries, 2013, 39(3):32-37.
[12] BESSA R J B, SANTOS-SILVA J, RIBEIRO J M R, et al.Reticulo-rumen biohydrogenation and the enrichment of ruminant edible products with linoleic acid conjugated isomers[J].Livestock Production Science, 2000, 63(3):201-211.
[13] 于洋, 冯毅, 宋宏立, 等.短程分子蒸馏提取滩羊尾脂共轭亚油酸的研究[J].食品研究与开发, 2020, 41(19):1-7.
YU Y, FENG Y, SONG H L, et al.Study on extraction of conjugated linoleic acid from Tan sheep tail lipid by short-range molecular distillation[J].Food Research and Development, 2020, 41(19):1-7.
[14] 李慧美. 黄油中共轭亚油酸对DMBA诱导小鼠乳腺癌的抑制作用及机理研究[D].南昌:南昌大学, 2019.
LI H M.Inhibitory effect and mechanism of conjugated linoleic acid in butter on DMBA-induced breast cancer in mice[D].Nanchang:Nanchang University, 2019.
[15] MA D W L, WIERZBICKI A A, FIELD C J, et al.Preparation of conjugated linoleic acid from safflower oil[J].Journal of the American Oil Chemists′ Society, 1999, 76(6):729-730.
[16] LAI P, LI K Y, LU S, et al.Phytochemicals and antioxidant properties of solvent extracts from Japonica rice bran[J].Food Chemistry, 2009, 117(3):538-544.
[17] 高婕. 具有降胆固醇活性的亚麻籽肽的制备及其对大鼠降胆固醇作用的研究[D].呼和浩特:内蒙古农业大学, 2018.
GAO J.A novel hypocholesterolemic peptide derived from flaxseed protein and its effect on hyperlipidemic rats[D].Hohhot:Inner Mongolia Agricultural University, 2018.
[18] 刘晓静. 亚麻籽肽降胆固醇作用的研究[D].呼和浩特:内蒙古农业大学, 2020.
LIU X J.Study on the cholesterol-lowering effect of flaxseed peptide[D].Hohhot:Inner Mongolia Agricultural University, 2020.
[19] MORALES-MEDINA R, DE LEÓN G, MUNIO M, et al.Mass transfer modeling of sardine oil polyunsaturated fatty acid (PUFA) concentration by low temperature crystallization[J].Journal of Food Engineering, 2016, 183:16-23.
[20] 安腾奇, 成取林, 余成山, 等.溶剂结晶和尿素包合法提高油酸纯度的研究[J].中国油脂, 2016, 41(9):64-68.
AN T Q,CHENG Q L,YU C S,et al.Improvement of oleic acid purity by solvent crystallization and urea adduction[J].China Oils and Fats, 2016, 41(9):64-68.
[21] 曾俊, 岳杨, 任小娜, 等.尿素包合法从葡萄籽油中富集高纯度亚油酸甲酯的研究及包合物表征分析[J].中国油脂,2022,47(12):25-30.
ZENG J, YUE Y, REN X N, et al.Preparation of high purity methyl linoleate from grape seed oil by urea adduction and characterization of adduction complexes[J].China Oil and Fat, 2022,47(12):25-30.
[22] 吴明一. 尿素包合法纯化不饱和脂肪酸的研究[D].天津:天津大学, 2007.
WU M Y.Research on purification of unsaturated fatty acids by urea inclusion fractionation[D].Tianjin:Tianjin University, 2007.
[23] 郭莹莹, 刘玉兰, 梁绍全, 等.尿素包合法富集文冠果油中神经酸的研究[J].中国油脂, 2018, 43(7):119-123.
GUO Y Y, LIU Y L, LIANG S Q, et al.Enrichment of nervonic acid from Xanthoceras sorbifolia Bunge.oil by urea inclusion fractionation[J].China Oils and Fats, 2018, 43(7):119-123.
[24] 孙潇辉. 瓜蒌籽油提取及多不饱和脂肪酸的富集与微胶囊制备[D].合肥:安徽农业大学, 2013.
SUN X H.The extraction of Trichosanthes kirilowii Maxim.seed oil and the enrichment and encapsulation of polyunsaturated fatty acid[D].Hefei:Anhui Agricultural University, 2013.
[25] 王晓宇, 杜国荣, 李华.抗氧化能力的体外测定方法研究进展[J].食品与生物技术学报, 2012, 31(3):247-252.
WANG X Y, DU G R, LI H.Progress of analytical methods for antioxidant capacity in vitro[J].Journal of Food Science and Biotechnology, 2012, 31(3):247-252.
[26] 侯建军, 陈存社, 张甜甜, 等.羊腰部脂肪中共轭亚油酸提取及热稳定性[J].食品科学技术学报, 2013, 31(1):60-63;82.
HOU J J, CHEN C S, ZHANG T T, et al.Study on extraction and thermal stability of conjugated linoleic acid from sheep waist fat[J].Journal of Food Science and Technology, 2013, 31(1):60-63;82.
[27] 陈丽敏. 山核桃亚油酸分离及共轭亚油酸制备技术研究[D].合肥:安徽农业大学, 2013.
CHEN L M.Study on the separation of linoleic acid in Carya cathayensis Sarg.and synthesis technology of conjugated linoleic acids[D].Hefei:Anhui Agricultural University, 2013.
[28] KIM Y J, LEE K W, LEE H J.Total antioxidant capacity of arginine-conjugated linoleic acid (CLA) complex[J].Journal of Agricultural and Food Chemistry, 2004, 52(3):439-444.
[29] LEE K N, KRITCHEVSKY D, PARIZAA M W.Conjugated linoleic acid and atherosclerosis in rabbits[J].Atherosclerosis, 1994, 108(1):19-25.
[30] KRITCHEVSKY D, TEPPER S A, WRIGHT S, et al.Conjugated linoleic acid isomer effects in atherosclerosis:Growth and regression of lesions[J].Lipids, 2004, 39(7):611-616.
[31] 廖坤梅, 白天禾, 陈楚华, 等.高良姜粗多糖体外降胆固醇效果研究[J].农产品加工, 2017(10):4-6;17.
LIAO K M, BAI T H, CHEN C H, et al.Research on the effect of cholesterol lowering in vitro of alpinia officinarum hance crude polysaccharide[J].Farm Products Processing, 2017(10):4-6;17.
[32] DEBRUYNE P R, BRUYNEEL E A, LI X D, et al.The role of bile acids in carcinogenesis[J].Mutation Research/Fundamental & Molecular Mechanisms of Mutagenesis, 2001, 480-481:359-369.
Options
文章导航

/

〈 〉

技术支持：北京玛格泰克科技发展有限公司