为减少水产品资源流失,提高鳖类产品附加值,该文以中华鳖粉为原料,分别采用柠檬酸、胃蛋白酶、酸性蛋白酶、风味蛋白酶、木瓜蛋白酶等方法提取酸溶性胶原和酶溶性胶原,探究鳖源胶原蛋白原料的应用价值。使用氨基酸自动分析仪、扫描电子显微镜、SDS-PAGE凝胶电泳、傅里叶红外光谱仪、气相-质谱联用仪、差式量热扫描仪对胶原蛋白的氨基酸、二级结构、挥发性物质、热稳定性等理化指标进行测定。结果表明,5种胶原蛋白均具有I型胶原特征,且纯度较高,但不同方法间提取效率存在差异。其中,胃蛋白酶溶性胶原蛋白具有较高的提取率与热稳定性,具有亚氨基酸含量高、蛋白交联程度好、气味物质少等优势,在食品与医药领域具有较好的应用前景,为后续鳖源胶原的提取与应用提供了理论基础。
To improve the added value of turtle products and develop new collagen raw materials, acid-soluble collagen (ASC) and enzyme-soluble collagen (ESC) were extracted from Chinese turtle powder by using citric acid, pepsin, acid protease, flavourzyme, and papain respectively. The amino acids, secondary structure, volatile substances, thermal stability, and other physicochemical parameters of the five collagens were determined. Results showed that all five collagens had the characteristics of type I collagen and were of high purity, but there were differences in the extraction efficiency between different methods. Among them, the pepsin-soluble collagen had a higher extraction rate and thermal stability and had the advantages of high subamino acid content, good protein cross-linking, and less odorous substances, which had good application prospects in the food and pharmaceutical fields. This study also provides a theoretical basis for the extraction and application of turtle-derived collagen.
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