食品与发酵工业 >

2024 , Vol. 50 >Issue 6: 323 - 331

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.035764

综述与专题评论

适配体传感器检测黄曲霉毒素的研究进展

  • 郭歌 ,
  • 王田林 ,
  • 李天歌 ,
  • 黄现青 ,
  • 陈云堂 ,
  • 王嘉楠 ,
  • 宋莲军
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  • 1(河南农业大学 食品科学技术学院,河南 郑州,450002)
    2(河南省科学院同位素研究所有限责任公司,河南 郑州,450002)
    3(中国农业大学 理学院,北京,100193)
第一作者:硕士研究生(宋莲军教授为通信作者,E-mail:slj69@126.com)

收稿日期: 2023-04-08

  修回日期: 2023-05-20

  网络出版日期: 2024-04-17

基金资助

河南省科技攻关项目(222102310331);国家自然科学基金项目(32101966);2022年河南省研究生联合培养基地项目(YJS2022JD16);河南省科学院重大科研聚焦项目(210104005);河南省科学院科技开放合作项目(220904004)

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Research progress of aptasensors for the detection of aflatoxins

  • GUO Ge ,
  • WANG Tianlin ,
  • LI Tiange ,
  • HUANG Xianqing ,
  • CHEN Yuntang ,
  • WANG Jianan ,
  • SONG Lianjun
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  • 1(College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China)
    2(Institute of Isotope Research, Henan Academy of Sciences, Zhengzhou 450002, China)
    3(College of Science, China Agricultural University, Beijing 100193, China)

Received date: 2023-04-08

  Revised date: 2023-05-20

  Online published: 2024-04-17

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摘要

黄曲霉毒素是自然界已发现理化性质最稳定的一类真菌毒素,具有高毒性和致癌性。农产品和动物源食品中黄曲霉毒素的污染最为严重。因此,开发快速精准检测黄曲霉毒素的方法对保障人和动物健康具有重要意义。适配体作为一种新型识别分子,其易于合成、修饰、稳定性高且高亲和力的特点使其在构建生物传感器用于食品分析检测中扮演着关键角色。适配体传感器是指适配体与待检目标物特异性识别的过程转换为易于检测的物理化学信号,以实现目标物的快速精准检测。该文综述了近年来基于电化学法、比色法、荧光法、拉曼光谱法等开发的适配体传感器在检测黄曲霉毒素中的研究进展,探讨了其在检测过程中的优势及局限性,并进一步对其在未来发展中的前景进行展望,为新型适配体传感器的开发用于黄曲霉毒素的检测提供了参考。

关键词： 黄曲霉毒素; 适配体; 传感器; 检测; 应用

本文引用格式

郭歌 , 王田林 , 李天歌 , 黄现青 , 陈云堂 , 王嘉楠 , 宋莲军 . 适配体传感器检测黄曲霉毒素的研究进展[J]. 食品与发酵工业, 2024 , 50(6) : 323 -331 . DOI: 10.13995/j.cnki.11-1802/ts.035764

Abstract

Aflatoxins are a class of fungal toxins known for their high toxicity and carcinogenic properties, and they are recognized as the most stable mycotoxins in terms of their physical and chemical characteristics.Contamination of aflatoxins in agricultural and animal-derived food products is a severe problem, highlighting the need for the development of rapid and accurate detection methods to safeguard human and animal health.Aptamers, as a novel class of recognition molecules, play a crucial role in the construction of biosensors for food analysis and detection.They possess advantages such as ease of synthesis, modification, high stability, and strong affinity, making them well-suited for these applications.Aptasensors refer to the conversion of the specific recognition process between aptamers and target analytes into detectable physicochemical signals, enabling rapid and precise detection of the target substances.This research content provides an overview of recent advancements in aptasensors for aflatoxin detection, focusing on electrochemical methods, colorimetric assays, fluorescence techniques, Raman spectroscopy, and other approaches.The advantages and limitations of aptasensors in the detection process are discussed, and future prospects for their development are explored.This research content serves as a valuable reference for the development of novel aptasensors for aflatoxin detection.

Key words： aflatoxin; aptamer; sensor; detection; application

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