综述与专题评论

调控质粒拷贝数优化生物催化效率的研究进展

  • 李业 ,
  • 严豪 ,
  • 刘梦婷 ,
  • 白仲虎
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  • 1(江南大学 生物工程学院,江苏 无锡,214122)
    2(江南大学,粮食发酵工艺与技术国家工程实验室,江苏 无锡,214122)
    3(清华大学,深圳国际研究生院生物医药与健康工程研究院,广东 深圳,518000)
第一作者:博士,助理研究员(白仲虎教授为通信作者,E-mail:baizhonghu@jiangnan.edu.cn)

收稿日期: 2023-09-26

  修回日期: 2023-12-04

  网络出版日期: 2024-04-17

基金资助

国家自然科学基金面上项目(21878142);江南大学青年基金(JUSPR12057)

Research progress on plasmid copy number regulation to optimize biocatalytic efficiency

  • LI Ye ,
  • YAN Hao ,
  • LIU Mengting ,
  • BAI Zhonghu
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  • 1(School of Biotechnology, Jiangnan University, Wuxi 214122, China)
    2(National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China)
    3(Institute of Biopharmaceutical and Health Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518000, China)

Received date: 2023-09-26

  Revised date: 2023-12-04

  Online published: 2024-04-17

摘要

质粒作为外源基因异源表达的普遍载体,在合成生物学中广泛应用。不同质粒载体在细胞工厂中的拷贝数不尽相同,合理利用拷贝数不同的质粒可以优化目标基因表达水平,提高生物催化效率。针对天然质粒复制子系统的局限,近年来研究者们依据质粒复制机制,开发出了许多性能更优异的新质粒系统,显著提高了生物催化效率。该文结合质粒拷贝数在多种微生物细胞工厂中的应用进行综述,以期为合成生物学领域的相关研究提供参考和借鉴。

本文引用格式

李业 , 严豪 , 刘梦婷 , 白仲虎 . 调控质粒拷贝数优化生物催化效率的研究进展[J]. 食品与发酵工业, 2024 , 50(6) : 282 -289 . DOI: 10.13995/j.cnki.11-1802/ts.037494

Abstract

As a universal vector for heterologous expression of foreign genes, plasmids are widely used in synthetic biology.Different plasmid vectors have different copy numbers in cell factories.Rational use of plasmids with different copy numbers can optimize the expression level of target genes and improve biocatalytic efficiency.In response to the limitations of natural plasmid replicon systems, in recent years researchers have developed many new plasmid systems with better performance based on plasmid replication and copy number control mechanisms, significantly improving biocatalytic efficiency.This article reviewed plasmid systems in various microbial cell factories along with plasmid copy number engineering and applications in optimizing biocatalytic efficiency, providing a reference for related research in the field of synthetic biology.

