为解析重庆地区鲊广椒微生物菌群结构特点,结合高通量测序技术与分离鉴定方法,对重庆地区巫山与奉节采集的鲊广椒菌群进行了分析。结果发现,这两个地区的鲊广椒的优势属有11个,其中乳杆菌属(Lactobacillus),促生乳杆菌属(Levilactobacillus),迟缓乳杆菌属(Lentilactobacillus),伴生乳杆菌属(Companilactobacillus),魏斯氏菌属(Weissella)和普雷沃菌属(Prevotella)含量大于5%。值得注意的是,从巫山鲊广椒检测到了丰度较高的普雷沃菌属和梭菌属(Clostridium)。基于线性判别分析效应值(linear discriminant analysis effect size,LEfSe),普雷沃菌属和塔特姆菌属(Tatumella)分别作为巫山和奉节鲊广椒的生物标志属。通过分离鉴定,从重庆地区鲊广椒中分离到了18株乳酸菌,鉴定为8个菌属。结合高通量测序技术与分离鉴定方法,可将重庆地区鲊广椒的优势属促生乳杆菌属和迟缓乳杆菌属分别鉴定为短促生乳杆菌(Levilactobacillus brevis)和布氏迟缓乳杆菌(Lentilactobacillus buchneri)。该研究预期能为鲊广椒的产业化发展提供菌株资源与数据支撑。
To analyze the characteristics of microbial flora of Zha-chili from Wushan and Fengjie County in Chongqing, high-throughput sequencing and isolation and identification-based methods were used. Based on the results of high-throughput sequencing, there were 11 dominant genera in Zha-chili from the two regions, including Lactobacillus, Levactobacter, Levilactobacillus, Companilactobacter, Weissella, and Prevotella, with the relative abundance of more than 5%. It is notable that there was a high relative abundance of Prevotella and Clostridium in Zha-chili samples from Wushan County. Based on linear discriminant analysis effect size (LEfSe) analysis, Prevotella, and Tatumella could be used as biomarker genera to distinguish Zha-chili in Wushan and Fengjie, respectively. Eighteen LAB strains were isolated from Zha-chili in Chongqing by isolation and identification-based methods, and they were identified as eight genera. Combining high-throughput sequencing and isolation and identification methods, the dominant genera of Levilactobacillus and Lentilactobacillus were identified as Levilactobacillus brevis and Lentilactobacillus buchneri, respectively. This research is expected to provide strain resources and data support for the industrialization development of Zha-chili.
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