食品与发酵工业 >

2024 , Vol. 50 >Issue 6: 130 - 137

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.034542

研究报告

百香果提取液绿色制备银纳米粒子及其抗菌研究

  • 曹兴业 ,
  • 谢闰生 ,
  • 赵志远 ,
  • 李培骏 ,
  • 李海云
展开
  • 1(桂林理工大学 化学与生物工程学院,广西 桂林,541004)
    2(韶关学院 广东省粤北食药资源利用与保护重点实验室,广东 韶关,512005)
第一作者:硕士研究生(李培骏副教授和李海云教授为共同通信作者,E-mail:peijunli@sgu.edu.cn;lihaiyun@glut.edu.cn)

收稿日期: 2022-12-06

  修回日期: 2023-02-20

  网络出版日期: 2024-04-17

基金资助

广西自然科学基金项目(2020GXNSFAA297198);广西科技基地和人才专项(桂科AD19110074)

收起

Green preparation of silver nanoparticles from passion fruit extract and their antibacterial research

  • CAO Xingye ,
  • XIE Runsheng ,
  • ZHAO Zhiyuan ,
  • LI Peijun ,
  • LI Haiyun
Expand
  • 1(College of Chemistry & Bioengineering, Guilin University of Technology, Guilin 541004, China)
    2(Provincial Key Laboratory for Utilization and Conservation of Food and Medicinal Resources in Northern Guangdong, Shaoguan University, Shaoguan 512005, China)

Received date: 2022-12-06

  Revised date: 2023-02-20

  Online published: 2024-04-17

Fold

摘要

目前,人们已经提出了很多用于合成银纳米粒子的化学方法,其中绿色合成银纳米粒子的方法由于其低成本和环境友好性而受到更多关注。该研究报道了以百香果果皮提取物为原料绿色合成银纳米粒子的方法并研究其抗菌性能。通过紫外可见光谱在420 nm处出现峰值来确定银纳米颗粒的形成,以透射电子显微镜和扫描电子显微镜表征其粒径大小及表面形态,纳米银(AgNPs)多呈球形,分散性良好,平均粒径为12.1 nm;采用X射线衍射分析AgNPs结晶性质并证明了AgNPs的存在;使用光电子能谱仪分析Ag价态为0价;热重分析仪测定结果表明了AgNPs有良好的热稳定性;傅里叶红外光谱及拉曼散射光谱测试结果显示,百香果果皮提取物具有还原和稳定银纳米粒子的双重功能,其中具有该作用的活性物质可能是蛋白质、多酚、黄酮和果胶等。大肠杆菌和金黄色葡萄球菌最小抑制质量浓度分别为33、66 μg/mL。综上所述,利用百香果提取液可以绿色制备较为理想的AgNPs,有望在制药、工业等抑菌领域得到广泛应用。

关键词： 绿色合成; 纳米银; 百香果; 抗菌; 表征

本文引用格式

曹兴业 , 谢闰生 , 赵志远 , 李培骏 , 李海云 . 百香果提取液绿色制备银纳米粒子及其抗菌研究[J]. 食品与发酵工业, 2024 , 50(6) : 130 -137 . DOI: 10.13995/j.cnki.11-1802/ts.034542

Abstract

In this study, silver nanoparticles (AgNPs) were synthesized from passion fruit peel extract and their antimicrobial properties were studied.The formation of AgNPs was determined by a UV-Vis peak at 420 nm.Transmission electron microscope (TEM) and scanning electron microscope (SEM) were used to characterize the particle size and surface morphology of AgNPs.Most of the AgNPs were spherical with good dispersion and the average particle size was 12.1 nm.X-ray diffraction (XRD) analysis of AgNPs analyzed the crystallization properties of AgNPs and demonstrated the presence of AgNPs.The thermogravimetric analyser (TGA) indicated good thermal stability of the AgNPs.Fourier transform infrared spectroscopy (FTIR) and Raman scattering spectroscopy results showed that the passion fruit peel extract had the dual functions of reducing and stabilizing silver nanoparticles, among which the active substances might be protein, polyphenols, flavonoids, and pectin.The minimum inhibitory concentration of Escherichia coli and Staphylococcus aureus were 33.3 μg/mL and 66.7 μg/mL, respectively.In conclusion, the ideal nano-silver can be prepared green by using passion fruit extract, which is expected to be widely used in antibacterial fields such as pharmacy and industry.