参考文献

[1] RODRÍGUEZ-BELTRÁN J, DELAFUENTE J, LEÓN-SAMPEDRO R, et al. Beyond horizontal gene transfer: The role of plasmids in bacterial evolution[J]. Nature Reviews. Microbiology, 2021, 19(6):347-359.
[2] 姚庆智. 细菌质粒及其在基因工程中的应用[J]. 内蒙古科技与经济, 2000(5):24-26.
YAO Q Z. Bacterial plasmid and its application in genetic engineering[J]. Inner Mongolia Science Technolgy and Economy, 2000(5):24-26.
[3] SHETTY R P, ENDY D, JR KNIGHT T F. Engineering BioBrick vectors from BioBrick parts[J]. Journal of Biological Engineering, 2008, 2:5.
[4] 王宇鹏. qPCR方法检测不同条件下重组乳杆菌的pLA质粒拷贝数[D]. 大庆: 黑龙江八一农垦大学, 2014.
WANG Y P. The pLA plasmid of recombinant Lactobacillus qPCR method for detection of different conditions of PCN[D]. Daqing: Heilongjiang Bayi Agricultural University, 2014.
[5] KARL F. Plasmid copy number and plasmid stability[J]. Advances in Biochemical Engineering/Biotechnology, 2004, 86:47-82.
[6] 冯尔玲, 王恒樑, 林云, 等. 质粒拷贝数对宋内氏菌I相O-抗原表达的影响[J]. 生物技术通讯, 2000, 11(3):202-205.
FENG E L, WANG H L, LIN Y, et al. Influence of plasmid copies on the expression of Shigella sonnei form I O-antigen[J]. Letters in Biotechnology, 2000, 11(3):202-205.
[7] 邓明勇, 李画敏, 魏子贡. lys1-d缺陷型标记介导毕赤酵母超高拷贝质粒整合[J]. 湖北大学学报(自然科学版), 2020, 42(5):471-475; 483.
DENG M Y, LI H M, WEI Z G. Superhigh-copy plasmid integration in Komagataella phaffii mediated by lys1-d auxotrophic marker[J]. Journal of Hubei University (Natural Science), 2020, 42(5):471-475; 483.
[8] ROUCHES M V, XU Y S, CORTES L B G, et al. A plasmid system with tunable copy number[J]. Nature Communications, 2022, 13(1):3908.
[9] DEL SOLAR G, GIRALDO R, RUIZ-ECHEVARRÍA M J, et al. Replication and control of circular bacterial plasmids[J]. Microbiology and Molecular Biology Reviews: MMBR, 1998, 62(2):434-464.
[10] 付立霞, 高雯, 韩先干, 等. 实时定量PCR测定爱德华氏菌隐蔽质粒的拷贝数[J]. 淡水渔业, 2018, 48(4):63-70.
FU L X, GAO W, HAN X G, et al. Real time PCR quantification of the copy number of cryptic plasmids in Edwardsiella ictaluri[J]. Freshwater Fisheries, 2018, 48(4):63-70.
[11] SCHMIDT T, FRIEHS K, FLASCHEL E. Rapid determination of plasmid copy number[J]. Journal of Biotechnology, 1996, 49(1-3):219-229.
[12] WATVE M M, DAHANUKAR N, WATVE M G. Sociobiological control of plasmid copy number in bacteria[J]. PLoS One, 2010, 5(2): e9328.
[13] SAN MILLAN A, HEILBRON K, MACLEAN R C. Positive epistasis between co-infecting plasmids promotes plasmid survival in bacterial populations[J]. The ISME Journal, 2014, 8(3):601-612.
[14] LIAN J Z, JIN R, ZHAO H M. Construction of plasmids with tunable copy numbers in Saccharomyces cerevisiae and their applications in pathway optimization and multiplex genome integration[J]. Biotechnology and Bioengineering, 2016, 113(11):2462-2473.
[15] 钟春英. 苏云金芽胞杆菌菌株YBT-1520内生质粒生物量的鉴定[D]. 武汉: 华中农业大学, 2010.
ZHONG C Y. Evaluation of plasmid DNA amount in Bacillus Thuringiensis strain YBT-1520[D]. Wuhan: Huazhong Agricultural University, 2010.
[16] JAHN M, GÜNTHER S, MÜLLER S. Non-random distribution of macromolecules as driving forces for phenotypic variation[J]. Current Opinion in Microbiology, 2015, 25:49-55.
[17] PROVIDENTI M A, O′BRIEN J M, EWING R J, et al. The copy-number of plasmids and other genetic elements can be determined by SYBR-Green-based quantitative real-time PCR[J]. Journal of Microbiological Methods, 2006, 65(3):476-487.
