食品与发酵工业 >

2024 , Vol. 50 >Issue 3: 73 - 79

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.033892

研究报告

硫酸软骨素纳米硒的结构表征及其对Hela细胞迁移和侵袭的影响

  • 陈雪花 ,
  • 陈建平 ,
  • 罗宝浈 ,
  • 李佳睿 ,
  • 李瑞 ,
  • 刘晓菲 ,
  • 宋兵兵 ,
  • 钟赛意
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  • 1(广东海洋大学 食品科技学院,广东省水产品加工与安全重点实验室,广东省海洋生物制品工程实验室,广东省海洋食品工程技术研究中心,水产品深加工广东普通高等学校重点实验室,广东省亚热带果蔬加工科技创新中心,广东 湛江,524088)
    2(海洋食品精深加工关键技术省部共建协同创新中心(大连工业大学),辽宁 大连,116034)
第一作者:硕士研究生(陈建平副教授为通信作者,E-mail:cjp516555989@126.com)

收稿日期: 2022-10-09

  修回日期: 2022-11-24

  网络出版日期: 2024-03-15

基金资助

广东省自然科学基金面上项目(2020A1515010860,2021A1515012455);湛江市科技计划项目(2022A01045);广东海洋大学“南海学者计划”项目(002029002009);广东海洋大学创新强校项目(230419100);广东省普通高校创新团队项目(2021KCXTD021);广东海洋大学优秀学位论文培育项目(040505042203)

收起

Structural characterization of selenium-chondroitin sulfate nanoparticles and its effect on migration and invasion of Hela cells

  • CHEN Xuehua ,
  • CHEN Jianping ,
  • LUO Baozhen ,
  • LI Jiarui ,
  • LI Rui ,
  • LIU Xiaofei ,
  • SONG Bingbing ,
  • ZHONG Saiyi
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  • 1(College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Provincial Engineering Laboratory for Marine Biological Products, Guangdong Province Engineering, Technology Research Center of Seafood, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, Guangdong Provincial Modern Agricultural Science and Technology Innovation Center for Subtropical Fruit and Vegetable Processing, Zhanjiang 524088, China)
    2(Collaborative Innovation Center of Seafood Deep Processing (Dalian Polytechnic University), Dalian 116034, China)

Received date: 2022-10-09

  Revised date: 2022-11-24

  Online published: 2024-03-15

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摘要

对硫酸软骨素纳米硒(selenium-chondroitin sulfate nanoparticles, SeCS)的结构进行鉴定,并考察其对Hela细胞迁移和侵袭的影响。以硫酸软骨素为模板,采用亚硒酸钠-维生素C氧化还原法制备SeCS,并运用透射电镜、扫描电镜、X射线光电子能谱仪和傅立叶红外光谱仪对SeCS进行结构表征;通过划痕实验和细胞迁移侵袭实验(Transwell)检测SeCS对Hela细胞迁移及侵袭的影响;通过Western blot免疫印迹法检测SeCS对细胞内基质金属蛋白酶-2(matrix metalloproteinase 2, MMP-2)和基质金属蛋白酶-9(matrix metalloproteinase 9, MMP-9)表达水平的影响。实验结果表明,制备所得的SeCS是零价态的分散良好的球形纳米粒,表面光滑。Hela细胞经20 μg/mL SeCS处理后,细胞的迁移率和侵袭率分别从对照组的100%下降到(59.19±7.74)%和(82.43±4.21)%,表明SeCS能够抑制细胞的迁移和侵袭。进一步的研究发现,SeCS下调了MMP-2和MMP-9蛋白的表达量,表明SeCS通过降低MMP-2和MMP-9的蛋白表达来抑制细胞的迁移和侵袭。

关键词： 硫酸软骨素纳米硒; 结构表征; Hela细胞; 迁移; 侵袭

本文引用格式

陈雪花 , 陈建平 , 罗宝浈 , 李佳睿 , 李瑞 , 刘晓菲 , 宋兵兵 , 钟赛意 . 硫酸软骨素纳米硒的结构表征及其对Hela细胞迁移和侵袭的影响[J]. 食品与发酵工业, 2024 , 50(3) : 73 -79 . DOI: 10.13995/j.cnki.11-1802/ts.033892

Abstract

This study aimed to identify the structure of selenium-chondroitin sulfate nanoparticles (SeCS) and investigate its effect on the migration and invasion of Hela cells. SeCS were prepared by the sodium selenite (Na2SeO3) and ascorbic acid redox reaction using chondroitin sulfate as a template and characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FTIR). The effects of SeCS on the migration and invasion of Hela cells were detected by scratch test and cell migration and invasion assay (Transwell). The effects of SeCS on matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) expression levels were detected using western blot. Results showed that the prepared SeCS were zero-valent spherical nanoparticles with a smooth surface. After Hela cells were treated with 20 μg/mL SeCS, the migration and invasion rates of cells were decreased from 100% in the control group to (59.19±7.74)% and (82.43±4.21)%, respectively, indicating that SeCS could inhibit cell migration and invasion. Further studies found that SeCS down-regulated the expression levels of MMP-2 and MMP-9 proteins, indicating that SeCS inhibited cell migration and invasion by reducing the expression of MMP-2 and MMP-9 proteins.

Key words： selenium-chondroitin sulfate nanoparticles; structural characterization; Hela cells; migration; invasion

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