食品与发酵工业 >

2024 , Vol. 50 >Issue 4: 286 - 293

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.035542

分析与检测

基于普鲁士蓝纳米粒子的免疫层析法检测小麦中的呕吐毒素

  • 章铜 ,
  • 沈央红 ,
  • 张雯 ,
  • 朱军莉 ,
  • 陆海霞 ,
  • 刘兴泉
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  • 1(浙江工商大学 食品与生物工程学院,浙江省食品安全重点实验室,浙江 杭州,310018)
    2(浙江农林大学 食品与健康学院,浙江 杭州,311300)
第一作者:硕士研究生(朱军莉教授和陆海霞副教授为共同通信作者,E-mail:junlizhu0305@163.com;luhaixia77@zjgsu.edu.cn)

收稿日期: 2023-03-20

  修回日期: 2023-05-19

  网络出版日期: 2024-03-15

基金资助

省“三农九方”科技计划协作项目(2022SNJF011);省尖兵领雁研发攻关计划项目(2023C02026)

收起

Based on Prussian blue nanoparticles for immunochromatographic detection of deoxynivalenol in wheat

  • ZHANG Tong ,
  • SHEN Yanghong ,
  • ZHANG Wen ,
  • ZHU Junli ,
  • LU Haixia ,
  • LIU Xingquan
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  • 1(School of Food Science & Biotechnology, Zhejiang Gongshang University, Zhejiang Key Laboratory of Food Safety, Hangzhou 310018, China)
    2(School of Food and Health, Zhejiang A&F University, Hangzhou 311300, China)

Received date: 2023-03-20

  Revised date: 2023-05-19

  Online published: 2024-03-15

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摘要

该研究采用普鲁士蓝纳米粒子(Prussian blue nanoparticles,PBNPs)作为信号标签,通过制备PBNPs和聚多巴胺包裹普鲁士蓝纳米粒子(polydopamine coated Prussian blue nanoparticles,PB@PDA),优化测试参数、测试试纸条灵敏度和特异性等,研究PBNPs和PB@PDA对呕吐毒素(deoxynivalenol,DON)的检测性能。结果表明,在最优实验条件下,所构建的基于PBNPs和PB@PDA的免疫层析试纸条对DON标准溶液视觉检出限分别为1.0 ng/mL和 0.2 ng/mL,在0.1~0.5 ng/mL保持良好的线性关系,PB@PDA比PBNPs-LFIA检测灵敏度提高5倍,2种试纸条均显示良好的特异性。将PBNPs和PB@PDA两种试纸条应用于小麦样品检测,显示试纸条能排除小麦基质干扰,其检测限分别为20 ng/g和5 ng/g,且PB@PDA检测灵敏度高于商品化胶体金试纸条(10 ng/g)。可见,PB@PDA试纸条表现高灵敏性和抗干扰性,可满足呕吐毒素国家安全标准的限量检测要求,为现场快速筛查小麦中呕吐毒素污染提供一种新方法。

关键词： 普鲁士蓝纳米粒子; 聚多巴胺包裹普鲁士蓝纳米粒子; 免疫层析法; 呕吐毒素; 小麦

本文引用格式

章铜 , 沈央红 , 张雯 , 朱军莉 , 陆海霞 , 刘兴泉 . 基于普鲁士蓝纳米粒子的免疫层析法检测小麦中的呕吐毒素[J]. 食品与发酵工业, 2024 , 50(4) : 286 -293 . DOI: 10.13995/j.cnki.11-1802/ts.035542

Abstract

Mycotoxin contamination such as deoxynivalenol (DON) in cereals and feed is still serious, and rapid detection of DON is a prerequisite for effective control. In this study, Prussian blue nanoparticles (PBNPs) were used as signal tags in the immunochromatographic detection of DON. PBNPs and polydopamine-coated PBNPs (PB@PDA) were prepared for the sensitive detection of DON in wheat. The test parameters, sensitivity and specificity of test strips were optimized. The results showed that under the optimal experimental conditions, the visual detection limits of DON standard solution based on PBNPs and PB@PDA were 1.0 ng/mL and 0.2 ng/mL, respectively, and maintained a good linear relationship between 0.1 and 0.5 ng/mL. Compared with PBNPs, the sensitivity of PB@PDA increased by 5 times, and both test strips showed good specificity. PBNPs and PB@PDA strips were applied in the detection of DON in wheat samples, with detection limits of 1 ng/g and 0.3 ng/g, respectively. The sensitivity of PB@PDA was also higher than that of commercial colloidal gold strips. Thus, these results indicated that PB@PDA strips with high sensitivity and strong tolerance could reach the limit requirements of national safety standards, which would provide a new method for rapid screening of DON on site.

