该文使用高压均质手段构建脂质体以提高辅酶Q10(coenzyme Q10,CoQ10)的稳定性和生物可接受率。采用乙醇注入法结合高压均质技术制备脂质体,并分析其粒径、多分散指数、电位和包封率等理化指标,评价CoQ10脂质体的储藏稳定性和模拟消化特性。结果表明,所制备的脂质体为球形,包封率均在96%。50、100 MPa的高压均质处理将脂质体的粒径从(538.26±11.25) nm分别降低为(240.21±7.23)、(130.17±3.13) nm,而对脂质体的电位和包封率无显著影响。同时,高压均质处理有效延缓了储藏期间CoQ10脂质体酸价、过氧化值的增长,但过高的均质压力对脂质体稳定性具有不利影响。模拟体外消化显示,脂质体明显提高了CoQ10的生物可接受率,高压均质处理将该值分别提高至(31.97±1.62)%和(31.61±2.72)%。实验结果表明,采用高压均质技术制备脂质体是实现CoQ10高稳定和高生物可接受率递送的可靠方法,对CoQ10相关产品的开发具有参考价值和指导意义。
High pressure homogenization was used to construct liposomes to improve the stability and bioaccessibility of coenzyme Q10 (CoQ10). Liposomes were prepared by ethanol injection method combined with high pressure homogenization technology, and their physicochemical indexes such as particle size, polydispersity index, potential, and encapsulation efficiency were analyzed, and the storage stability and simulated digestion characteristics of CoQ10 liposomes were evaluated. The result revealed that the prepared liposomes were spherical with the encapsulation efficiency of about 96%. The high pressure homogenization at 50 MPa and 100 MPa reduced the particle size of liposomes from (538.26±11.25) nm to (240.21±7.23) nm and (130.17±3.13) nm, respectively, without significant effect on the potential and encapsulation efficiency of liposomes. At the same time, high pressure homogenization treatment effectively delayed the increase of acid value and peroxide value of CoQ10 liposomes during storage, but too high homogenization pressure had an adverse effect on liposome stability. In vitro simulated digestion experiments showed that liposomes significantly increased the bioaccessibility of CoQ10, and high pressure homogenization increased the values to (31.97±1.62)% and (31.61±2.72)%, respectively. The experimental results show that the preparation of liposomes by high pressure homogenization technology is a reliable method to achieve high stability and high bioaccessibility delivery of CoQ10, which has reference value and guiding significance for the development of CoQ10-related products.
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