食品与发酵工业 >

2024 , Vol. 50 >Issue 6: 192 - 200

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.034740

研究报告

响应面优化提取海蒿子多酚及其抗氧化和抗糖尿病活性分析

  • 谢星 ,
  • 王乐怀 ,
  • 林文静 ,
  • 刘晓欢 ,
  • 张露 ,
  • 涂宗财
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  • 1(江西师范大学 健康学院,江西 南昌,330022)
    2(南昌大学,食品科学与资源挖掘实验室,江西 南昌,330047)
第一作者:博士,讲师(涂宗财教授为通信作者,E-mail:tuzc_mail@aliyun.com)

收稿日期: 2022-12-28

  修回日期: 2023-01-31

  网络出版日期: 2024-04-17

基金资助

江西省青年科学基金项目(20224BAB215051)

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Optimization of extraction of phenolics from Sargassum pallidum by response surface methodology and its antioxidant and antidiabetic activities

  • XIE Xing ,
  • WANG Lehuai ,
  • LIN Wenjing ,
  • LIU Xiaohuan ,
  • ZHANG Lu ,
  • TU Zongcai
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  • 1(College of Health, Jiangxi Normal University, Nanchang 330022, China)
    2(State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, China)

Received date: 2022-12-28

  Revised date: 2023-01-31

  Online published: 2024-04-17

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摘要

海蒿子是一种可药食两用的褐藻,具有多种功能活性。该研究采用响应面法优化了海蒿子多酚超声辅助提取的工艺,并测定了海蒿子多酚提取物及其萃取组分的总酚含量、抗氧化和抗糖尿病活性。结果表明,响应面优化超声辅助提取的最佳条件为乙醇体积分数8.5%,提取时间55 min,提取温度42 ℃,超声功率494 W,海蒿子多酚的含量为1.81 mg GAE/g DM。海蒿子粗提物正丁醇相的总酚含量最高,为24.45 mg GAE/g E。生物活性研究表明,二氯甲烷相的DPPH自由基清除能力(49.34 μmol TE/g)和ABTS阳离子自由基清除能力(IC50=249.22 μg/mL)最强。乙酸乙酯和二氯甲烷相的α-葡萄糖苷酶抑制活性明显高于阿卡波糖,为其9.61和10.33倍。同时,乙酸乙酯相有最强的DPP-Ⅳ酶抑制活性(IC50=25.83 μg/mL),该溶剂能很好地富集海蒿子中的抗糖尿病成分。相关性分析表明,多酚是海蒿子中主要的抗氧化剂,α-葡萄糖苷酶和DPP-Ⅳ酶抑制活性呈高度相关。该研究发现海蒿子多酚作为多靶点抗糖尿病药物或健康食品的潜力很大,可促进海蒿子高附加值产品的开发。

关键词： 海蒿子; 多酚; 响应面; 抗氧化活性; 抗糖尿病活性

本文引用格式

谢星 , 王乐怀 , 林文静 , 刘晓欢 , 张露 , 涂宗财 . 响应面优化提取海蒿子多酚及其抗氧化和抗糖尿病活性分析[J]. 食品与发酵工业, 2024 , 50(6) : 192 -200 . DOI: 10.13995/j.cnki.11-1802/ts.034740

Abstract

Sargassum pallidum is an edible and medicinal brown seaweed with various functional activities.In this study, the ultrasonic-assisted extraction technology of phenolics from Sargassum pallidum was optimized by using the response surface method.The total phenolics content, antioxidant, and antidiabetic activities of polyphenol extract in Sargassum pallidum and its fraction were also investigated.Results indicated that the optimum extraction process condition was the ethanol concentration of 8.5%, extraction time of 55 min, extraction temperature of 42 ℃, and ultrasonic power of 494 W, the phenolics content of Sargassum pallidum was 1.81 mg GAE/g DM.The n-butanol fraction of crude extract from Sargassum pallidum showed the highest phenolics content with a value of 24.45 mg GAE/g E.Biological studies displayed that dichloromethane fraction gave the strongest DPPH radical scavenging capacity (49.34 μmol TE/g) and ABTS cationic radical scavenging capacity (IC50=249.22 μg/mL).The α-glucosidase inhibitory of dichloromethane and ethyl acetate fractions was 10.33 and 9.61 times higher than that of acarbose.Meanwhile, the ethyl acetate fraction had the best DPP-Ⅳ enzyme inhibition activity with an IC50 value of 25.83 μg/mL, and the solvent showed a good enrichment effect on antidiabetic components in Sargassum pallidum.Correlative analysis suggested that phenolics were the major antioxidants in Sargassum pallidum, and the inhibition activities of α-glucosidase and DPP-Ⅳ enzyme were closely correlated.This study found that the phenolics in Sargassum pallidum showed great potential as multi-target drugs or health food, which also could promote the exploration of high value-added products from Sargassum pallidum.

Key words： Sargassum pallidum; phenolics; response surface methodology; antioxidation activity; antidiabetic activity

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