Key words： Prussian blue nanoparticles; polydopamine coated Prussian blue nanoparticles; immunochromatography; deoxynivalenol; wheat

参考文献

[1] 马宏伟, 刘永智.小麦中呕吐毒素检测方法的研究[J].食品安全导刊, 2021(27):73-74.
MA H W, LIU Y Z.Study on detection method of vomiting toxin in wheat[J].China Food Safety Magazine, 2021(27):73-74.
[2] PESTKA J J.Deoxynivalenol:Mechanisms of action, human exposure, and toxicological relevance[J].Archives of Toxicology, 2010, 84(9):663-679.
[3] 朱海华, 张梦雪, 胡骁飞, 等.食品中呕吐毒素检测方法的研究进展[J].食品科技, 2021, 46(11):314-320.
ZHU H H, ZHANG M X, HU X F, et al.Research progress on the detection method of vomitoxin in food[J].Food Science and Technology, 2021, 46(11):314-320.
[4] 贾卫昌, 朱寅.高效液相色谱法与酶联免疫法检测小麦中呕吐毒素的比较研究[J].粮食与食品工业, 2015, 22(3):97-100.
JIA W C, ZHU Y.A comparative research on the determination of deoxynivalenol in wheat with HPLC method and ELISA[J].Cereal and Food Industry, 2015, 22(3):97-100.
[5] 张正炜, 成玮, 沈慧梅, 等.小麦粉中脱氧雪腐镰刀菌烯醇(DON)毒素的气相色谱法检测及毒素污染去除分析[J].农药科学与管理, 2018, 39(4):33-39.
ZHANG Z W, CHENG W, SHEN H M, et al.Determination of deoxynivalenol (DON) toxin in wheat flour by gas chromatography and analysis of toxin pollution removal[J].Pesticide Science and Administration, 2018, 39(4):33-39.
[6] 龚蕾, 周陶鸿, 彭青枝, 等.免疫层析法快速检测动物源性食品中五氯酚酸钠含量[J].食品安全质量检测学报, 2022, 13(3):888-893.
GONG L, ZHOU T H, PENG Q Z, et al.Rapid determination of sodium pentachlorophenolate in animal-derived foods by immunochromatography[J].Journal of Food Safety and Quality, 2022, 13(3):888-893.
[7] GENG X Y, ZHANG F Q, GUO Z H, et al.UV-light-assisted synthesis of CeB6@Ag nano-trees for SERS application[J].Journal of Rare Earths, 2023, 41(1):149-156.
[8] 于绍楠, 任玲玲, 任立群, 等.基于上转换纳米粒子-金纳米棒的荧光共振能量转移免疫分析法用于癌胚抗原检测[J].分析化学, 2022, 50(9):1299-1307.
YU S N, REN L L, REN L Q, et al.Upconversion nanoparticles/gold nanorods-based fluorescence resonance energy transfer immunoassay for detection of carcinoembryonic antigen[J].Chinese Journal of Analytical Chemistry, 2022, 50(9):1299-1307.
[9] 莫紫梅, 袁光蔚, 王海波, 等.同位素稀释液相色谱-串联质谱法和量子点荧光免疫层析法快速测定花生油中黄曲霉毒素B1的比较[J].粮食与饲料工业, 2022(3):59-63;67.
MO Z M, YUAN G W, WANG H B, et al.Comparative study on isotope dilution high performance liquid chromatography-tandem mass spectrometry and quantum dot fluorescence immunochromatography for rapid determination of aflatoxin B1 in pearmtoil[J].Cereal and Feed Industry, 2022(3):59-63;67.
[10] 范耀龙, 陈怡怡, 朱军莉, 等.基于群青蓝纳米粒子的免疫层析法快速检测牛奶中的黄曲霉毒素M1[J].食品安全质量检测学报, 2022, 13(10):3221-3227.
FAN Y L, CHEN Y Y, ZHU J L, et al.Rapid detection of aflatoxin M1 in milk by immunochromatographic assay based on ultramarine blue nanoparticles[J].Journal of Food Safety and Quality, 2022, 13(10):3221-3227.
[11] 冯德香, 黄迎春, 张克, 等.金纳米粒子点缀普鲁士蓝-石墨烯多层膜传感界面的构建及在免疫传感器中的应用[J].分析试验室, 2019, 38(7):819-823.
