食品与发酵工业 >

2023 , Vol. 49 >Issue 24: 235 - 240

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.036809

分析与检测

常见肉制品动物源性的环介导等温扩增高分辨熔解鉴别检测方法

  • 代欣 ,
  • 张月绒 ,
  • 澹台玮 ,
  • 李艳妮 ,
  • 徐秦峰
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  • (陕西科技大学 食品科学与工程学院,陕西 西安,710021)
第一作者:硕士研究生(徐秦峰教授为通信作者,E-mail:xuqinfeng@sust.edu.cn)

收稿日期: 2023-07-18

  修回日期: 2023-09-04

  网络出版日期: 2024-01-17

基金资助

国家自然科学基金项目(22074085)

收起

Identification of animal-derived ingredients in common meat products by integration of loop-mediated isothermal amplification with high-resolution melting

  • DAI Xin ,
  • ZHANG Yuerong ,
  • TANTAI Wei ,
  • LI Yanni ,
  • XU Qinfeng
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  • (School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi′an 710021, China)

Received date: 2023-07-18

  Revised date: 2023-09-04

  Online published: 2024-01-17

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摘要

动物源性成分鉴别在肉制品掺假检测中具有重要意义。为检测肉及其制品中猪、牛、羊3种动物源性成分,该研究建立基于通用引物的环介导等温扩增(loop-mediated isothermal amplification, LAMP)高分辨熔解曲线(high-resolution melting,HRM)检测方法。该方法对猪、牛、羊DNA的检测灵敏度分别可达0.3 pg/μL、0.03 pg/μL、0.3 pg/μL;通过分析通用引物扩增子的熔解曲线可实现牛肉、羊肉中分别掺入5%和1%猪肉的鉴别,并成功应用于市售样品检测。该方法快速、灵敏、准确度高,可以实现仅用1组LAMP引物区分猪、牛、羊3种动物源性成分,是一种有效的动物源性成分鉴别方法,在肉及肉制品动物源性成分检验的快速检测中具有良好的发展前景。

关键词： 肉制品掺假; 通用引物; 环介导等温扩增; 高分辨熔解

本文引用格式

代欣 , 张月绒 , 澹台玮 , 李艳妮 , 徐秦峰 . 常见肉制品动物源性的环介导等温扩增高分辨熔解鉴别检测方法[J]. 食品与发酵工业, 2023 , 49(24) : 235 -240 . DOI: 10.13995/j.cnki.11-1802/ts.036809

Abstract

With the continuous improvement of people′s living standards, the proportion of consumption of meat products has steadily increased, and the frequent occurrence of adulteration issues not only harms the economic interests and health of consumers but also adversely affects meat processing enterprises. Therefore, the identification of animal-derived ingredients is of great significance in the detection of adulteration in meat products. To detect three animal-derived components in meat and its products, a high-resolution melting curve (HRM) detection method based on universal primers was established for LAMP. The sensitivity of this method for detecting DNA from pigs, cows, and sheep could reach 0.3 pg/μL, 0.03 pg/μL, and 0.3 pg/μL. By analyzing the melting curve of the universal primer amplicon, the identification of beef and mutton mixed with 5% and 1% of pork could be achieved, and it was successfully applied to the detection of commercially available samples. This method is fast, sensitive, and highly accurate, and can distinguish three animal-derived ingredients from pigs, cows, and sheep using one set of LAMP primers. It is an effective method for identifying animal-derived components and has good development prospects in the rapid detection of animal-derived components in meat and meat products.

Key words： adulteration of meat products; universal primers; loop-mediated isothermal amplification; high-resolution melting

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