This work was aim to investigate the bactericidal effect of low concentration slightly acidic electrolyzed water (LcSAEW) against Vibrio parahaemolyticus and Shewanella putrefaciens in pure culture and shrimp. In this study, the effects of LcSAEW on the antibacterial effect, cell membrane integrity and cell morphology of both bacteria were analyzed by plate counts, growth curves, conductivity, membrane potential, leakage of intracellular material, reactive oxygen species (ROS) and microscopic observation. The results showed that when pure cultures of V. parahaemolyticus and S. putrefaciens were treated with LcSAEW at available chlorine concentrations of 4 or 8 mg/L for 30 s, the colony count decreased by more than 8 lgCFU/mL. Treatment of shrimp inoculated with two target bacteria with 10 mg/L LcSAEW for 10 min resulted in a reduction in colony counts of 1.6 and 1.25 lgCFU/mL, respectively. The metabolic activity and intracellular ATP concentration were observed decreased in both type of bacteria, whereas, the conductivity, extracellular DNA, protein and K+ contents increased by LcSAEW treatment. In addition, the membrane potential showed hyperpolarization, the intracellular ROS accumulated, and the cell morphology appeared depression and shrinkage. In summary, LcSAEW can destroy cell structure, cell membrane permeability, and therefore, result in disturbance of bacterial activity, and even apoptosis occurs. This study provides a theoretical basis for the application of LcSAEW in aquatic products.
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