[18] SUMMERS D K. The kinetics of plasmid loss[J]. Trends in Biotechnology, 1991, 9(1):273-278.
[19] 周涛. 质粒拷贝数的测定方法[J]. 生物技术通讯, 1999, 10(1):51-57.
ZHOU T. Determination methods of plasmid copy number[J]. Letters in Biotechnology, 1999, 10(1):51-57.
[20] 王剑, 夏杰, 陆兵, 等. 培养基组分对hCG核酸疫苗质粒拷贝数的影响[J]. 化学与生物工程, 2009, 26(5):46-49.
WANG J, XIA J, LU B, et al. Effect of medium composition on plasmid amplification of hCG DNA vaccine[J]. Chemistry & Bioengineering, 2009, 26(5):46-49.
[21] 丁满生, 马文峰, 郭美锦, 等. 温度诱导模式对重组大肠杆菌质粒拷贝数的影响[J]. 华东理工大学学报(自然科学版), 2006, 32(1):33-37.
DING M S, MA W F, GUO M J, et al. Effects of temperature induction modes on the plasmid copy number in Escherichia coli[J]. Journal of East China University of Science and Technology, 2006, 32(1):33-37.
[22] 唐梅, 蔡松, 杨东成, 等. 不依赖IPTG诱导产木糖醇大肠杆菌工程菌的构建[J]. 中国酿造, 2021, 40(9):173-179.
TANG M, CAI S, YANG D C, et al. Construction of IPTG-inducing independent engineered Escherichia coli for xylitol production[J]. China Brewing, 2021, 40(9):173-179.
[23] 沈瑞华, 郭军玲, 周哲敏. 腈水合酶的稳定性改造研究进展[J]. 生物加工过程, 2020, 18(3):269-276.
SHEN R H, GUO J L, ZHOU Z M. Recent advances in stability reconstruction of nitrile hydratase[J]. Chinese Journal of Bioprocess Engineering, 2020, 18(3):269-276.
[24] 史悦, 于慧敏, 田卓玲, 等. 产腈水合酶重组大肠杆菌的质粒稳定性研究[J]. 中国生物工程杂志, 2005, 25(8):70-75.
SHI Y, YU H M, TIAN Z L, et al. Study on plasmid stability of a recombinant Escherichia coli producing nitrile hydratase[J]. China Biotechnology, 2005, 25(8):70-75.
[25] 王丽燕, 王煜, 吴坚平, 等. 腈水合酶NHaseK在大肠杆菌中的功能表达[J]. 中国生物工程杂志, 2016, 36(12):42-48.
WANG L Y, WANG Y, WU J P, et al. Functional expression of a nitrile hydratase from Klebsiella oxytoca KCTC 1686 in E.coli[J]. China Biotechnology, 2016, 36(12):42-48.
[26] 李彤, 呼海娟, 崔炜. 慢性心力衰竭患者脑钠肽抵抗机制的研究进展[J]. 心血管病学进展, 2023, 44(3):238-242.
LI T, HU H J, CUI W. Mechanism of brain natriuretic peptide tolerance in patients with chronic heart failure[J]. Advances in Cardiovascular Diseases, 2023, 44(3):238-242.
[27] 易俊波, 买制刚, 卢海蓉, 等. 多拷贝脑钠肽基因表达质粒的构建及其表达[J]. 中国生物制品学杂志, 2008, 21(12):1062-1065.
YI J B, MAI Z G, LU H R, et al. Construction and expression of recombinant expression vector for multi-copy B-type natriuretic peptide gene[J]. Chinese Journal of Biologicals, 2008, 21(12):1062-1065.
[28] TALERO E, GARCÍA-MAURIÑO S, ÁVILA-ROMÁN J, et al. Bioactive compounds isolated from microalgae in chronic inflammation and cancer[J]. Marine Drugs, 2015, 13(10):6152-6209.
[29] WANG F F, HUANG L D, GAO B Y, et al. Optimum production conditions, purification, identification, and antioxidant activity of violaxanthin from microalga Eustigmatos Cf. polyphem (Eustigmatophyceae)[J]. Marine Drugs, 2018, 16(6):190.
[30] DONG X R, LIU B, BAO Y H, et al. Metabolic engineering of Escherichia coli for high-level production of violaxanthin[J]. Microbial Cell Factories, 2023, 22(1):115.
[31] LI Y, LIN P X, LU X, et al. Plasmid copy number engineering accelerates fungal polyketide discovery upon unnatural polyketide biosynthesis[J]. ACS Synthetic Biology, 2023, 12(8):2226-2235.
[32] 郜彦彦, 魏明珠, 陈晨, 等. 紫色杆菌素的生物活性及其对草鱼的保鲜效果[J]. 食品研究与开发, 2023, 44(4):22-28.