Key words： green synthesis; silver nanoparticles; passion fruit; antibacterial; characterization

参考文献

[1] AHMED S, AHMAD M, SWAMI B L, et al.A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications:A green expertise[J].Journal of Advanced Research, 2016, 7 (1):17-28.
[2] AMEEN F, SRINIVASAN P, SELVANKUMAR T, et al.Phytosynthesis of silver nanoparticles using Mangifera indica flower extract as bioreductant and their broad-spectrum antibacterial activity[J].Bioorganic Chemistry, 2019, 88:102970.
[3] GOVARTHANAN M, CHO M, PARK J H, et al.Cottonseed oilcake extract mediated green synthesis of silver nanoparticles and its antibacterial and cytotoxic activity[J].Journal of Nanomaterials, 2016, 2016:7412431.
[4] LEE K J, PARK S H, GOVARTHANAN M, et al.Synthesis of silver nanoparticles using cow milk and their antifungal activity against phytopathogens[J].Materials Letters, 2013, 105:128-131.
[5] FOUDA M M G, ABDELSALAM N R, GOHAR I M A, et al.Utilization of High throughput microcrystalline cellulose decorated silver nanoparticles as an eco-nematicide on root-knot nematodes[J].Colloids and Surfaces B: Biointerfaces, 2020, 188:110805.
[6] VALARMATHI N, AMEEN F, ALMANSOB A, et al.Utilization of marine seaweed Spyridia filamentosa for silver nanoparticles synthesis and its clinical applications[J].Materials Letters, 2020, 263:127244.
[7] EL-NAGGAR M E, SHAARAWY S, HEBEISH A A.Bactericidal finishing of loomstate, scoured and bleached cotton fibres via sustainable in-situ synthesis of silver nanoparticles[J].International Journal of Biological Macromolecules, 2018, 106:1192-1202.
[8] CHOI M S, NA H G, SHIM G S, et al.Simple and scalable synthesis of urchin-like ZnO nanoparticles via a microwave-assisted drying process[J].Ceramics International, 2021, 47(10):14621-14629.
[9] ZHOU C H, ZHANG Y, YAN F H, et al.Vapor deposition synthesis of polypyrrole nanoparticles with a tunable photothermal conversion capacity[J].Colloids and Surfaces A:Physicochemical and Engineering Aspects, 2021, 613:126073.
[10] ALI EBRAHIMZADEH M, MORTAZAVI-DERAZKOLA S, ALI ZAZOULI M.Eco-friendly green synthesis of novel magnetic Fe3O4/SiO2/ZnO-Pr6O11 nanocomposites for photocatalytic degradation of organic pollutant[J].Journal of Rare Earths, 2020, 38(1):13-20.
[11] MALATHI S, EZHILARASU T, ABIRAMAN T, et al.One pot green synthesis of Ag, Au and Au-Ag alloy nanoparticles using isonicotinic acid hydrazide and starch[J].Carbohydrate Polymers, 2014, 111:734-743.
[12] LALIWALA S K, MEHTA V N, ROHIT J V, et al.Citrate-modified silver nanoparticles as a colorimetric probe for simultaneous detection of four triptan-family drugs[J].Sensors and Actuators B:Chemical, 2014, 197:254-263.
[13] MUTHUSAMY G, THANGASAMY S, RAJA M, et al.Biosynthesis of silver nanoparticles from Spirulina microalgae and its antibacterial activity[J].Environmental Science and Pollution Research International, 2017, 24(23):19459-19464.
[14] ABDELGAWAD A M, EL-NAGGAR M E, EISA W H, et al.Clean and high-throughput production of silver nanoparticles mediated by soy protein via solid state synthesis[J].Journal of Cleaner Production, 2017, 144:501-510.
[15] GOVARTHANAN M, SEO Y S, LEE K J, et al.Low-cost and eco-friendly synthesis of silver nanoparticles using coconut (Cocos nucifera) oil cake extract and its antibacterial activity[J].Artificial Cells, Nanomedicine, and Biotechnology, 2016, 44(8):1878-1882.
[16] MYTHILI R, SELVANKUMAR T, KAMALA-KANNAN S, et al.Utilization of market vegetable waste for silver nanoparticle synthesis and its antibacterial activity[J].Materials Letters, 2018, 225:101-104.
[17] CAO Q Q, TENG J W, WEI B Y, et al.Phenolic compounds, bioactivity, and bioaccessibility of ethanol extracts from passion fruit peel based on simulated gastrointestinal digestion[J].Food Chemistry, 2021, 356:129682.
[18] DE ARAÚJO F F, DE PAULO FARIAS D, NERI-NUMA I A, et al.Polyphenols and their applications:An approach in food chemistry and innovation potential[J].Food Chemistry, 2021, 338:127535.
[19] SU D L, LI P J, NING M, et al.Microwave assisted green synthesis of pectin based silver nanoparticles and their antibacterial and antifungal activities[J].Materials Letters, 2019, 244:35-38.