FENG D X, HUANG Y C, ZHANG K, et al.Preparation and application of electrochemical immunosensor using AuNPs dotted (Prussian blue(PB)-a graphene)n muti-layer films as the sensor platform[J].Chinese Journal of Analysis Laboratory, 2019, 38(7):819-823.
[12] CATALA L, MALLAH T.Nanoparticles of prussian blue analogs and related coordination polymers:From information storage to biomedical applications[J].Coordination Chemistry Reviews, 2017, 346:32-61.
[13] 姚瑶, 冒爱荣, 陈亮, 等.基于聚苯胺-普鲁士蓝/普鲁士蓝复合膜的过氧化氢电化学传感器的制备及表征[J].分析试验室, 2021, 40(12):1467-1471.
YAO Y, MAO A R, CHEN L, et al.Preparation and characterization of hydrogen peroxide electrochemical sensor based on polyaniline-Prussian blue / Prussian blue composite films[J].Chinese Journal of Analysis Laboratory, 2021, 40(12):1467-1471.
[14] 张新红, 赵海华, 常飞, 等.普鲁士蓝比色法快速检测扁桃酸的方法[J].化学工程, 2022, 50(6):1-5;11.
ZHANG X H, ZHAO H H, CHANG F, et al.Rapid colorimetric determination of mandelic acid based on Prussian blue[J].Chemical Engineering (China), 2022, 50(6):1-5;11.
[15] ZHAO B X, HUANG Q, DOU L N, et al.Prussian blue nanoparticles based lateral flow assay for high sensitive determination of clenbuterol[J].Sensors and Actuators B: Chemical, 2018, 275:223-229.
[16] LU D, JIANG H, ZHANG G Y, et al.An in situ generated Prussian blue nanoparticle-mediated multimode nanozyme-linked immunosorbent assay for the detection of aflatoxin B1[J].ACS Applied Materials and Interfaces, 2021, 13(22):25 738-25 747.
[17] YE W C, HUANG H, YANG W W, et al.Ultrathin polydopamine film coated gold nanoparticles:A sensitive, uniform, and stable SHINERS substrate for detection of benzotriazole[J].Analyst, 2017, 142(18):3459-3467.
[18] XU S L, ZHANG G G, FANG B L, et al.Lateral flow immunoassay based on polydopamine-coated gold nanoparticles for the sensitive detection of Zearalenone in maize[J].ACS Applied Materials &Interfaces, 2019, 11(34):31283-31290.
[19] 杜艳军, 李小燕, 刘义保, 等.多巴胺包覆纳米普鲁士蓝的制备及其对Sr~(2+)的吸附性能研究[J].有色金属工程, 2021, 11(12):115-121.
DU Y J, LI X Y, LIU Y B, et al.Preparation of dopamine coated nano-prussian blue and study on its adsorption performance for Sr 2+[J].Nonferrous Metals Engineering, 2021,11(12):115-121.
[20] SHAO Y N, DUAN H, GUO L, et al.Quantum dot nanobead-based multiplexed immunochromatographic assay for simultaneous detection of aflatoxin B1 and zearalenone[J].Analytica Chimica Acta, 2018, 1025:163-171.
[21] BU T, BAI F E, SUN X Y, et al.An innovative Prussian blue nanocubes decomposition-assisted signal amplification strategy suitable for competitive lateral flow immunoassay to sensitively detect aflatoxin B1[J].Food Chemistry, 2021, 344:128711.
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