GAO Y Y, WEI M Z, CHEN C, et al. Violacein: Bioactivity and preservation effect on grass carp[J]. Food Research and Development, 2023, 44(4):22-28.
[33] 卢丹妮, 洪沛雄, 张国超, 等. 基于低拷贝质粒的高产紫色杆菌素谷氨酸棒杆菌的构建和发酵[J]. 现代食品科技, 2023, 39(2):170-179.
LU D N, HONG P X, ZHANG G C, et al. Construction and fermentation of efficient violacein-producing Corynebacterium glutamicum strains using low copy number plasmids[J]. Modern Food Science and Technology, 2023, 39(2):170-179.
[34] SIMSA R, YUEN J, STOUT A, et al. Extracellular heme proteins influence bovine myosatellite cell proliferation and the color of cell-based meat[J]. Foods, 2019, 8(10):521.
[35] SUMAN S P, JOSEPH P. Myoglobin chemistry and meat color[J]. Annual Review of Food Science and Technology, 2013, 4:79-99.
[36] WANG M M, SHI Z, GAO N, et al. Sustainable and high-level microbial production of plant hemoglobin in Corynebacterium glutamicum[J]. Biotechnology for Biofuels and Bioproducts, 2023, 16(1):80.
[37] MEI C A, SCHWARTZ T J, SHANKS B H, et al. Triacetic acid lactone as a potential biorenewable platform chemical[J]. Green Chemistry, 2012, 14(7):1850-1853.
[38] YOCUM H C, BASSETT S, DA SILVA N A. Enhanced production of acetyl-CoA-based products via peroxisomal surface display in Saccharomyces cerevisiae[J]. Proceedings of the National Academy of Sciences of the United States of America, 2022, 119(48): e2214941119.
[39] 谢培琳, 张欣欣. 乙型肝炎表面抗原生物学功能的研究进展[J]. 中国病毒病杂志, 2020, 10(5): 333-336.
XIE P L, ZHANG X X. Research progress on hepatitis B virus surface antigen[J]. Chinese Journal of Viral Diseases, 2020, 10(5): 333-336.
[40] 何成, 蔡蓓蓓, 楼觉人. 多拷贝重组HBsAg质粒的构建及在毕赤酵母中的高效表达[J]. 中国生物制品学杂志, 2008, 21(3):207-211.
HE C, CAI B B, LOU J R. Construction of recombinant plasmid with multi-copy expression cassette for high expression of HBsAg in Pichia pastoris[J]. Chinese Journal of Biologicals, 2008, 21(3):207-211.
[41] LI C Y, ZOU Y S, JIANG T, et al. Harnessing plasmid replication mechanism to enable dynamic control of gene copy in bacteria[J]. Metabolic Engineering, 2022, 70:67-78.
[42] 曾薄轩. 在酿酒酵母中高产二氢青蒿酸[D]. 天津: 天津大学, 2021.
ZENG B X. High yield of dihydroartemisinin in Saccharomyces cerevisiae[D].Tianjin: Tianjin University, 2021.
[43] CHEN Y, PARTOW S, SCALCINATI G, et al. Enhancing the copy number of episomal plasmids in Saccharomyces cerevisiae for improved protein production[J]. FEMS Yeast Research, 2012, 12(5):598-607.
[44] WANG W F, XIAO H, ZHONG J J. Biosynthesis of a novel ganoderic acid by expressing CYP genes from Ganoderma lucidum in Saccharomyces cerevisiae[J]. Applied Microbiology and Biotechnology, 2022, 106(2):523-534.
[45] JOSHI S H N, YONG C T, GYORGY A. Inducible plasmid copy number control for synthetic biology in commonly used E. coli strains[J]. Nature Communications, 2022, 13(1):6691.
[46] 李星, 郝鹤, 池剑亭, 等. 利用异源生物生产青蒿素及其前体的研究进展[J]. 植物学报, 2012, 47(6):571-580.
LI X, HAO H, CHI J T, et al. An overview of heterologous organisms used to produce artemisinin and its precursors[J]. Chinese Bulletin of Botany, 2012, 47(6):571-580.
[47] YANG J, TIAN Y J, LIU H Y, et al. Harnessing the endogenous 2μ plasmid of Saccharomyces cerevisiae for pathway construction[J]. Frontiers in Microbiology, 2021, 12:679665.
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