[20] LI P J, LIANG J Y, SU D L, et al.Green and efficient biosynthesis of pectin-based copper nanoparticles and their antimicrobial activities[J].Bioprocess and Biosystems Engineering, 2020, 43(11):2017-2026.
[21] DONG C F, ZHANG X L, CAI H.Green synthesis of monodisperse silver nanoparticles using hydroxy propyl methyl cellulose[J].Journal of Alloys and Compounds, 2014, 583:267-271.
[22] ZHA J L, DONG C F, WANG X J, et al.Green synthesis and characterization of monodisperse gold nanoparticles using Ginkgo Biloba leaf extract[J].Optik, 2017, 144:511-521.
[23] FATIMAH I, AFTRID Z H V I.Characteristics and antibacterial activity of green synthesized silver nanoparticles using red spinach (Amaranthus Tricolor L.) leaf extract[J].Green Chemistry Letters and Reviews, 2019, 12(1):25-30.
[24] BALCIUNAITIENE A,VISKELIS P,VISKELIS J, et al.Green synthesis of silver nanoparticles using extract of Artemisia absinthium L. Humulus lupulus L. and Thymus vulgaris L. physico-chemical characterization, antimicrobial and antioxidant Activity[J].Processes, 2021, 9(8):1304.
[25] KHAN T, ALI G S.Variation in surface properties, metabolic capping, and antibacterial activity of biosynthesized silver nanoparticles:Comparison of bio-fabrication potential in phytohormone-regulated cell cultures and naturally grown plants[J].RSC Advances, 2020, 10(64):38831-38840.
[26] EDISON T I, SETHURAMAN M G.Biogenic robust synthesis of silver nanoparticles using Punica granatum peel and its application as a green catalyst for the reduction of an anthropogenic pollutant 4-nitrophenol[J].Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2013, 104:262-264.
[27] ZHANG Y, JIANG J J, LI M, et al.Green synthesis of gold nanoparticles with pectinase:A highly selective and ultra-sensitive colorimetric assay for Mg2+[J].Plasmonics, 2017, 12(3):717-727.
[28] SINGH S, SAIKIA J P, BURAGOHAIN A K.A novel reusable PAni-PVA-Amylase film:Activity and analysis[J].Colloids and Surfaces.B,Biointerfaces, 2013, 106:46-50.
[29] SONG Z Y, WU Y, WANG H J, et al.Synergistic antibacterial effects of curcumin modified silver nanoparticles through ROS-mediated pathways[J].Materials Science & Engineering.C,Materials for Biological Applications, 2019, 99:255-263.
[30] DENDISOVÁ-VYSKOVSKÁ M, KOKAISLOVÁ A, ONĆÁK M, et al.SERS and in situ SERS spectroscopy of riboflavin adsorbed on silver, gold and copper substrates.Elucidation of variability of surface orientation based on both experimental and theoretical approach[J].Journal of Molecular Structure, 2013, 1038:19-28.
[31] GOVARTHANAN M, SELVANKUMAR T, MANOHARAN K, et al.Biosynthesis and characterization of silver nanoparticles using panchakavya, an Indian traditional farming formulating agent[J].International Journal of Nanomedicine, 2014, 9:1593-1599.
[32] BECHERI A, DÜRR M, LONOSTRO P, et al.Synthesis and characterization of zinc oxide nanoparticles:Application to textiles as UV-absorbers[J].Journal of Nanoparticle Research, 2008, 10(4):679-689.
[33] HAGLAN A M, ABBAS H S, AKKÖZ C, et al.Characterization and antibacterial efficiency of silver nanoparticles biosynthesized by using green algae Enteromorpha intestinalis[J].International Nano Letters, 2020, 10(3):197-205.
[34] FERRARIS S, MIOLA M, COCHIS A, et al.In situ reduction of antibacterial silver ions to metallic silver nanoparticles on bioactive glasses functionalized with polyphenols[J].Applied Surface Science, 2017, 396:461-470.
[35] PRIETO P, NISTOR V, NOUNEH K,et al.XPS study of silver, nickel and bimetallic silver-nickel nanoparticles prepared by seed-mediated growth[J].Applied Surface Science, 2012, 258(22):8807-8813.
[36] BUSZEWSKI B, RAILEAN-PLUGARU V, POMASTOWSKI P, et al.Antimicrobial activity of biosilver nanoparticles produced by a novel Streptacidiphilus durhamensis strain[J].Journal of Microbiology Immunology and Infection, 2018, 51(1):45-54.
[37] HU G S, JIN W X, CHEN Q Y, et al.Antibacterial activity of silver nanoparticles with different morphologies as well as their possible antibacterial mechanism[J].Applied Physics A, 2016, 122(10):874.
[38] CHINNAPPAN S, KANDASAMY S, ARUMUGAM S, et al.Biomimetic synthesis of silver nanoparticles using flower extract of Bauhinia purpurea and its antibacterial activity against clinical pathogens[J].Environmental Science and Pollution Research International, 2018, 25(1):963-969.
Options
文章导航

/

〈 〉

技术支持：北京玛格泰克科技发展有限公司